FastQCFastQC Report
Fri 10 Feb 2017
ERR1632468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT200.8729812309035356TruSeq Adapter, Index 12 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA110.48013967699694454No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA110.48013967699694454No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC90.39284155390659103No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.3491924923614142No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA70.30554343081623747No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG70.30554343081623747No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT70.30554343081623747No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC70.30554343081623747RNA PCR Primer, Index 12 (95% over 23bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC70.30554343081623747No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG60.2618943692710607No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA60.2618943692710607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.2182453077258839No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.2182453077258839No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC50.2182453077258839No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA50.2182453077258839No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.2182453077258839No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT50.2182453077258839No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC50.2182453077258839No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT50.2182453077258839No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC50.2182453077258839No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT50.2182453077258839No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC50.2182453077258839No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC50.2182453077258839No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT40.1745962461807071No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC40.1745962461807071No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.1745962461807071No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC40.1745962461807071No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG40.1745962461807071No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC40.1745962461807071No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA40.1745962461807071No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC40.1745962461807071No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.1745962461807071No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA40.1745962461807071No Hit
GCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTT40.1745962461807071No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA40.1745962461807071No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC40.1745962461807071No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT40.1745962461807071No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT40.1745962461807071TruSeq Adapter, Index 12 (95% over 24bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40.1745962461807071No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC40.1745962461807071No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA30.13094718463553034No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCC30.13094718463553034No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGC30.13094718463553034No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG30.13094718463553034No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC30.13094718463553034No Hit
ACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGC30.13094718463553034No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC30.13094718463553034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT30.13094718463553034No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG30.13094718463553034No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC30.13094718463553034No Hit
CACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCT30.13094718463553034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30.13094718463553034No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCT30.13094718463553034No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG30.13094718463553034No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT30.13094718463553034No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT30.13094718463553034No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT30.13094718463553034No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT30.13094718463553034No Hit
CGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGATGAC30.13094718463553034No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA30.13094718463553034No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG30.13094718463553034No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA30.13094718463553034No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT30.13094718463553034No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA30.13094718463553034No Hit
GAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCG30.13094718463553034No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30.13094718463553034No Hit
TTTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT30.13094718463553034TruSeq Adapter, Index 12 (95% over 22bp)
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA30.13094718463553034No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.13094718463553034No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATG30.13094718463553034No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT30.13094718463553034No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT30.13094718463553034No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA30.13094718463553034No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG30.13094718463553034No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA30.13094718463553034No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers