##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632468.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2291
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48581405499782	34.0	31.0	34.0	31.0	34.0
2	32.65604539502401	34.0	31.0	34.0	31.0	34.0
3	32.70274989087735	34.0	31.0	34.0	31.0	34.0
4	36.16281099956351	37.0	35.0	37.0	35.0	37.0
5	36.073330423395895	37.0	35.0	37.0	35.0	37.0
6	36.058489742470535	37.0	36.0	37.0	35.0	37.0
7	36.109995635093846	37.0	36.0	37.0	35.0	37.0
8	36.0624181580096	37.0	35.0	37.0	35.0	37.0
9	37.823221300742034	39.0	38.0	39.0	35.0	39.0
10	37.815800960279354	39.0	38.0	39.0	35.0	39.0
11	37.92143168921868	39.0	38.0	39.0	35.0	39.0
12	37.790484504583155	39.0	38.0	39.0	35.0	39.0
13	37.93539938891314	39.0	38.0	39.0	35.0	39.0
14	39.27760803142733	40.0	39.0	41.0	36.0	41.0
15	39.218681798341336	40.0	39.0	41.0	36.0	41.0
16	39.10912265386294	40.0	39.0	41.0	36.0	41.0
17	39.23221300742034	40.0	39.0	41.0	36.0	41.0
18	39.21562636403317	40.0	39.0	41.0	36.0	41.0
19	39.23264949803579	40.0	39.0	41.0	36.0	41.0
20	39.20864251418595	40.0	39.0	41.0	36.0	41.0
21	39.122653862941945	40.0	39.0	41.0	36.0	41.0
22	39.077695329550416	40.0	39.0	41.0	36.0	41.0
23	38.97075512876473	40.0	39.0	41.0	35.0	41.0
24	39.073330423395895	40.0	39.0	41.0	36.0	41.0
25	39.01789611523352	40.0	39.0	41.0	35.0	41.0
26	39.016150152771715	40.0	39.0	41.0	35.0	41.0
27	38.76036665211698	40.0	38.0	41.0	35.0	41.0
28	38.82453077258839	40.0	38.0	41.0	35.0	41.0
29	38.6551724137931	40.0	38.0	41.0	35.0	41.0
30	38.64906154517678	40.0	38.0	41.0	35.0	41.0
31	38.61457878655609	40.0	38.0	41.0	35.0	41.0
32	38.54255783500655	40.0	38.0	41.0	35.0	41.0
33	38.61457878655609	40.0	38.0	41.0	35.0	41.0
34	38.42776080314273	40.0	38.0	41.0	34.0	41.0
35	38.352684417285026	40.0	38.0	41.0	34.0	41.0
36	38.19118288956788	40.0	38.0	41.0	34.0	41.0
37	38.16630292448713	40.0	37.0	41.0	33.0	41.0
38	38.050196420776956	40.0	37.0	41.0	33.0	41.0
39	37.79921431689219	40.0	37.0	41.0	33.0	41.0
40	37.71453513749454	40.0	36.0	41.0	33.0	41.0
41	37.65037101702313	40.0	36.0	41.0	33.0	41.0
42	37.5918812745526	40.0	36.0	41.0	33.0	41.0
43	36.885202968136184	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	1.0
25	7.0
26	1.0
27	6.0
28	11.0
29	15.0
30	20.0
31	39.0
32	41.0
33	40.0
34	51.0
35	109.0
36	156.0
37	268.0
38	587.0
39	937.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.68878219118289	17.19773024879965	13.793103448275861	26.320384111741596
2	23.090353557398515	19.38018332605849	30.510694020078567	27.018769096464425
3	21.562636403317327	19.51113051069402	29.63771278917503	29.28852029681362
4	16.28109995635094	16.41204714098647	33.56612832824094	33.74072457442165
5	17.89611523352248	32.60584897424705	30.11785246617198	19.38018332605849
6	32.82409428197294	33.52247926669577	16.58664338716718	17.06678306416412
7	29.11392405063291	27.19336534264513	18.507202095154955	25.185508511567
8	26.71322566564819	30.20515058926233	20.122217372326496	22.959406372762984
9	26.451331296377127	13.356612832824094	16.761239633347884	43.43081623745089
10	20.689655172413794	21.12614578786556	28.677433435181143	29.506765604539503
11	39.63334788302051	19.11828895678743	17.983413356612832	23.264949803579224
12	21.475338280226975	22.610213880401574	25.665648188563946	30.248799650807506
13	36.927106067219555	17.154081187254473	19.77302487996508	26.145787865560887
14	25.185508511567	20.122217372326496	22.391968572675687	32.30030554343082
15	31.427324312527283	23.17765168048887	18.594500218245308	26.800523788738538
16	25.44740288083806	24.87996508075076	20.602357049323437	29.070274989087736
17	26.71322566564819	24.006983849847227	19.729375818419904	29.550414666084677
18	26.887821911828897	19.903972064600612	22.915757311217806	30.29244871235268
19	29.768659973810564	22.21737232649498	21.518987341772153	26.494980357922305
20	29.94325621999127	19.423832387603667	22.65386294194675	27.979048450458315
21	29.899607158446095	20.73330423395897	20.820602357049324	28.546486250545613
22	27.586206896551722	22.610213880401574	18.943692710606722	30.85988651243998
23	29.463116542994328	21.78088171104321	22.173723264949803	26.582278481012654
24	28.9393278044522	20.20951549541685	22.566564818856392	28.28459188127455
25	27.19336534264513	22.7411610650371	23.264949803579224	26.800523788738538
26	30.074203404626797	23.04670449585334	22.130074203404625	24.749017896115234
27	26.320384111741596	23.264949803579224	24.312527280663467	26.102138804015713
28	26.669576604103014	23.003055434308163	22.697512003491923	27.6298559580969
29	25.752946311654302	23.657791357485813	22.173723264949803	28.415539065910085
30	25.31645569620253	23.3085988651244	24.26887821911829	27.10606721955478
31	28.371890004364907	21.562636403317327	20.689655172413794	29.375818419903972
32	24.487123526844172	21.344391095591444	23.919685726756875	30.248799650807506
33	25.53470100392842	21.868179834133567	23.264949803579224	29.332169358358794
34	27.018769096464425	21.38804015713662	22.959406372762984	28.63378437363597
35	24.443474465298994	24.181580096027936	25.927542557835004	25.44740288083806
36	27.237014404190308	22.435617634220865	23.264949803579224	27.062418158009606
37	27.498908773461373	22.042776080314272	23.74508948057617	26.71322566564819
38	26.800523788738538	20.34046268005238	25.31645569620253	27.542557835006548
39	27.80445220427761	18.68179834133566	27.45525971191619	26.058489742470535
40	25.752946311654302	18.9873417721519	29.419467481449146	25.84024443474465
41	22.872108249672632	20.384111741597554	27.76080314273243	28.98297686599738
42	20.646006110868615	21.12614578786556	31.514622435617635	26.71322566564819
43	18.81274552597119	21.69358358795286	30.46704495853339	29.02662592754256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	5.0
17	5.5
18	6.0
19	6.0
20	7.0
21	8.0
22	6.5
23	5.0
24	5.0
25	5.0
26	5.0
27	4.5
28	4.0
29	4.0
30	4.0
31	4.0
32	4.0
33	4.0
34	8.5
35	13.0
36	16.0
37	19.0
38	18.5
39	18.0
40	18.0
41	35.0
42	52.0
43	73.0
44	94.0
45	124.5
46	155.0
47	155.0
48	185.5
49	216.0
50	239.0
51	262.0
52	268.0
53	274.0
54	274.0
55	281.0
56	288.0
57	266.0
58	244.0
59	214.5
60	185.0
61	185.0
62	169.5
63	154.0
64	123.0
65	92.0
66	77.0
67	62.0
68	62.0
69	53.0
70	44.0
71	38.5
72	33.0
73	27.5
74	22.0
75	22.0
76	14.0
77	6.0
78	6.5
79	7.0
80	5.0
81	3.0
82	3.0
83	2.5
84	2.0
85	1.5
86	1.0
87	1.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2291.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.23876036665212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6696381751442	74.6398952422523
2	6.345044572627163	10.56307289393278
3	1.8353434714210803	4.583151462243562
4	0.8914525432616675	2.968136185072021
5	0.6292606187729418	2.6189436927106065
6	0.1048767697954903	0.5237887385421214
7	0.26219192448872575	1.5277171540811871
8	0.05243838489774515	0.3491924923614142
9	0.05243838489774515	0.39284155390659103
>10	0.15731515469323545	1.8332605848974246
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	20	0.8729812309035356	TruSeq Adapter, Index 12 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	11	0.48013967699694454	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	11	0.48013967699694454	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	9	0.39284155390659103	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.3491924923614142	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	7	0.30554343081623747	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	7	0.30554343081623747	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	7	0.30554343081623747	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	7	0.30554343081623747	RNA PCR Primer, Index 12 (95% over 23bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	7	0.30554343081623747	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	6	0.2618943692710607	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	6	0.2618943692710607	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.2182453077258839	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	5	0.2182453077258839	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	5	0.2182453077258839	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	5	0.2182453077258839	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	5	0.2182453077258839	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	5	0.2182453077258839	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	5	0.2182453077258839	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	5	0.2182453077258839	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	5	0.2182453077258839	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT	5	0.2182453077258839	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	5	0.2182453077258839	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	5	0.2182453077258839	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	4	0.1745962461807071	No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC	4	0.1745962461807071	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	4	0.1745962461807071	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	4	0.1745962461807071	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	4	0.1745962461807071	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	4	0.1745962461807071	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	4	0.1745962461807071	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	4	0.1745962461807071	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.1745962461807071	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	4	0.1745962461807071	No Hit
GCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTT	4	0.1745962461807071	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	4	0.1745962461807071	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	4	0.1745962461807071	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	4	0.1745962461807071	No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT	4	0.1745962461807071	TruSeq Adapter, Index 12 (95% over 24bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	4	0.1745962461807071	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	4	0.1745962461807071	No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA	3	0.13094718463553034	No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCC	3	0.13094718463553034	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGC	3	0.13094718463553034	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	3	0.13094718463553034	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	3	0.13094718463553034	No Hit
ACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGC	3	0.13094718463553034	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	3	0.13094718463553034	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	3	0.13094718463553034	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	3	0.13094718463553034	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	3	0.13094718463553034	No Hit
CACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCT	3	0.13094718463553034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.13094718463553034	No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCT	3	0.13094718463553034	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	3	0.13094718463553034	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	3	0.13094718463553034	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT	3	0.13094718463553034	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	3	0.13094718463553034	No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT	3	0.13094718463553034	No Hit
CGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGATGAC	3	0.13094718463553034	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	3	0.13094718463553034	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG	3	0.13094718463553034	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3	0.13094718463553034	No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT	3	0.13094718463553034	No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA	3	0.13094718463553034	No Hit
GAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCG	3	0.13094718463553034	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	3	0.13094718463553034	No Hit
TTTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	3	0.13094718463553034	TruSeq Adapter, Index 12 (95% over 22bp)
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA	3	0.13094718463553034	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	3	0.13094718463553034	No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATG	3	0.13094718463553034	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	3	0.13094718463553034	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	3	0.13094718463553034	No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA	3	0.13094718463553034	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	3	0.13094718463553034	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	3	0.13094718463553034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.08729812309035356	0.0
28	0.0	0.0	0.0	0.13094718463553034	0.0
29	0.0	0.0	0.0	0.30554343081623747	0.0
30	0.0	0.0	0.0	0.5237887385421214	0.0
31	0.0	0.0	0.0	1.003928415539066	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE