FastQCFastQC Report
Fri 10 Feb 2017
ERR1632467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81277
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1800.22146486705956173No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1530.18824513700062748No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1290.1587164880593526RNA PCR Primer, Index 17 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1180.14518252396126824No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1140.1402610824710558No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1080.13287892023573702No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1040.12795747874552457No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC920.1131931542748871No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA900.11073243352978086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCA303.5703744E-430.8333325
GTACTGG250.005466704329.5999981
GGTATCA1000.029.5999981
CTGGTTC450.003795744620.5555574
CTTGCAC450.003795744620.5555573
GGTTCAC450.003795744620.5555576
GGCTAAC851.2164364E-619.5882341
GTATCAA1551.8189894E-1219.0967752
CGCGGTA801.5867605E-518.522
GCGGTAA801.5867605E-518.523
GCACCCT500.00698013318.4999986
ACTCCGT1054.6631976E-717.6190476
GCTAACT953.5237117E-617.5263162
TTATACA752.0376392E-417.26666637
TCTTATA2200.016.81818237
AGCCGCG904.3647335E-516.44444519
CTAACTC904.3647335E-516.4444453
CGGTAAT904.3647335E-516.44444524
CTCCGTG956.9315196E-515.5789487
CGTGCCA956.9315196E-515.57894810