##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632467.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81277
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04494506440937	34.0	33.0	34.0	31.0	34.0
2	33.186719490138664	34.0	33.0	34.0	31.0	34.0
3	33.263604709819504	34.0	34.0	34.0	31.0	34.0
4	36.55433886585381	37.0	37.0	37.0	35.0	37.0
5	36.51614847988976	37.0	37.0	37.0	35.0	37.0
6	36.56357887225168	37.0	37.0	37.0	35.0	37.0
7	36.5651291263211	37.0	37.0	37.0	35.0	37.0
8	36.52952249713941	37.0	37.0	37.0	35.0	37.0
9	38.328604648301486	39.0	39.0	39.0	37.0	39.0
10	38.366081425249455	39.0	39.0	39.0	37.0	39.0
11	38.43522767818694	39.0	39.0	39.0	37.0	39.0
12	38.39932576251584	39.0	39.0	39.0	37.0	39.0
13	38.4278332123479	39.0	39.0	39.0	37.0	39.0
14	39.99475866481292	41.0	40.0	41.0	38.0	41.0
15	39.98543253318897	41.0	40.0	41.0	38.0	41.0
16	39.94752513011061	41.0	40.0	41.0	38.0	41.0
17	39.949432188688064	41.0	40.0	41.0	38.0	41.0
18	39.94862015084218	41.0	40.0	41.0	38.0	41.0
19	39.952274321148664	41.0	40.0	41.0	38.0	41.0
20	39.923582317260724	41.0	40.0	41.0	38.0	41.0
21	39.894016757508275	41.0	40.0	41.0	38.0	41.0
22	39.86803154643995	41.0	40.0	41.0	38.0	41.0
23	39.825522595568245	41.0	40.0	41.0	38.0	41.0
24	39.81544594411703	41.0	40.0	41.0	38.0	41.0
25	39.77313385090493	41.0	40.0	41.0	38.0	41.0
26	39.67723956346814	41.0	40.0	41.0	37.0	41.0
27	39.58955177971628	41.0	40.0	41.0	37.0	41.0
28	39.56665477318307	41.0	40.0	41.0	37.0	41.0
29	39.52031940155271	41.0	40.0	41.0	37.0	41.0
30	39.45806316670153	41.0	39.0	41.0	37.0	41.0
31	39.399227333686035	41.0	39.0	41.0	36.0	41.0
32	39.33478105737171	41.0	39.0	41.0	36.0	41.0
33	39.28003002079309	41.0	39.0	41.0	36.0	41.0
34	39.21755232107484	41.0	39.0	41.0	35.0	41.0
35	39.14075322662008	41.0	39.0	41.0	35.0	41.0
36	39.07169309890867	41.0	39.0	41.0	35.0	41.0
37	39.028753521906566	40.0	39.0	41.0	35.0	41.0
38	38.949210723821004	40.0	39.0	41.0	35.0	41.0
39	38.86927421041623	40.0	38.0	41.0	35.0	41.0
40	38.780356066291816	40.0	38.0	41.0	35.0	41.0
41	38.7162542908818	40.0	38.0	41.0	35.0	41.0
42	38.65025776049805	40.0	38.0	41.0	35.0	41.0
43	37.93803905163823	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	1.0
20	2.0
21	10.0
22	20.0
23	32.0
24	43.0
25	58.0
26	86.0
27	123.0
28	186.0
29	268.0
30	351.0
31	428.0
32	600.0
33	780.0
34	1164.0
35	1981.0
36	3195.0
37	6261.0
38	15996.0
39	49689.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36651205137985	16.10910835783801	12.243316067276105	28.281063523506035
2	21.96316301044576	18.93524613359253	31.243771300613947	27.857819555347763
3	21.45871525769898	19.289589920887828	28.127268476936894	31.124426344476298
4	16.275207008132682	15.270002583756781	32.25143644573495	36.20335396237558
5	17.680278553588348	32.50612104285345	32.62792671973621	17.185673683821992
6	36.41251522570961	32.38308500559814	14.505948792401293	16.698450976290953
7	30.95463661306397	27.384130811914808	18.80113685298424	22.860095722036984
8	28.183865054074335	30.574455257945054	19.378175867711654	21.863503820268956
9	27.50839720954267	12.82158544237607	16.678765210330106	42.99125213775115
10	19.989664972870553	23.432213295274185	28.38195307405539	28.19616865779987
11	40.18603048833003	18.91925144874934	18.03708306162875	22.85763500129188
12	23.65121744158864	22.942529866998044	25.84987142734107	27.55638126407225
13	35.746890264158374	16.64923656138883	20.33170515644032	27.272168018012476
14	24.893881417867295	18.523075408787236	21.94347724448491	34.63956592886056
15	29.862076602236797	23.707814018726083	18.852811988631473	27.577297390405647
16	27.193424954169075	23.005278245998255	21.147434083443038	28.653862716389632
17	27.653579733503943	23.114780319155482	20.775865250932	28.455774696408575
18	27.25002153130652	20.876754801481354	22.441773195368924	29.431450471843203
19	29.102944252371522	21.89672355032789	21.949629046347674	27.050703150952916
20	30.196734623571242	20.407987499538617	21.67402832289578	27.721249553994365
21	28.169100729603702	21.487013546267704	21.160968047541125	29.182917676587472
22	28.909777673880683	21.833975171327683	20.320631913087343	28.935615241704294
23	28.821191727056856	21.765074990464708	21.191727056854955	28.222006225623485
24	29.116478216469606	20.954267504952202	21.1511251645607	28.7781291140175
25	29.177996235097257	21.840126973190447	21.603897781660248	27.377979010052044
26	29.069724522312583	21.895493189955335	21.282773724423883	27.752008563308195
27	27.697872706915856	21.378741833483026	22.85886536166443	28.064520097936686
28	27.19834639565929	22.535280583682958	21.437799131365576	28.828573889292176
29	27.58837063375863	22.862556442782093	21.381202554228135	28.167870369231146
30	27.663422616484368	21.98284877640661	22.735829324409117	27.617899282699902
31	28.37334055144752	21.58544237607195	21.76138390934705	28.279833163133482
32	25.95322169863553	21.44641165397345	22.80349914489954	29.79686750249148
33	26.7098933277557	21.349213184541753	23.385459601117166	28.55543388658538
34	26.944892158913348	21.204030660580482	23.36577383515632	28.485303345349855
35	26.52779999261784	22.10834553440703	23.987105823295643	27.376748649679488
36	26.51426602851975	20.633143447715835	25.010765653259842	27.841824870504574
37	27.043320988717596	19.754666141712907	24.543228711689654	28.65878415787984
38	25.425397098810244	19.560269202849515	26.372774585676144	28.6415591126641
39	25.14364457349558	19.043517846377203	27.392743334522677	28.42009424560454
40	24.66749510931752	19.35479902063314	28.565276769565806	27.412429100483532
41	22.970828155566767	19.0226017200438	29.39946110215682	28.607109022232613
42	21.665415800287903	19.579954968810366	30.432963815101445	28.32166541580029
43	20.598693357284347	20.2246638040282	30.24594903847337	28.93069380021408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	3.0
7	3.0
8	4.0
9	5.0
10	5.5
11	6.0
12	6.0
13	9.5
14	13.0
15	29.5
16	46.0
17	43.5
18	41.0
19	41.0
20	49.0
21	57.0
22	63.0
23	69.0
24	88.0
25	107.0
26	107.0
27	128.5
28	150.0
29	243.0
30	336.0
31	425.0
32	514.0
33	514.0
34	648.0
35	782.0
36	894.0
37	1006.0
38	1372.5
39	1739.0
40	1739.0
41	2088.0
42	2437.0
43	2769.5
44	3102.0
45	4066.5
46	5031.0
47	5031.0
48	5728.0
49	6425.0
50	7227.0
51	8029.0
52	8562.5
53	9096.0
54	9096.0
55	8640.0
56	8184.0
57	7907.5
58	7631.0
59	7159.0
60	6687.0
61	6687.0
62	6279.5
63	5872.0
64	5027.5
65	4183.0
66	3694.0
67	3205.0
68	3205.0
69	2787.5
70	2370.0
71	2048.0
72	1726.0
73	1368.5
74	1011.0
75	1011.0
76	794.0
77	577.0
78	489.0
79	401.0
80	305.0
81	209.0
82	209.0
83	158.0
84	107.0
85	83.5
86	60.0
87	42.0
88	24.0
89	24.0
90	23.0
91	22.0
92	14.5
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	81277.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.2821585442376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60404584368959	63.871698020350166
2	5.166045291355979	7.364937190102981
3	1.7450289975144986	3.731683009953615
4	0.8992681579674123	2.5640710164007037
5	0.578224247445457	2.0608536240264774
6	0.3728251864125932	1.5945470428288446
7	0.2968793151063242	1.4813538885539574
8	0.20539906103286387	1.171303074670571
9	0.16915216790941728	1.0851778485918526
>10	0.8992681579674123	11.607219754666142
>50	0.05178127589063795	2.352449032321567
>100	0.01208229770781552	1.1147064975331273
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	180	0.22146486705956173	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	153	0.18824513700062748	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	129	0.1587164880593526	RNA PCR Primer, Index 17 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	118	0.14518252396126824	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	114	0.1402610824710558	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	108	0.13287892023573702	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	104	0.12795747874552457	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	92	0.1131931542748871	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	90	0.11073243352978086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0012303603725531207	0.0
20	0.0	0.0	0.0	0.0036910811176593624	0.0
21	0.0	0.0	0.0	0.0036910811176593624	0.0
22	0.0	0.0	0.0	0.004921441490212483	0.0
23	0.0	0.0	0.0	0.004921441490212483	0.0
24	0.0	0.0	0.0	0.006151801862765604	0.0
25	0.0	0.0	0.0	0.007382162235318725	0.0
26	0.0	0.0	0.0	0.012303603725531208	0.0
27	0.0	0.0	0.0	0.04675369415701859	0.0
28	0.0	0.0	0.0	0.18086297476530877	0.0
29	0.0	0.0	0.0	0.40478856256997675	0.0
30	0.0	0.0	0.0	0.6914625293748539	0.0
31	0.0	0.0	0.0	1.3780036172594954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTCA	30	3.5703744E-4	30.833332	5
GTACTGG	25	0.0054667043	29.599998	1
GGTATCA	100	0.0	29.599998	1
CTGGTTC	45	0.0037957446	20.555557	4
CTTGCAC	45	0.0037957446	20.555557	3
GGTTCAC	45	0.0037957446	20.555557	6
GGCTAAC	85	1.2164364E-6	19.588234	1
GTATCAA	155	1.8189894E-12	19.096775	2
CGCGGTA	80	1.5867605E-5	18.5	22
GCGGTAA	80	1.5867605E-5	18.5	23
GCACCCT	50	0.006980133	18.499998	6
ACTCCGT	105	4.6631976E-7	17.619047	6
GCTAACT	95	3.5237117E-6	17.526316	2
TTATACA	75	2.0376392E-4	17.266666	37
TCTTATA	220	0.0	16.818182	37
AGCCGCG	90	4.3647335E-5	16.444445	19
CTAACTC	90	4.3647335E-5	16.444445	3
CGGTAAT	90	4.3647335E-5	16.444445	24
CTCCGTG	95	6.9315196E-5	15.578948	7
CGTGCCA	95	6.9315196E-5	15.578948	10
>>END_MODULE