FastQCFastQC Report
Fri 10 Feb 2017
ERR1632466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72825
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4570.6275317542052867No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3210.4407826982492276No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2650.3638860281496739No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2580.35427394438722964No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2510.34466186062478543No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2250.30895983522142123No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2010.2760041194644696No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1970.2705115001716443No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1890.25952626158599384No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1830.2512873326467559No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1830.2512873326467559No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1810.24854102300034328No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1710.23480947476828012No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1690.2320631651218675No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1670.22931685547545486No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1560.21421215242018538No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1550.21283899759697908No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1550.21283899759697908No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1550.21283899759697908No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1520.2087195331273601No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1480.20322691383453484No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT1470.20185375901132854TruSeq Adapter, Index 11 (95% over 23bp)
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1450.19910744936491592No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1380.18949536560247168No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1360.18674905595605903No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1320.18125643666323377No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1300.17851012701682115No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1300.17851012701682115No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1280.17576381737040853No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1230.16889804325437693No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1220.1675248884311706No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1210.1661517336079643No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1140.1565396498455201No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1120.15379334019910745No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1090.1496738757294885No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1070.14692756608307586No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1070.14692756608307586No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1060.14555441125986956No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1060.14555441125986956No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1060.14555441125986956No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1030.14143494679025062No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1020.1400617919670443No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1020.1400617919670443No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1000.13731548232063165No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA970.1331960178510127No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG950.13044970820460006No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG940.12907655338139373No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC940.12907655338139373No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC940.12907655338139373No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC940.12907655338139373No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA920.1263302437349811No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG900.12358393408856848No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA890.12221077926536217No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA880.12083762444215584No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA880.12083762444215584No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG870.11946446961894953No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA870.11946446961894953No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG860.11809131479574321No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA850.1167181599725369No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC830.11397185032612427No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA820.11259869550291794No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC820.11259869550291794No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT820.11259869550291794No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC810.11122554067971163No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT800.10985238585650532No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC790.108479231033299No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC790.108479231033299No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA790.108479231033299No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG780.10710607621009269No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT760.10435976656368005No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC760.10435976656368005No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA750.10298661174047373No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC730.1002403020940611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGAGC601.2296368E-930.83333234
TGAGCAA551.8057108E-830.27272836
ATGAGCA551.8057108E-830.27272835
GCGGTAA1750.029.623
GGTATCA1250.029.5999981
GTCAATG900.028.77777731
CGCGGTA1800.028.77777722
CAATGAG652.8921932E-928.46153833
AACGTCA1000.027.74999828
CGTCAAT950.027.26315930
GGTAATA1900.027.26315925
TCAATGA754.4383341E-1027.13333332
GCAGCCG2050.027.07317217
CGTGCCA2050.027.07317210
AGCCGCG2050.027.07317219
TAATACG1950.026.56410427
CGGTAAT1950.026.56410424
CCGTGCC2100.026.428579
CAGCAGC2100.026.4285715
GTGCCAG2100.026.4285711
Produced by FastQC (version 0.11.5)