##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72825
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00366632337796	34.0	33.0	34.0	31.0	34.0
2	33.13165808444902	34.0	33.0	34.0	31.0	34.0
3	33.202540336422935	34.0	33.0	34.0	31.0	34.0
4	36.4911774802609	37.0	37.0	37.0	35.0	37.0
5	36.47513903192585	37.0	37.0	37.0	35.0	37.0
6	36.517102643323035	37.0	37.0	37.0	35.0	37.0
7	36.53434946790251	37.0	37.0	37.0	35.0	37.0
8	36.497809818056986	37.0	37.0	37.0	35.0	37.0
9	38.30810847923103	39.0	39.0	39.0	37.0	39.0
10	38.347861311362855	39.0	39.0	39.0	37.0	39.0
11	38.41952626158599	39.0	39.0	39.0	37.0	39.0
12	38.3564435290079	39.0	39.0	39.0	37.0	39.0
13	38.40556127703398	39.0	39.0	39.0	37.0	39.0
14	39.91873669756265	41.0	40.0	41.0	38.0	41.0
15	39.93849639546859	41.0	40.0	41.0	38.0	41.0
16	39.87450738070717	41.0	40.0	41.0	38.0	41.0
17	39.87859938208033	41.0	40.0	41.0	38.0	41.0
18	39.90495022313766	41.0	40.0	41.0	38.0	41.0
19	39.897590113285275	41.0	40.0	41.0	38.0	41.0
20	39.88653621695846	41.0	40.0	41.0	38.0	41.0
21	39.84785444558874	41.0	40.0	41.0	38.0	41.0
22	39.803776175763815	41.0	40.0	41.0	38.0	41.0
23	39.79184346035015	41.0	40.0	41.0	38.0	41.0
24	39.757459663577066	41.0	40.0	41.0	38.0	41.0
25	39.728032955715754	41.0	40.0	41.0	38.0	41.0
26	39.62912461380021	41.0	40.0	41.0	37.0	41.0
27	39.501036731891524	41.0	39.0	41.0	37.0	41.0
28	39.47887401304497	41.0	39.0	41.0	37.0	41.0
29	39.43447991761071	41.0	39.0	41.0	37.0	41.0
30	39.39553724682458	41.0	39.0	41.0	36.0	41.0
31	39.30782011671816	41.0	39.0	41.0	36.0	41.0
32	39.27437006522485	41.0	39.0	41.0	36.0	41.0
33	39.183604531410914	41.0	39.0	41.0	35.0	41.0
34	39.15302437349811	41.0	39.0	41.0	35.0	41.0
35	39.04212838997597	41.0	39.0	41.0	35.0	41.0
36	38.98271198077583	40.0	39.0	41.0	35.0	41.0
37	38.916882938551325	40.0	39.0	41.0	35.0	41.0
38	38.824730518365946	40.0	38.0	41.0	35.0	41.0
39	38.677816683831104	40.0	38.0	41.0	35.0	41.0
40	38.60620665980089	40.0	38.0	41.0	35.0	41.0
41	38.5397871610024	40.0	38.0	41.0	35.0	41.0
42	38.44624785444559	40.0	37.0	41.0	35.0	41.0
43	37.70326124270512	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	1.0
20	5.0
21	3.0
22	14.0
23	22.0
24	49.0
25	54.0
26	79.0
27	132.0
28	174.0
29	229.0
30	352.0
31	406.0
32	592.0
33	810.0
34	1204.0
35	1894.0
36	2901.0
37	5888.0
38	15502.0
39	42511.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.170614486783386	15.601785101270169	12.601441812564367	28.62615859938208
2	22.60212838997597	19.321661517336082	30.4346035015448	27.64160659114315
3	22.464812907655336	18.331616889804327	29.01064194987985	30.19292825266049
4	15.5647099210436	16.359766563680054	35.435633367662206	32.63989014761414
5	17.407483693786475	31.951939581187776	32.93923789907312	17.701338825952625
6	31.029179539993134	34.67627875042911	16.57397871610024	17.720562993477515
7	28.731891520768965	27.469962238242363	19.26536216958462	24.532784071404052
8	26.640576725025745	28.733264675592174	21.627188465499486	22.998970133882597
9	28.337796086508753	11.815997253690353	17.027119807758325	42.819086852042574
10	20.538276690696875	20.88705801579128	27.143151390319257	31.431513903192588
11	40.62341228973567	18.760041194644696	17.840027463096465	22.776519052523174
12	21.520082389289392	23.388946103673188	26.489529694473053	28.601441812564367
13	37.86749055956059	16.545142464812905	19.01956745623069	26.567799519395813
14	24.087881908685205	20.46961894953656	22.636457260556128	32.80604188122211
15	31.500171644352903	22.581531067627875	18.813594232749743	27.10470305526948
16	24.54514246481291	23.722622725712323	22.8479231033299	28.884311706144867
17	28.08925506350841	23.11980775832475	20.120837624442157	28.670099553724683
18	27.148644009612084	19.78441469275661	22.010298661174048	31.05664263645726
19	29.0435976656368	21.86749055956059	22.201167181599725	26.887744593202882
20	32.378990731204944	18.90696876072777	21.167181599725367	27.546858908341914
21	28.203226913834534	20.661860624785444	20.62615859938208	30.50875386199794
22	30.485410230003435	21.50635084105733	19.67593546172331	28.33230346721593
23	29.991074493649162	20.255406797116375	21.11500171644353	28.638516992790937
24	28.368005492619293	19.907998626845178	22.302780638516992	29.42121524201854
25	29.916924133196016	20.994164092001373	21.647785787847578	27.44112598695503
26	29.481634054239613	22.22588396841744	21.094404394095434	27.19807758324751
27	27.74733951253004	20.622039134912463	24.698935805012017	26.931685547545488
28	26.762787504291108	22.964641263302436	20.236182629591486	30.036388602814966
29	28.494335736354277	23.604531410916582	20.318571918983867	27.58256093374528
30	27.544112598695502	20.79505664263646	23.68692070030896	27.97391005835908
31	29.732921386886375	21.277033985581877	21.764503947820117	27.225540679711635
32	26.69138345348438	20.82114658427738	21.882595262615858	30.604874699622382
33	26.67215928595949	21.035358736697564	23.72536903535874	28.567112941984206
34	26.775145897699964	21.28664606934432	22.733951253003777	29.204256779951937
35	25.948506694129765	23.76930998970134	23.704771713010643	26.577411603158257
36	27.380707174733953	21.071060762100927	24.389975969790594	27.15825609337453
37	28.226570545829045	21.049090284929626	23.68692070030896	27.037418468932373
38	27.101956745623067	20.221077926536214	24.6961894953656	27.980775832475114
39	26.385169927909374	19.22828698935805	26.06934431857192	28.31719876416066
40	26.304153793340202	20.54788877445932	26.960521798832822	26.187435633367663
41	23.475454857535187	19.599038791623755	27.99038791623756	28.935118434603503
42	21.391005835907997	20.499828355647097	29.934775145897703	28.1743906625472
43	20.598695502917955	22.03638860281497	28.377617576381738	28.98729831788534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	3.0
9	4.0
10	6.0
11	8.0
12	8.0
13	21.5
14	35.0
15	54.5
16	74.0
17	62.0
18	50.0
19	50.0
20	64.5
21	79.0
22	54.0
23	29.0
24	28.0
25	27.0
26	27.0
27	26.0
28	25.0
29	22.0
30	19.0
31	25.5
32	32.0
33	32.0
34	87.0
35	142.0
36	140.5
37	139.0
38	303.0
39	467.0
40	467.0
41	774.5
42	1082.0
43	1540.5
44	1999.0
45	3694.0
46	5389.0
47	5389.0
48	6333.5
49	7278.0
50	8368.0
51	9458.0
52	9682.0
53	9906.0
54	9906.0
55	8983.5
56	8061.0
57	7835.0
58	7609.0
59	7032.0
60	6455.0
61	6455.0
62	5884.0
63	5313.0
64	4159.0
65	3005.0
66	2599.0
67	2193.0
68	2193.0
69	1923.0
70	1653.0
71	1334.5
72	1016.0
73	808.5
74	601.0
75	601.0
76	465.0
77	329.0
78	262.5
79	196.0
80	145.0
81	94.0
82	94.0
83	66.5
84	39.0
85	26.5
86	14.0
87	7.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	72825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.295571575695156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49159530605773	34.8492962581531
2	7.738661592134475	6.700995537246825
3	3.2064700285442433	4.164778578784758
4	1.9156359023152554	3.317542052866461
5	1.2020298128766256	2.6021283899759697
6	0.8594988899460831	2.232749742533471
7	0.5359974627339041	1.6244421558530724
8	0.5137963843958135	1.7796086508753861
9	0.3203298445924516	1.2481977342945416
>10	2.7909927053599746	23.41091658084449
>50	0.2886140183951792	8.505320974939924
>100	0.1363780526482715	9.564023343631995
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	457	0.6275317542052867	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	321	0.4407826982492276	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	265	0.3638860281496739	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	258	0.35427394438722964	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	251	0.34466186062478543	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	225	0.30895983522142123	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	201	0.2760041194644696	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	197	0.2705115001716443	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	189	0.25952626158599384	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	183	0.2512873326467559	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	183	0.2512873326467559	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	181	0.24854102300034328	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	171	0.23480947476828012	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	169	0.2320631651218675	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	167	0.22931685547545486	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	156	0.21421215242018538	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	155	0.21283899759697908	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	155	0.21283899759697908	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	155	0.21283899759697908	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	152	0.2087195331273601	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	148	0.20322691383453484	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	147	0.20185375901132854	TruSeq Adapter, Index 11 (95% over 23bp)
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	145	0.19910744936491592	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	138	0.18949536560247168	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	136	0.18674905595605903	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	132	0.18125643666323377	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	130	0.17851012701682115	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	130	0.17851012701682115	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	128	0.17576381737040853	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	123	0.16889804325437693	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	122	0.1675248884311706	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	121	0.1661517336079643	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	114	0.1565396498455201	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	112	0.15379334019910745	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	109	0.1496738757294885	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	107	0.14692756608307586	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	107	0.14692756608307586	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	106	0.14555441125986956	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	106	0.14555441125986956	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	106	0.14555441125986956	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	103	0.14143494679025062	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	102	0.1400617919670443	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	102	0.1400617919670443	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	100	0.13731548232063165	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	97	0.1331960178510127	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	95	0.13044970820460006	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	94	0.12907655338139373	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	94	0.12907655338139373	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	94	0.12907655338139373	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	94	0.12907655338139373	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	92	0.1263302437349811	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	90	0.12358393408856848	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	89	0.12221077926536217	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	88	0.12083762444215584	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	88	0.12083762444215584	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	87	0.11946446961894953	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	87	0.11946446961894953	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	86	0.11809131479574321	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	85	0.1167181599725369	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	83	0.11397185032612427	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	82	0.11259869550291794	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	82	0.11259869550291794	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	82	0.11259869550291794	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	81	0.11122554067971163	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	80	0.10985238585650532	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	79	0.108479231033299	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	79	0.108479231033299	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	79	0.108479231033299	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	78	0.10710607621009269	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	76	0.10435976656368005	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	76	0.10435976656368005	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	75	0.10298661174047373	No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC	73	0.1002403020940611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0013731548232063166	0.0	0.0	0.0	0.0
16	0.0013731548232063166	0.0	0.0	0.0027463096464126332	0.0
17	0.0013731548232063166	0.0	0.0	0.004119464469618949	0.0
18	0.0013731548232063166	0.0	0.0	0.0054926192928252664	0.0
19	0.0013731548232063166	0.0	0.0	0.0054926192928252664	0.0
20	0.0013731548232063166	0.0	0.0	0.006865774116031583	0.0
21	0.0013731548232063166	0.0	0.0	0.009612083762444216	0.0
22	0.0013731548232063166	0.0	0.0	0.010985238585650533	0.0
23	0.0013731548232063166	0.0	0.0	0.013731548232063165	0.0
24	0.0013731548232063166	0.0	0.0	0.017851012701682115	0.0
25	0.0013731548232063166	0.0	0.0	0.017851012701682115	0.0
26	0.0013731548232063166	0.0	0.0	0.02059732234809475	0.0
27	0.0013731548232063166	0.0	0.0	0.04394095434260213	0.0
28	0.0013731548232063166	0.0	0.0	0.13182286302780638	0.0
29	0.0013731548232063166	0.0	0.0	0.3117061448678338	0.0
30	0.0013731548232063166	0.0	0.0	0.6275317542052866	0.0
31	0.0013731548232063166	0.0	0.0	1.167181599725369	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGAGC	60	1.2296368E-9	30.833332	34
TGAGCAA	55	1.8057108E-8	30.272728	36
ATGAGCA	55	1.8057108E-8	30.272728	35
GCGGTAA	175	0.0	29.6	23
GGTATCA	125	0.0	29.599998	1
GTCAATG	90	0.0	28.777777	31
CGCGGTA	180	0.0	28.777777	22
CAATGAG	65	2.8921932E-9	28.461538	33
AACGTCA	100	0.0	27.749998	28
CGTCAAT	95	0.0	27.263159	30
GGTAATA	190	0.0	27.263159	25
TCAATGA	75	4.4383341E-10	27.133333	32
GCAGCCG	205	0.0	27.073172	17
CGTGCCA	205	0.0	27.073172	10
AGCCGCG	205	0.0	27.073172	19
TAATACG	195	0.0	26.564104	27
CGGTAAT	195	0.0	26.564104	24
CCGTGCC	210	0.0	26.42857	9
CAGCAGC	210	0.0	26.42857	15
GTGCCAG	210	0.0	26.42857	11
>>END_MODULE