FastQCFastQC Report
Fri 10 Feb 2017
ERR1632465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56907
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2830.4973026165498094No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2060.3619941307747729No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1810.31806280422443634No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1770.31103379197638253No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1610.28291774298416716TruSeq Adapter, Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1570.2758887307361133No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1510.2653452123640326No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1490.26183070624000565No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1450.2548016939919518No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1390.244258175619871No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1330.23371465724779023No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1240.2178993796896691No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1180.20735586131758835No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1170.20559860825557488No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1160.20384135519356142No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1120.19681234294550756No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1100.19329783682148066No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1090.1915405837594672No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1070.18802607763544027No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1060.1862688245734268No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1060.1862688245734268No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA980.17221080007731915No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA970.17045354701530568No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA960.16869629395329222No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC930.16342453476725183No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC910.1599100286432249No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC910.1599100286432249No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA900.15815277558121146No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC890.156395522519198No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC880.15463826945718454No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA860.1511237633331576No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC850.14936651027114414No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC850.14936651027114414No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA840.14760925720913068No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC810.1423374980230903No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC810.1423374980230903No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG790.13882299189906339No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG790.13882299189906339No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC780.13706573883704992No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG770.13530848577503646No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT770.13530848577503646No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA760.13355123271302302No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA750.13179397965100956No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT750.13179397965100956No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC740.1300367265889961No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG730.12827947352698263No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT720.12652222046496917No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT720.12652222046496917No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC710.1247649674029557No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA690.12125046127892877No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG690.12125046127892877No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG680.11949320821691531No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA680.11949320821691531No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT670.11773595515490184No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA660.11597870209288841No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA660.11597870209288841No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC660.11597870209288841No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA660.11597870209288841No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC650.11422144903087494No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC640.11246419596886148No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA610.1071924367828211No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG610.1071924367828211No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG590.10367793065879417No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA590.10367793065879417No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC580.10192067759678071No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT580.10192067759678071No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT580.10192067759678071No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC580.10192067759678071No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG580.10192067759678071No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG570.10016342453476725No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG570.10016342453476725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT251.2189395E-437.036
GTATGCC303.557029E-430.83333432
TGCCGTC303.557029E-430.83333435
TATGCCG303.557029E-430.83333433
ATGCCGT303.557029E-430.83333434
CGTATGC303.557029E-430.83333431
TCGTATG303.557029E-430.83333430
CTCGTAT303.557029E-430.83333429
CGTGCCA801.8189894E-1230.062510
GGTATCA1050.029.952381
TAACCTT250.005453090629.65
GCAGCCG851.8189894E-1228.29411717
GTGCCAG851.8189894E-1228.29411711
CCGTGCC905.456968E-1226.7222219
CCAGCAG905.456968E-1226.72222114
CCGTCTT358.7657815E-426.42857237
TCCGTGC959.094947E-1225.315798
CCGCGGT959.094947E-1225.3157921
CAGCCGC959.094947E-1225.3157918
GCGGTAA901.3460522E-1024.66666623
Produced by FastQC (version 0.11.5)