##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632465.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56907
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6102236983148	34.0	31.0	34.0	31.0	34.0
2	32.770994780958404	34.0	31.0	34.0	31.0	34.0
3	32.845730753685835	34.0	31.0	34.0	31.0	34.0
4	36.30338974115662	37.0	37.0	37.0	35.0	37.0
5	36.185917373961026	37.0	35.0	37.0	35.0	37.0
6	36.198973764211786	37.0	36.0	37.0	35.0	37.0
7	36.226123324019895	37.0	36.0	37.0	35.0	37.0
8	36.169328905055615	37.0	36.0	37.0	35.0	37.0
9	37.87518231500518	39.0	38.0	39.0	35.0	39.0
10	37.874180680759835	39.0	38.0	39.0	35.0	39.0
11	37.95714059781749	39.0	38.0	39.0	35.0	39.0
12	37.8999771557102	39.0	38.0	39.0	35.0	39.0
13	37.960426661043456	39.0	38.0	39.0	35.0	39.0
14	39.284956156536104	40.0	39.0	41.0	36.0	41.0
15	39.298188272093064	40.0	39.0	41.0	37.0	41.0
16	39.213277804136574	40.0	39.0	41.0	36.0	41.0
17	39.23230885479818	40.0	39.0	41.0	36.0	41.0
18	39.25462596868575	40.0	39.0	41.0	36.0	41.0
19	39.244855641660955	40.0	39.0	41.0	36.0	41.0
20	39.23657897973887	40.0	39.0	41.0	36.0	41.0
21	39.20417523327534	40.0	39.0	41.0	36.0	41.0
22	39.127769870139	40.0	39.0	41.0	36.0	41.0
23	39.09620960514523	40.0	39.0	41.0	36.0	41.0
24	39.066301158029766	40.0	39.0	41.0	36.0	41.0
25	39.02614792556276	40.0	38.0	41.0	36.0	41.0
26	38.89948512485283	40.0	38.0	41.0	35.0	41.0
27	38.75447660217548	40.0	38.0	41.0	35.0	41.0
28	38.75171771486812	40.0	38.0	41.0	35.0	41.0
29	38.666701811727904	40.0	38.0	41.0	35.0	41.0
30	38.60730665823185	40.0	38.0	41.0	35.0	41.0
31	38.536629940077674	40.0	38.0	41.0	35.0	41.0
32	38.48818247315796	40.0	38.0	41.0	35.0	41.0
33	38.38301087739645	40.0	38.0	41.0	35.0	41.0
34	38.36282003971392	40.0	38.0	41.0	34.0	41.0
35	38.23499745198306	40.0	38.0	41.0	34.0	41.0
36	38.175918604038166	40.0	38.0	41.0	34.0	41.0
37	38.134271706468446	40.0	38.0	41.0	34.0	41.0
38	38.034336724831746	40.0	37.0	41.0	34.0	41.0
39	37.910221941061735	40.0	37.0	41.0	33.0	41.0
40	37.8218672571037	40.0	37.0	41.0	33.0	41.0
41	37.73627146045302	40.0	37.0	41.0	33.0	41.0
42	37.672500746832554	40.0	36.0	41.0	33.0	41.0
43	36.86701108826682	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	6.0
21	10.0
22	20.0
23	31.0
24	56.0
25	68.0
26	107.0
27	173.0
28	193.0
29	283.0
30	393.0
31	575.0
32	678.0
33	994.0
34	1422.0
35	2295.0
36	3967.0
37	8097.0
38	18325.0
39	19212.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.082696329098354	16.237018293004375	13.571265397929954	28.109019979967314
2	22.59300261830706	19.70407858435693	30.86790728732845	26.835011510007554
3	22.21167870385014	18.786792485985906	28.982374751788004	30.019154058375946
4	15.737958423392554	17.03129667703446	34.763737325812286	32.4670075737607
5	17.85544836311877	31.850211748993974	32.41956174108633	17.87477814680092
6	31.535663450893566	34.60382729716906	16.005060888818598	17.85544836311877
7	28.576449294462897	27.52561196337885	19.44400513117894	24.453933610979316
8	26.488832656790905	29.437503294849492	21.180171156448242	22.893492891911364
9	28.117806245277382	12.2585973606059	17.380990036375138	42.24260635774158
10	20.345475951991844	21.52459275660288	27.437749310278175	30.692181981127103
11	40.12863092413939	19.013478130985643	17.85017660393273	23.00771434094224
12	21.88482963431564	23.564763561600508	26.420299787372382	28.130107016711474
13	37.58940024952994	17.01548139947634	19.196232449435044	26.198885901558683
14	24.139385312878908	20.964029029820587	22.82144551636881	32.075140140931694
15	31.497003883529267	23.269545047182245	18.63566872265275	26.59778234663574
16	24.863373574428454	23.761575904546014	22.80035847962465	28.574692041400883
17	27.476408877642474	23.598151369778762	20.28572934788339	28.63971040469538
18	27.14253079585991	19.862231359938146	22.223979475284235	30.77125836891771
19	28.92614265380357	22.223979475284235	22.278454320206652	26.571423550705536
20	32.28601050837331	18.89574217583074	21.737220377106507	27.08102693868944
21	28.044001616672816	20.928883968580315	21.26100479730086	29.766109617446006
22	29.21960391515982	22.368074226369338	20.211924719278823	28.200397139192013
23	29.71514927864762	21.007960356370923	21.318994148347304	27.95789621663416
24	28.4200537719437	20.06782996819372	22.489324687648267	29.022791572214313
25	29.375999437679017	21.326023160595355	22.16071836505175	27.13725903667387
26	29.282865025392308	22.44539336109793	21.584339360711336	26.687402252798424
27	27.44653557558824	21.194229180944347	24.96880875814926	26.39042648531815
28	26.652257191558153	23.197497671639695	20.610821164355876	29.539423972446272
29	27.854218285975364	23.610452141212857	21.027290140053072	27.508039432758714
30	27.367459187797632	21.097580262533608	23.935543957685347	27.59941659198341
31	29.527123201012177	21.253975785052805	22.072855711951078	27.14604530198394
32	26.27796228934929	21.125696311525825	22.46999490396612	30.126346495158767
33	26.26214701179117	21.116910046215757	24.153443337375016	28.467499604618062
34	26.38691197919412	21.46484615249442	23.469871896251778	28.678369972059674
35	25.413393782838668	23.547191030980372	24.873917092800536	26.165498093380428
36	26.83852601613158	21.09933751559562	24.868645333614495	27.193491134658306
37	27.40963326128596	20.923612209394275	23.9689317658636	27.697822763456166
38	25.852707048342033	19.77436870683747	26.174284358690496	28.19863988613
39	25.497741929815316	18.667299277768993	27.41666227353401	28.418296518881686
40	25.18670813783893	20.189080429472646	28.223241428998193	26.400970003690233
41	22.54731403869471	19.015235384047656	29.607956841864798	28.829493735392838
42	20.826963290983535	19.98172456815506	30.917110373064826	28.27420176779658
43	19.70407858435693	21.361168221835626	29.64485915616708	29.289894037640362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	6.0
6	4.0
7	2.0
8	3.0
9	4.0
10	5.5
11	7.0
12	7.0
13	12.5
14	18.0
15	41.0
16	64.0
17	67.5
18	71.0
19	71.0
20	79.5
21	88.0
22	63.0
23	38.0
24	31.0
25	24.0
26	24.0
27	21.5
28	19.0
29	17.5
30	16.0
31	23.5
32	31.0
33	31.0
34	87.0
35	143.0
36	122.0
37	101.0
38	266.0
39	431.0
40	431.0
41	668.0
42	905.0
43	1269.5
44	1634.0
45	2984.0
46	4334.0
47	4334.0
48	5263.0
49	6192.0
50	7010.5
51	7829.0
52	7837.5
53	7846.0
54	7846.0
55	7162.5
56	6479.0
57	6228.0
58	5977.0
59	5407.0
60	4837.0
61	4837.0
62	4293.5
63	3750.0
64	2939.5
65	2129.0
66	1814.5
67	1500.0
68	1500.0
69	1277.0
70	1054.0
71	865.0
72	676.0
73	505.0
74	334.0
75	334.0
76	259.0
77	184.0
78	146.5
79	109.0
80	78.5
81	48.0
82	48.0
83	33.0
84	18.0
85	13.0
86	8.0
87	4.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	56907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.66749257560581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.813760197014	36.448943012283195
2	8.19224257349546	7.482383538053315
3	3.31691549946129	4.5442564183668095
4	1.9816838540865016	3.619941307747729
5	1.189010312451901	2.714955980810797
6	0.9196552254886871	2.5199008909273024
7	0.6233646298291519	1.992724972323264
8	0.5194705248576267	1.8978333069745372
9	0.450207788209943	1.850387474300174
>10	2.6589195013082962	23.654383467763193
>50	0.253963367708173	7.990229672975205
>100	0.08080652608896413	5.284059957474476
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	283	0.4973026165498094	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	206	0.3619941307747729	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	181	0.31806280422443634	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	177	0.31103379197638253	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	161	0.28291774298416716	TruSeq Adapter, Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	157	0.2758887307361133	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	151	0.2653452123640326	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	149	0.26183070624000565	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	145	0.2548016939919518	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	139	0.244258175619871	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	133	0.23371465724779023	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	124	0.2178993796896691	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	118	0.20735586131758835	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	117	0.20559860825557488	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	116	0.20384135519356142	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	112	0.19681234294550756	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	110	0.19329783682148066	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	109	0.1915405837594672	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	107	0.18802607763544027	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	106	0.1862688245734268	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	106	0.1862688245734268	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	98	0.17221080007731915	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	97	0.17045354701530568	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	96	0.16869629395329222	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	93	0.16342453476725183	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	91	0.1599100286432249	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	91	0.1599100286432249	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	90	0.15815277558121146	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	89	0.156395522519198	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	88	0.15463826945718454	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	86	0.1511237633331576	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	85	0.14936651027114414	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	85	0.14936651027114414	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	84	0.14760925720913068	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	81	0.1423374980230903	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	81	0.1423374980230903	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	79	0.13882299189906339	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	79	0.13882299189906339	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	78	0.13706573883704992	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	77	0.13530848577503646	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	77	0.13530848577503646	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	76	0.13355123271302302	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	75	0.13179397965100956	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	75	0.13179397965100956	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	74	0.1300367265889961	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	73	0.12827947352698263	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	72	0.12652222046496917	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	72	0.12652222046496917	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	71	0.1247649674029557	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	69	0.12125046127892877	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	69	0.12125046127892877	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	68	0.11949320821691531	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	68	0.11949320821691531	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	67	0.11773595515490184	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	66	0.11597870209288841	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	66	0.11597870209288841	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	66	0.11597870209288841	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	66	0.11597870209288841	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	65	0.11422144903087494	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	64	0.11246419596886148	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	61	0.1071924367828211	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	61	0.1071924367828211	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	59	0.10367793065879417	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	59	0.10367793065879417	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	58	0.10192067759678071	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	58	0.10192067759678071	No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT	58	0.10192067759678071	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	58	0.10192067759678071	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	58	0.10192067759678071	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	57	0.10016342453476725	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	57	0.10016342453476725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0017572530620134606	0.0
20	0.0017572530620134606	0.0	0.0	0.0035145061240269213	0.0
21	0.0035145061240269213	0.0	0.0	0.005271759186040382	0.0
22	0.0035145061240269213	0.0	0.0	0.008786265310067303	0.0
23	0.0035145061240269213	0.0	0.0	0.008786265310067303	0.0
24	0.0035145061240269213	0.0	0.0	0.014058024496107685	0.0
25	0.0035145061240269213	0.0	0.0	0.014058024496107685	0.0
26	0.0035145061240269213	0.0	0.0	0.021087036744161528	0.0
27	0.0035145061240269213	0.0	0.0	0.06150385717047112	0.0
28	0.0035145061240269213	0.0	0.0	0.20559860825557488	0.0
29	0.0035145061240269213	0.0	0.0	0.4182262287592036	0.0
30	0.0035145061240269213	0.0	0.0	0.7275027676735727	0.0
31	0.0035145061240269213	0.0	0.0	1.5182666455796299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	25	1.2189395E-4	37.0	36
GTATGCC	30	3.557029E-4	30.833334	32
TGCCGTC	30	3.557029E-4	30.833334	35
TATGCCG	30	3.557029E-4	30.833334	33
ATGCCGT	30	3.557029E-4	30.833334	34
CGTATGC	30	3.557029E-4	30.833334	31
TCGTATG	30	3.557029E-4	30.833334	30
CTCGTAT	30	3.557029E-4	30.833334	29
CGTGCCA	80	1.8189894E-12	30.0625	10
GGTATCA	105	0.0	29.95238	1
TAACCTT	25	0.0054530906	29.6	5
GCAGCCG	85	1.8189894E-12	28.294117	17
GTGCCAG	85	1.8189894E-12	28.294117	11
CCGTGCC	90	5.456968E-12	26.722221	9
CCAGCAG	90	5.456968E-12	26.722221	14
CCGTCTT	35	8.7657815E-4	26.428572	37
TCCGTGC	95	9.094947E-12	25.31579	8
CCGCGGT	95	9.094947E-12	25.31579	21
CAGCCGC	95	9.094947E-12	25.31579	18
GCGGTAA	90	1.3460522E-10	24.666666	23
>>END_MODULE