##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632464.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3159
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14023425134536	33.0	31.0	34.0	30.0	34.0
2	32.31433998100665	34.0	31.0	34.0	30.0	34.0
3	32.3738524849636	34.0	31.0	34.0	30.0	34.0
4	35.77049699271922	37.0	35.0	37.0	33.0	37.0
5	35.750870528648306	37.0	35.0	37.0	35.0	37.0
6	35.76923076923077	37.0	35.0	37.0	33.0	37.0
7	35.776511554289335	37.0	35.0	37.0	33.0	37.0
8	35.78695789806901	37.0	35.0	37.0	33.0	37.0
9	37.49351060462172	39.0	37.0	39.0	34.0	39.0
10	37.46280468502691	39.0	37.0	39.0	34.0	39.0
11	37.566635011079455	39.0	37.0	39.0	35.0	39.0
12	37.41310541310541	39.0	37.0	39.0	34.0	39.0
13	37.51915163026274	39.0	37.0	39.0	35.0	39.0
14	38.7730294396961	40.0	38.0	41.0	34.0	41.0
15	38.83602405824628	40.0	38.0	41.0	34.0	41.0
16	38.74010762899652	40.0	38.0	41.0	34.0	41.0
17	38.830959164292494	40.0	38.0	41.0	35.0	41.0
18	38.81798037353593	40.0	38.0	41.0	35.0	41.0
19	38.72649572649573	40.0	38.0	41.0	34.0	41.0
20	38.71351693573916	40.0	38.0	41.0	34.0	41.0
21	38.60620449509339	40.0	38.0	41.0	34.0	41.0
22	38.72238050015828	40.0	38.0	41.0	34.0	41.0
23	38.723963279518834	40.0	38.0	41.0	34.0	41.0
24	38.60367204811649	40.0	38.0	41.0	34.0	41.0
25	38.554922443811336	40.0	38.0	41.0	34.0	41.0
26	38.4764165875277	40.0	38.0	41.0	34.0	41.0
27	38.47704969927192	40.0	38.0	41.0	34.0	41.0
28	38.37860082304527	40.0	38.0	41.0	34.0	41.0
29	38.221589110478	40.0	38.0	41.0	33.0	41.0
30	38.13105413105413	40.0	37.0	41.0	33.0	41.0
31	38.08958531180753	40.0	37.0	41.0	33.0	41.0
32	38.03735359290915	40.0	37.0	41.0	33.0	41.0
33	37.89110477999367	40.0	37.0	41.0	33.0	41.0
34	37.844254510921175	40.0	37.0	41.0	32.0	41.0
35	37.71415004748338	40.0	37.0	41.0	32.0	41.0
36	37.730610952833175	40.0	37.0	41.0	32.0	41.0
37	37.6343779677113	40.0	37.0	41.0	31.0	41.0
38	37.59449192782526	40.0	37.0	41.0	32.0	41.0
39	37.451725229503005	40.0	36.0	41.0	31.0	41.0
40	37.19816397594175	40.0	36.0	41.0	31.0	41.0
41	37.09908198797088	40.0	36.0	41.0	31.0	41.0
42	37.02722380500158	40.0	36.0	41.0	31.0	41.0
43	36.22000633111744	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	0.0
23	3.0
24	7.0
25	5.0
26	9.0
27	18.0
28	26.0
29	44.0
30	54.0
31	74.0
32	98.0
33	107.0
34	140.0
35	116.0
36	188.0
37	303.0
38	624.0
39	1340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83000949667616	16.745805634694523	13.105413105413104	27.31877176321621
2	21.62076606521051	19.21494143716366	31.877176321620766	27.287116176005068
3	21.240899018676796	19.594808483697374	31.117442228553337	28.046850269072493
4	15.57454890788224	15.669515669515668	32.92181069958848	35.83412472301361
5	17.25229503007281	33.30167774612219	31.46565368787591	17.980373535929093
6	35.04273504273504	32.193732193732195	15.92276036720481	16.840772396327953
7	27.666983222538775	28.078505856283638	21.17758784425451	23.076923076923077
8	27.160493827160494	32.257043368154484	19.37321937321937	21.209243431465655
9	26.179170623615068	13.485280151946819	18.138651471984804	42.19689775245331
10	19.0250079138968	24.311490978157646	30.4210193099082	26.242481798037353
11	38.14498258942704	19.183285849952515	19.753086419753085	22.918645140867362
12	22.79202279202279	24.659702437480217	26.559037670148783	25.989237100348213
13	33.998100664767335	17.758784425451093	21.937321937321936	26.305792972459642
14	23.108578664134217	20.92434314656537	23.330167774612217	32.63691041468819
15	28.96486229819563	23.615068059512502	20.60778727445394	26.812282367837923
16	23.710034821145932	25.102880658436217	24.37480215257993	26.812282367837923
17	25.767647989870213	23.393478949034506	23.646723646723647	27.192149414371634
18	26.274137385248498	23.20354542576765	23.646723646723647	26.875593542260205
19	28.078505856283638	22.253877809433366	23.393478949034506	26.274137385248498
20	29.123140234251345	21.430832541943655	24.02659069325736	25.41943653054764
21	26.052548274770498	22.570433681544795	23.710034821145932	27.666983222538775
22	25.577714466603357	24.628046850269072	22.79202279202279	27.00221589110478
23	28.205128205128204	21.62076606521051	22.285533396644507	27.888572333016775
24	26.938904716682494	21.77904400126622	23.899968344412788	27.38208293763849
25	27.12883823994935	22.950300728078506	23.330167774612217	26.590693257359927
26	27.825261158594493	22.475466919911362	23.710034821145932	25.989237100348213
27	26.369104146881924	23.741690408357073	23.140234251345362	26.74897119341564
28	25.862614751503642	23.931623931623932	23.23520101297879	26.970560303893638
29	27.160493827160494	23.330167774612217	23.425134536245647	26.084203861981642
30	26.242481798037353	22.823678379233936	25.704336815447924	25.229503007280783
31	26.875593542260205	21.74738841405508	24.691358024691358	26.685660018993353
32	24.691358024691358	22.348844571066792	24.976258309591643	27.983539094650205
33	25.73599240265907	23.36182336182336	25.862614751503642	25.039569484013928
34	26.43241532130421	22.348844571066792	25.229503007280783	25.989237100348213
35	25.64102564102564	24.691358024691358	23.868312757201647	25.799303577081357
36	25.925925925925924	23.646723646723647	24.881291547958213	25.546058879392213
37	26.90724912947135	22.380500158277936	24.501424501424502	26.21082621082621
38	24.976258309591643	21.209243431465655	27.065527065527068	26.74897119341564
39	23.67837923393479	19.56315289648623	27.572016460905353	29.18645140867363
40	24.976258309591643	22.41215574548908	28.61665083887306	23.994935106046217
41	22.88698955365622	21.209243431465655	28.996517885406774	26.90724912947135
42	22.06394428616651	21.27255460588794	30.07280785058563	26.590693257359927
43	21.209243431465655	20.92434314656537	30.167774612219056	27.69863880974992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	2.0
9	1.0
10	1.5
11	2.0
12	2.0
13	3.5
14	5.0
15	10.5
16	16.0
17	15.0
18	14.0
19	14.0
20	15.5
21	17.0
22	15.0
23	13.0
24	16.5
25	20.0
26	20.0
27	17.0
28	14.0
29	17.0
30	20.0
31	28.0
32	36.0
33	36.0
34	42.5
35	49.0
36	52.5
37	56.0
38	66.5
39	77.0
40	77.0
41	91.0
42	105.0
43	126.0
44	147.0
45	194.0
46	241.0
47	241.0
48	259.0
49	277.0
50	299.5
51	322.0
52	321.5
53	321.0
54	321.0
55	310.5
56	300.0
57	297.0
58	294.0
59	260.5
60	227.0
61	227.0
62	205.0
63	183.0
64	149.0
65	115.0
66	101.0
67	87.0
68	87.0
69	84.0
70	81.0
71	63.5
72	46.0
73	38.0
74	30.0
75	30.0
76	24.0
77	18.0
78	14.0
79	10.0
80	8.5
81	7.0
82	7.0
83	4.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3159.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.73789173789174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.44444444444444	86.64134219689775
2	3.8302277432712217	7.027540360873694
3	0.8971704623878536	2.4691358024691357
4	0.3105590062111801	1.1396011396011396
5	0.27605244996549344	1.2662234884457106
6	0.1725327812284334	0.949667616334283
7	0.03450655624568668	0.22158911047799934
8	0.0	0.0
9	0.03450655624568668	0.2849002849002849
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.2849002849002849	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	7	0.22158911047799934	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	6	0.1899335232668566	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	6	0.1899335232668566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.1899335232668566	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	6	0.1899335232668566	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	6	0.1899335232668566	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	5	0.15827793605571383	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.15827793605571383	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	5	0.15827793605571383	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	5	0.15827793605571383	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	5	0.15827793605571383	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	5	0.15827793605571383	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	5	0.15827793605571383	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	5	0.15827793605571383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.12662234884457108	No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC	4	0.12662234884457108	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	4	0.12662234884457108	No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGT	4	0.12662234884457108	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	4	0.12662234884457108	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	4	0.12662234884457108	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	4	0.12662234884457108	No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATT	4	0.12662234884457108	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	4	0.12662234884457108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0949667616334283	0.0
29	0.0	0.0	0.0	0.25324469768914215	0.0
30	0.0	0.0	0.0	0.3482114593225704	0.0
31	0.0	0.0	0.0	0.6964229186451408	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE