FastQCFastQC Report
Fri 10 Feb 2017
ERR1632463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758398
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18370.24222110290375237No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13000.17141395415072297No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12870.16969981460921574No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11220.14794342812085476No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10780.14214172505729183No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9430.12434104520317828No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9300.12262690566167106No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9290.1224950487738628No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9130.12038533856893084No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8650.11405620795413489No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8510.11221021152481943No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8190.10799079111495546No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8190.10799079111495546No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8030.1058810809100235No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7980.10522179647098225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.028.8599991
TCTTGTA400.001930584723.1252
TATACAC3100.022.67741837
GATATGT450.003824626320.5555551
GAAATGA555.140875E-420.18181816
ATACGGC851.2441651E-619.58823629
CTTATAC10450.019.11961737
GTATCAA15050.018.6843872
GCGGTAA7050.018.63120723
ACTATAT500.007032728818.53
GCCATAG609.232867E-418.51
TCTTATA20550.018.00486637
CTCTATG2250.017.2666661
CGGTAAT7650.017.16993524
GTACTAT650.00157926417.0769231
AGTCGGT1757.2759576E-1216.91428611
CGCGGTA8000.016.8812522
ATACACA1554.0017767E-1016.70967737
GGTAATA8000.016.65000225
GGTACTT1901.8189894E-1216.5526312
Produced by FastQC (version 0.11.5)