##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632463.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758398
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0009744224009	34.0	33.0	34.0	31.0	34.0
2	33.14205469951134	34.0	33.0	34.0	31.0	34.0
3	33.21846312885846	34.0	33.0	34.0	31.0	34.0
4	36.51768332722396	37.0	37.0	37.0	35.0	37.0
5	36.477660806067526	37.0	37.0	37.0	35.0	37.0
6	36.52156519400104	37.0	37.0	37.0	35.0	37.0
7	36.52558287337256	37.0	37.0	37.0	35.0	37.0
8	36.49804719949156	37.0	37.0	37.0	35.0	37.0
9	38.287337255636224	39.0	39.0	39.0	37.0	39.0
10	38.30818119246095	39.0	39.0	39.0	37.0	39.0
11	38.380437448410994	39.0	39.0	39.0	37.0	39.0
12	38.337088177975154	39.0	39.0	39.0	37.0	39.0
13	38.372756784696165	39.0	39.0	39.0	37.0	39.0
14	39.914585745215575	41.0	40.0	41.0	38.0	41.0
15	39.91047049174708	41.0	40.0	41.0	38.0	41.0
16	39.86863230124552	41.0	40.0	41.0	38.0	41.0
17	39.86832375612805	41.0	40.0	41.0	38.0	41.0
18	39.871828512205994	41.0	40.0	41.0	38.0	41.0
19	39.874076672143126	41.0	40.0	41.0	38.0	41.0
20	39.84357421828644	41.0	40.0	41.0	38.0	41.0
21	39.81569967220378	41.0	40.0	41.0	38.0	41.0
22	39.78445881977537	41.0	40.0	41.0	38.0	41.0
23	39.743862721156965	41.0	40.0	41.0	38.0	41.0
24	39.72564009926187	41.0	40.0	41.0	38.0	41.0
25	39.68367532614801	41.0	40.0	41.0	37.0	41.0
26	39.60124894844132	41.0	40.0	41.0	37.0	41.0
27	39.51395837014338	41.0	40.0	41.0	37.0	41.0
28	39.47440262236979	41.0	39.0	41.0	37.0	41.0
29	39.443024638778056	41.0	39.0	41.0	37.0	41.0
30	39.39219117139022	41.0	39.0	41.0	36.0	41.0
31	39.3413326511937	41.0	39.0	41.0	36.0	41.0
32	39.29204982080649	41.0	39.0	41.0	36.0	41.0
33	39.21898264499643	41.0	39.0	41.0	36.0	41.0
34	39.178572464589834	41.0	39.0	41.0	35.0	41.0
35	39.10410101292461	41.0	39.0	41.0	35.0	41.0
36	39.06008059092983	40.0	39.0	41.0	35.0	41.0
37	39.00271097761334	40.0	39.0	41.0	35.0	41.0
38	38.927238732169656	40.0	39.0	41.0	35.0	41.0
39	38.838641452113535	40.0	38.0	41.0	35.0	41.0
40	38.763475114649566	40.0	38.0	41.0	35.0	41.0
41	38.70641009074391	40.0	38.0	41.0	35.0	41.0
42	38.637284117310436	40.0	38.0	41.0	35.0	41.0
43	37.8999773206153	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	7.0
18	3.0
19	21.0
20	35.0
21	73.0
22	149.0
23	245.0
24	448.0
25	656.0
26	961.0
27	1287.0
28	1895.0
29	2552.0
30	3436.0
31	4485.0
32	6038.0
33	8010.0
34	11912.0
35	19021.0
36	30290.0
37	58001.0
38	157778.0
39	451093.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.180625476333006	16.237648306034565	12.304489199602319	28.27723701803011
2	21.550557886492317	18.783277382060607	31.776455106685408	27.889709624761664
3	21.6270348814211	19.311364217732642	28.1331174396557	30.928483461190563
4	16.088913736586857	15.437672567701918	33.0217115551465	35.45170214056472
5	18.019957858538657	32.15527994535851	32.29531196021087	17.52945023589197
6	35.11981835395135	32.712770867011784	15.058584015253205	17.10882676378366
7	30.819965242524372	26.946405449381462	19.45073694814596	22.782892359948207
8	27.524597902420627	30.452348239314976	19.63902858393614	22.384025274328256
9	27.96987861254908	12.916964443471635	17.460884654231684	41.6522722897476
10	19.77457746460302	22.92134209214687	29.271042381440882	28.033038061809236
11	39.58950313687536	19.27985042154647	18.490027663575063	22.64061877800311
12	23.20865825068104	23.11319386390787	26.421483179016825	27.256664706394268
13	35.1236422036978	17.468927924387987	20.394436694189594	27.012993177724624
14	24.36135116390075	19.510599975210905	22.55886750756199	33.56918135332636
15	29.7349149127503	23.64022584447744	19.68715634798615	26.937702894786113
16	26.255607214154047	23.48357986176124	22.22935714492918	28.031455779155536
17	27.066922644838197	23.08985519476581	21.80016297511333	28.043059185282658
18	26.96644769632831	21.452720075738597	22.988720961816885	28.59211126611621
19	28.4752860635181	22.18795408215739	22.691647393584898	26.64511246073961
20	29.689819857119875	20.796468345116946	22.485686934828415	27.028024862934764
21	27.751259892563006	21.87097012386636	21.83273162640197	28.54503835716866
22	28.493218600260022	22.62848794432475	21.2913272450613	27.586966210353932
23	28.569431881413188	21.839851898343614	22.08405085456449	27.506665365678707
24	28.192848609832833	21.59472994390808	22.078381008388735	28.134040437870354
25	28.49967958776263	21.857257007534304	22.405913517704423	27.237149886998647
26	28.137468716953368	22.499927478711708	22.1065983823797	27.256005421955226
27	27.39564186614416	21.794361272049766	23.551749872758105	27.25824698904797
28	26.752048396752105	22.76772881785026	21.996366024172005	28.483856761225635
29	27.719614239489026	23.15762963509925	22.027879820358176	27.094876305053546
30	27.302419046463733	21.76706689627346	23.45641734287274	27.47409671439007
31	28.06072800824897	21.923976592765275	22.40920993989963	27.606085459086128
32	25.961434497453844	21.928064156287334	22.873346184984666	29.23715516127416
33	26.544373798454107	21.50730882729121	24.029731091063002	27.918586283191676
34	26.71737003525853	21.317303051959524	23.91105989203558	28.054267020746366
35	26.18677791871814	22.532496130000343	24.30267484882608	26.978051102455442
36	26.53896766605397	21.044227437308642	24.905920110548816	27.510884786088567
37	26.54727464998589	20.504009767958248	24.976199831750613	27.97251575030525
38	25.55505156922882	19.92027932563113	26.26826547538364	28.25640362975641
39	24.98688023966308	19.260071888375233	27.466449014897194	28.286598857064497
40	24.78500734442865	19.978691926930185	28.342242463719575	26.894058264921583
41	23.126775123352118	19.487920590507887	29.17676470665798	28.20853957948201
42	21.606333350035207	20.477902104172216	29.968037890395284	27.947726655397297
43	20.950081619413552	20.969464581921365	29.417271670020227	28.663182128644856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	20.0
4	33.0
5	33.0
6	43.5
7	54.0
8	59.0
9	64.0
10	83.0
11	102.0
12	102.0
13	231.0
14	360.0
15	590.5
16	821.0
17	793.0
18	765.0
19	765.0
20	956.5
21	1148.0
22	970.5
23	793.0
24	919.0
25	1045.0
26	1045.0
27	1300.0
28	1555.0
29	2020.5
30	2486.0
31	3381.5
32	4277.0
33	4277.0
34	5863.5
35	7450.0
36	8744.0
37	10038.0
38	13280.5
39	16523.0
40	16523.0
41	20013.5
42	23504.0
43	28067.5
44	32631.0
45	42546.5
46	52462.0
47	52462.0
48	59976.0
49	67490.0
50	74246.0
51	81002.0
52	83047.0
53	85092.0
54	85092.0
55	80592.5
56	76093.0
57	72729.0
58	69365.0
59	65269.0
60	61173.0
61	61173.0
62	56110.0
63	51047.0
64	43038.0
65	35029.0
66	30446.5
67	25864.0
68	25864.0
69	22311.5
70	18759.0
71	15736.5
72	12714.0
73	10161.0
74	7608.0
75	7608.0
76	6230.0
77	4852.0
78	3790.0
79	2728.0
80	2201.5
81	1675.0
82	1675.0
83	1297.5
84	920.0
85	683.0
86	446.0
87	352.5
88	259.0
89	259.0
90	178.5
91	98.0
92	72.0
93	46.0
94	30.5
95	15.0
96	15.0
97	8.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	758398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.28296114681282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6822379021078	46.18686314256795
2	6.992163139332039	7.451263137704116
3	2.0510585324787822	3.278594162877176
4	0.9667510009788	2.060454240951832
5	0.623052528527367	1.6599041834973591
6	0.40860586794574616	1.3063038351667753
7	0.3014987059579032	1.1245320684758506
8	0.22742564029229403	0.9694329244386666
9	0.1828311049058861	0.8767604393216271
>10	1.2890049496543667	13.747254647261995
>50	0.15110599157472435	5.590274495073029
>100	0.11308081898247005	11.391488736443875
>500	0.00994117089950191	3.4796990284762566
>1k	0.0012426463624377387	0.8771749577435296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1837	0.24222110290375237	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1300	0.17141395415072297	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1287	0.16969981460921574	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1122	0.14794342812085476	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1078	0.14214172505729183	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	943	0.12434104520317828	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	930	0.12262690566167106	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	929	0.1224950487738628	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	913	0.12038533856893084	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	865	0.11405620795413489	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	851	0.11221021152481943	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	819	0.10799079111495546	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	819	0.10799079111495546	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	803	0.1058810809100235	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	798	0.10522179647098225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6371377561649686E-4
2	0.0	0.0	0.0	0.0	2.6371377561649686E-4
3	0.0	0.0	0.0	0.0	2.6371377561649686E-4
4	0.0	0.0	0.0	0.0	2.6371377561649686E-4
5	0.0	0.0	0.0	0.0	2.6371377561649686E-4
6	0.0	0.0	0.0	0.0	2.6371377561649686E-4
7	0.0	0.0	0.0	0.0	2.6371377561649686E-4
8	2.6371377561649686E-4	0.0	0.0	0.0	2.6371377561649686E-4
9	2.6371377561649686E-4	0.0	0.0	0.0	2.6371377561649686E-4
10	2.6371377561649686E-4	0.0	0.0	0.0	2.6371377561649686E-4
11	2.6371377561649686E-4	0.0	0.0	0.0	2.6371377561649686E-4
12	2.6371377561649686E-4	0.0	0.0	0.0	3.955706634247453E-4
13	2.6371377561649686E-4	0.0	0.0	0.0	3.955706634247453E-4
14	2.6371377561649686E-4	0.0	0.0	1.3185688780824843E-4	3.955706634247453E-4
15	2.6371377561649686E-4	0.0	0.0	3.955706634247453E-4	3.955706634247453E-4
16	2.6371377561649686E-4	0.0	0.0	9.22998214657739E-4	3.955706634247453E-4
17	2.6371377561649686E-4	0.0	0.0	0.0015822826536989812	3.955706634247453E-4
18	2.6371377561649686E-4	0.0	0.0	0.0015822826536989812	3.955706634247453E-4
19	2.6371377561649686E-4	0.0	0.0	0.0019778533171237265	3.955706634247453E-4
20	2.6371377561649686E-4	0.0	0.0	0.002109710204931975	3.955706634247453E-4
21	2.6371377561649686E-4	0.0	0.0	0.0035601359708227077	3.955706634247453E-4
22	2.6371377561649686E-4	0.0	0.0	0.006988415053837167	3.955706634247453E-4
23	2.6371377561649686E-4	0.0	0.0	0.008570697707536148	3.955706634247453E-4
24	2.6371377561649686E-4	0.0	0.0	0.010548551024659875	3.955706634247453E-4
25	2.6371377561649686E-4	0.0	0.0	0.012526404341783601	3.955706634247453E-4
26	2.6371377561649686E-4	0.0	0.0	0.01621839720041456	3.955706634247453E-4
27	2.6371377561649686E-4	0.0	0.0	0.04707290894754469	3.955706634247453E-4
28	2.6371377561649686E-4	0.0	0.0	0.1787979398679849	3.955706634247453E-4
29	2.6371377561649686E-4	0.0	0.0	0.3715727098436441	3.955706634247453E-4
30	2.6371377561649686E-4	0.0	0.0	0.6402970471968544	3.955706634247453E-4
31	2.6371377561649686E-4	0.0	0.0	1.3871344597427735	3.955706634247453E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	28.859999	1
TCTTGTA	40	0.0019305847	23.125	2
TATACAC	310	0.0	22.677418	37
GATATGT	45	0.0038246263	20.555555	1
GAAATGA	55	5.140875E-4	20.181818	16
ATACGGC	85	1.2441651E-6	19.588236	29
CTTATAC	1045	0.0	19.119617	37
GTATCAA	1505	0.0	18.684387	2
GCGGTAA	705	0.0	18.631207	23
ACTATAT	50	0.0070327288	18.5	3
GCCATAG	60	9.232867E-4	18.5	1
TCTTATA	2055	0.0	18.004866	37
CTCTATG	225	0.0	17.266666	1
CGGTAAT	765	0.0	17.169935	24
GTACTAT	65	0.001579264	17.076923	1
AGTCGGT	175	7.2759576E-12	16.914286	11
CGCGGTA	800	0.0	16.88125	22
ATACACA	155	4.0017767E-10	16.709677	37
GGTAATA	800	0.0	16.650002	25
GGTACTT	190	1.8189894E-12	16.552631	2
>>END_MODULE