FastQCFastQC Report
Fri 10 Feb 2017
ERR1632462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164870
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4550.2759750106144235No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC3700.22441923940074No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG3590.2177473160672045No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG3570.21653423909747072No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC3400.20622308485473403No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC3030.18378116091466004No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2950.17892885303572512No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA2950.17892885303572512No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG2880.17468308364165705No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT2810.170437314247589No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG2740.16619154485352094No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA2700.1637653909140535No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2570.15588039061078424No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA2470.1498150057621156No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT2400.14556923636804756RNA PCR Primer, Index 29 (95% over 23bp)
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG2350.14253654394371323No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG2340.14193000545884635No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG2310.14011039000424577No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC2270.1376842360647783No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT2210.1340450051555771No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC2180.13222538970097653No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT2180.13222538970097653No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2160.1310123127312428No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA2160.1310123127312428No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG2100.12737308182204163No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2100.12737308182204163No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA2080.12616000485230788No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA1970.11948808151877237No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1960.1188815430339055No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1960.1188815430339055No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1950.11827500454903864No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC1930.1170619275793049No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA1920.11645538909443803No Hit
CTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCCGAACCT1900.1152423121247043No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC1890.11463577363983746No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT1850.11220961970037No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA1800.10917692727603566No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC1790.10857038879116879No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1790.10857038879116879No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1750.10614423485170134No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1720.10432461939710075No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT1720.10432461939710075No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA1690.10250500394250014No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1680.10189846545763329No Hit
CCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTCCTGGCCCTG1660.10068538848789957No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG1650.10007885000303268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA308.268837E-637.01
ATTAGAG352.3760083E-531.7142873
TATTAGA352.3760083E-531.7142872
AGGCACC303.586236E-430.8333328
TCTATGG303.586236E-430.8333322
GTATTAG502.6969428E-729.5999981
TAGTGAA250.005482865529.59999831
GGTATCA1450.029.3448281
CTAAGTA405.9097845E-527.7534
TATCTGT358.837071E-426.42857236
ACGGAGC706.504706E-926.42857231
CTCTATG358.837071E-426.4285721
AGTACCC358.837071E-426.42857237
AGCCGCG1750.026.4285719
GCGGTAA1650.025.78787823
CGGTAAT1700.025.02941124
GACACAT451.3165067E-424.66666826
TAAGTAC451.3165067E-424.66666835
CGGAGCT701.9022082E-723.78571532
CGCGGTA1800.023.6388922
Produced by FastQC (version 0.11.5)