FastQCFastQC Report
Fri 10 Feb 2017
ERR1632461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366353
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17020.4645792446083422No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11990.3272799731406594No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10170.2776011114962891No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8360.22819521063018455No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7500.20472058369932822No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6950.18970774089471085No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6780.18506740766419272No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6500.17742450587275113No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6380.1741489765335619No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6360.17360305497703035No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6180.16868976096824648No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5910.16131981995507066No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5890.16077389839853912No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5880.16050093762027334No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5790.1580442906158814No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5660.15449580049842637No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5620.1534039573853633No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5590.15258507505056598No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5450.14876362415484518No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5440.14849066337657943No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5430.14821770259831366No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5330.14548809481565594No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC5310.1449421732591244No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5080.13866407535901168No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5030.1372992714676828No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4900.13375078135022778No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4850.13238597745889893No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4820.1315670951241016No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4760.129929330454507No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4740.12938340889797545No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4700.12829156578491235No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4680.12774564422838083No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4660.12719972267184929No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4630.12638084033705196No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4570.12474307566745733No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4480.12228642866306541No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4430.12092162477173654No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4350.11873793854561038No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4340.11846497776734462No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT4330.11819201698907884TruSeq Adapter, Index 8 (95% over 22bp)
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4260.11628129154121844No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4110.11218687986723189No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4090.11164095831070033No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4090.11164095831070033No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4060.11082207597590303No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4040.11027615441937148No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3960.1080924681932453No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3940.10754654663671377No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3920.10700062508018224No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3910.10672766430191646No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3810.10399805651925874No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3720.10154140951486681No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3700.10099548795833527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTC250.00549151729.68
GTGCCCC250.00549151729.611
GGTATCA6200.028.645161
TCACTAC358.857818E-426.428572
CTAAGTC451.3208097E-424.66666634
GCAGTCG701.9154868E-723.7857139
CTCCCAT502.6972356E-422.21
CTTATTG753.7263817E-722.228
ATACGGC753.7263817E-722.229
GATTCCT753.7263817E-722.218
TGCGGGT4200.021.14285721
CAGTCGG806.9335147E-720.812510
CTATAGT450.00382090920.55555536
TAGAACA450.00382090920.5555554
CGTTAGA450.00382090920.5555551
CGCGGTA6200.020.29032122
GCCTTAT555.133906E-420.18181826
TCACAAT555.133906E-420.18181833
TAAGTCT555.133906E-420.18181835
CTTAGGA555.133906E-420.1818182
Produced by FastQC (version 0.11.5)