##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632461.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366353
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96997977360633	34.0	33.0	34.0	31.0	34.0
2	33.112549917702324	34.0	33.0	34.0	31.0	34.0
3	33.18730022683041	34.0	33.0	34.0	31.0	34.0
4	36.48878540642495	37.0	37.0	37.0	35.0	37.0
5	36.458325713178276	37.0	37.0	37.0	35.0	37.0
6	36.50093489066556	37.0	37.0	37.0	35.0	37.0
7	36.50962596184554	37.0	37.0	37.0	35.0	37.0
8	36.48342991595538	37.0	37.0	37.0	35.0	37.0
9	38.27253768905946	39.0	39.0	39.0	37.0	39.0
10	38.285102073683035	39.0	39.0	39.0	37.0	39.0
11	38.35968587673637	39.0	39.0	39.0	37.0	39.0
12	38.30970129902035	39.0	39.0	39.0	37.0	39.0
13	38.35507283958368	39.0	39.0	39.0	37.0	39.0
14	39.85573749907876	41.0	40.0	41.0	38.0	41.0
15	39.85691941924865	41.0	40.0	41.0	38.0	41.0
16	39.8039322729717	41.0	40.0	41.0	38.0	41.0
17	39.8139581223574	41.0	40.0	41.0	38.0	41.0
18	39.828343155372	41.0	40.0	41.0	38.0	41.0
19	39.819428256353845	41.0	40.0	41.0	38.0	41.0
20	39.799226429154395	41.0	40.0	41.0	38.0	41.0
21	39.74970315515364	41.0	40.0	41.0	38.0	41.0
22	39.71809429703046	41.0	40.0	41.0	38.0	41.0
23	39.67399202408606	41.0	40.0	41.0	38.0	41.0
24	39.655307858813764	41.0	40.0	41.0	37.0	41.0
25	39.618239785125276	41.0	40.0	41.0	37.0	41.0
26	39.51734802226268	41.0	40.0	41.0	37.0	41.0
27	39.39710606982883	41.0	39.0	41.0	36.0	41.0
28	39.368491591443224	41.0	39.0	41.0	36.0	41.0
29	39.31775910119475	41.0	39.0	41.0	36.0	41.0
30	39.27306450336151	41.0	39.0	41.0	36.0	41.0
31	39.19914672460714	41.0	39.0	41.0	35.0	41.0
32	39.140249977480735	41.0	39.0	41.0	35.0	41.0
33	39.070437528831484	40.0	39.0	41.0	35.0	41.0
34	39.028155904278115	40.0	39.0	41.0	35.0	41.0
35	38.94253356735171	40.0	39.0	41.0	35.0	41.0
36	38.88027121382928	40.0	39.0	41.0	35.0	41.0
37	38.81334669021408	40.0	38.0	41.0	35.0	41.0
38	38.726583377234526	40.0	38.0	41.0	35.0	41.0
39	38.60885266396072	40.0	38.0	41.0	35.0	41.0
40	38.52868954259962	40.0	38.0	41.0	35.0	41.0
41	38.45184016508668	40.0	38.0	41.0	35.0	41.0
42	38.378460119065494	40.0	37.0	41.0	35.0	41.0
43	37.62428586636386	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	2.0
16	0.0
17	3.0
18	5.0
19	18.0
20	22.0
21	41.0
22	81.0
23	159.0
24	254.0
25	391.0
26	531.0
27	648.0
28	1030.0
29	1379.0
30	1782.0
31	2434.0
32	3159.0
33	4095.0
34	6307.0
35	10106.0
36	15995.0
37	31215.0
38	79906.0
39	206788.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.23726023807639	16.1620076811163	12.637538112148667	27.963193968658643
2	22.44611071835088	19.768365483563667	30.87377474730656	26.911749050778894
3	22.32109468190516	18.97923587359732	28.74113218671609	29.95853725778143
4	15.999050096491635	16.316776442392992	34.02838246172408	33.6557909993913
5	18.136060029534356	32.0081997417791	31.673276866847004	18.182463361839538
6	32.67531588386065	33.63368117635177	15.794329512792308	17.896673426995275
7	29.604780089148992	27.379057903169894	19.001072735858585	24.015089271822532
8	26.845146620882044	29.597137187357546	20.447491899888906	23.1102242918715
9	28.027612712329365	12.688035856127833	17.361670301594366	41.92268112994844
10	20.41282588104915	22.39069968036293	27.922249851918778	29.27422458666914
11	39.70815033587824	19.428802275401047	18.363436357829745	22.49961103089097
12	22.54928989253534	23.439142029681754	26.32843186762494	27.68313621015796
13	37.05660933580454	17.11928113049436	19.54044323371175	26.283666299989356
14	24.277404579735936	20.184084748862436	22.687681007116087	32.85082966428554
15	30.59617363581027	23.29938611120968	19.32780678744271	26.776633465537337
16	25.132590698042602	23.784437414187956	22.53236632428286	28.55060556348658
17	27.28679716011606	23.207398328934115	21.085128277917747	28.420676233032076
18	27.362953217252212	20.192000611432142	22.775574377717668	29.669471793597978
19	29.10198633558344	22.121014431436347	22.499065109334442	26.277934123645775
20	30.855486375162755	20.044601791168624	21.80437992864805	27.29553190502057
21	28.05354398626461	21.186396726654348	21.6070292859619	29.15303000111914
22	28.47717911413309	22.638002145471717	20.663130914718863	28.221687825676327
23	29.154940726567	21.121977982983626	21.686733833215506	28.036347457233866
24	28.26618043253365	20.68223816919747	22.35248517140572	28.699096226863162
25	29.168588765480287	21.684277186211112	22.1046367847404	27.0424972635682
26	28.955952319211253	22.181065802654818	21.75606587089501	27.10691600723892
27	27.361042491804355	21.656162226049737	24.020002565831316	26.962792716314592
28	27.1126481835825	23.097941056849542	21.03708718094297	28.75232357862499
29	27.478142665680366	23.608923633763066	21.423599642967304	27.489334057589264
30	27.305358493038135	21.63296055989715	23.965137449399894	27.096543497664822
31	28.885528438418685	21.770805752921362	21.6684454610717	27.675220347588255
32	25.82427331016806	21.546977914743433	22.67840034065505	29.950348434433455
33	26.592930861764476	21.248358823320675	23.932655116786268	28.22605519812858
34	26.414414512778663	21.491020955198948	23.52703540028333	28.56752913173906
35	26.19577292938778	23.053721410770486	23.927741822777485	26.82276383706425
36	26.804475464920447	21.141358198240496	24.735159804887637	27.319006531951423
37	27.34957813911719	20.7234552467156	23.9842447038785	27.94272191028871
38	25.911347798434843	20.051698771403537	25.75548719404509	28.281466236116533
39	25.33949496796805	19.182591653405325	27.014655264185087	28.463258114441537
40	25.25078271503168	20.017578674120315	28.000589595281056	26.731049015566953
41	23.39874383449842	19.614961526178305	28.785897754351673	28.200396884971596
42	21.453898289354804	20.630648582105238	29.920322748824223	27.99513037971574
43	20.58397228902179	21.456900857915727	28.76242312742082	29.196703725641665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.5
2	8.0
3	16.5
4	25.0
5	25.0
6	26.0
7	27.0
8	31.0
9	35.0
10	55.0
11	75.0
12	75.0
13	149.5
14	224.0
15	375.5
16	527.0
17	502.5
18	478.0
19	478.0
20	598.0
21	718.0
22	606.5
23	495.0
24	562.5
25	630.0
26	630.0
27	740.5
28	851.0
29	1064.0
30	1277.0
31	1485.0
32	1693.0
33	1693.0
34	2381.5
35	3070.0
36	3213.5
37	3357.0
38	4326.0
39	5295.0
40	5295.0
41	7062.5
42	8830.0
43	11204.5
44	13579.0
45	19739.0
46	25899.0
47	25899.0
48	29949.0
49	33999.0
50	37704.0
51	41409.0
52	42730.0
53	44051.0
54	44051.0
55	40922.5
56	37794.0
57	36139.0
58	34484.0
59	32350.5
60	30217.0
61	30217.0
62	27886.5
63	25556.0
64	20606.0
65	15656.0
66	13735.0
67	11814.0
68	11814.0
69	10057.0
70	8300.0
71	6996.0
72	5692.0
73	4621.0
74	3550.0
75	3550.0
76	2926.5
77	2303.0
78	1935.0
79	1567.0
80	1350.0
81	1133.0
82	1133.0
83	996.0
84	859.0
85	624.5
86	390.0
87	343.0
88	296.0
89	296.0
90	205.0
91	114.0
92	82.0
93	50.0
94	35.0
95	20.0
96	20.0
97	11.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	366353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.91995258532652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.70828239917809	39.357202639400334
2	6.948617379253652	6.381603611778072
3	2.1584940658797387	2.9735383548271885
4	1.1259293478289418	2.068104890669305
5	0.6586819688092526	1.5123322388265203
6	0.4862386213054141	1.3396832661299491
7	0.36105565459495564	1.1605760977765076
8	0.27472228551132216	1.0092187459849962
9	0.20031944526179693	0.8278793485506565
>10	1.6743362343138217	15.598083429937853
>50	0.21271532579325114	6.811997163686907
>100	0.1756693982674602	16.25144367040623
>500	0.013145329122054846	3.6333212406469575
>1k	0.001792544880280206	1.0750153013785517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1702	0.4645792446083422	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1199	0.3272799731406594	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1017	0.2776011114962891	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	836	0.22819521063018455	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	750	0.20472058369932822	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	695	0.18970774089471085	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	678	0.18506740766419272	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	650	0.17742450587275113	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	638	0.1741489765335619	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	636	0.17360305497703035	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	618	0.16868976096824648	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	591	0.16131981995507066	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	589	0.16077389839853912	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	588	0.16050093762027334	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	579	0.1580442906158814	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	566	0.15449580049842637	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	562	0.1534039573853633	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	559	0.15258507505056598	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	545	0.14876362415484518	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	544	0.14849066337657943	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	543	0.14821770259831366	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	533	0.14548809481565594	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	531	0.1449421732591244	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	508	0.13866407535901168	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	503	0.1372992714676828	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	490	0.13375078135022778	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	485	0.13238597745889893	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	482	0.1315670951241016	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	476	0.129929330454507	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	474	0.12938340889797545	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	470	0.12829156578491235	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	468	0.12774564422838083	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	466	0.12719972267184929	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	463	0.12638084033705196	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	457	0.12474307566745733	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	448	0.12228642866306541	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	443	0.12092162477173654	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	435	0.11873793854561038	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	434	0.11846497776734462	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	433	0.11819201698907884	TruSeq Adapter, Index 8 (95% over 22bp)
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	426	0.11628129154121844	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	411	0.11218687986723189	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	409	0.11164095831070033	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	409	0.11164095831070033	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	406	0.11082207597590303	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	404	0.11027615441937148	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	396	0.1080924681932453	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	394	0.10754654663671377	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	392	0.10700062508018224	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	391	0.10672766430191646	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	381	0.10399805651925874	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	372	0.10154140951486681	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	370	0.10099548795833527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.72960778265771E-4
6	0.0	0.0	0.0	0.0	2.72960778265771E-4
7	0.0	0.0	0.0	0.0	2.72960778265771E-4
8	0.0	0.0	0.0	0.0	2.72960778265771E-4
9	0.0	0.0	0.0	0.0	2.72960778265771E-4
10	0.0	0.0	0.0	0.0	2.72960778265771E-4
11	0.0	0.0	0.0	0.0	2.72960778265771E-4
12	0.0	0.0	0.0	0.0	2.72960778265771E-4
13	0.0	0.0	0.0	0.0	2.72960778265771E-4
14	0.0	0.0	0.0	0.0	2.72960778265771E-4
15	0.0	0.0	0.0	0.0	2.72960778265771E-4
16	0.0	0.0	0.0	5.45921556531542E-4	2.72960778265771E-4
17	0.0	0.0	0.0	8.18882334797313E-4	2.72960778265771E-4
18	0.0	0.0	0.0	8.18882334797313E-4	2.72960778265771E-4
19	0.0	0.0	0.0	0.001091843113063084	2.72960778265771E-4
20	0.0	0.0	0.0	0.001091843113063084	2.72960778265771E-4
21	2.72960778265771E-4	0.0	0.0	0.002183686226126168	2.72960778265771E-4
22	2.72960778265771E-4	0.0	0.0	0.004367372452252336	2.72960778265771E-4
23	2.72960778265771E-4	0.0	0.0	0.004913294008783878	2.72960778265771E-4
24	2.72960778265771E-4	0.0	0.0	0.008188823347973129	2.72960778265771E-4
25	2.72960778265771E-4	0.0	0.0	0.011737313465428152	2.72960778265771E-4
26	2.72960778265771E-4	0.0	0.0	0.018015411365540884	2.72960778265771E-4
27	2.72960778265771E-4	0.0	0.0	0.055956959544483056	2.72960778265771E-4
28	2.72960778265771E-4	0.0	0.0	0.19216438789910278	2.72960778265771E-4
29	2.72960778265771E-4	0.0	0.0	0.3829639719068767	2.72960778265771E-4
30	2.72960778265771E-4	0.0	0.0	0.6602921226249	2.72960778265771E-4
31	2.72960778265771E-4	0.0	0.0	1.3997428709468736	2.72960778265771E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTC	25	0.005491517	29.6	8
GTGCCCC	25	0.005491517	29.6	11
GGTATCA	620	0.0	28.64516	1
TCACTAC	35	8.857818E-4	26.42857	2
CTAAGTC	45	1.3208097E-4	24.666666	34
GCAGTCG	70	1.9154868E-7	23.785713	9
CTCCCAT	50	2.6972356E-4	22.2	1
CTTATTG	75	3.7263817E-7	22.2	28
ATACGGC	75	3.7263817E-7	22.2	29
GATTCCT	75	3.7263817E-7	22.2	18
TGCGGGT	420	0.0	21.142857	21
CAGTCGG	80	6.9335147E-7	20.8125	10
CTATAGT	45	0.003820909	20.555555	36
TAGAACA	45	0.003820909	20.555555	4
CGTTAGA	45	0.003820909	20.555555	1
CGCGGTA	620	0.0	20.290321	22
GCCTTAT	55	5.133906E-4	20.181818	26
TCACAAT	55	5.133906E-4	20.181818	33
TAAGTCT	55	5.133906E-4	20.181818	35
CTTAGGA	55	5.133906E-4	20.181818	2
>>END_MODULE