FastQCFastQC Report
Fri 10 Feb 2017
ERR1632460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664041
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26480.39877055784206095No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19450.29290360083187633No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16660.2508881228719311No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15150.22814856311583173No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14700.22137187312229215No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12370.18628367826685402No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12360.18613308515588647No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12280.18492834026814609No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12090.18206707115976273No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11590.17453741561138544No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11570.17423622938945035No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10920.1644476771765599No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10690.16098403562430633No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10370.15616505607334488No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC10330.15556268362947467No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC10260.15450853185270186No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10190.15345438007592904No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10040.15119548341141587No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9540.14366582786303858No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC8900.13402786876111564No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8880.13372668253918057No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8770.13207015831853755No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8750.13176897209660246No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8740.13161837898563492No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8610.12966066854305683No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8540.128606516766284No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8260.12438990965919274No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8230.1239381303262901No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7810.11761321966565316No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7740.11655906788888035No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7710.11610728855597771No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7550.11369779878049699No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7520.11324601944759434No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7350.11068593656114607No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7340.11053534345017854No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7290.1097823778953408No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7150.10767407434179516No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7110.10707170189792498No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7100.10692110878695743No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6990.10526458456631443No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6910.10405983967857406No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6860.10330687412373635No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC6850.1031562810127688No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6830.1028550947908337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT200.001841376137.033
ACTTATA250.005494693329.59999831
TATCTAT250.005494693329.5999987
GGTATCA12450.028.232931
AAGACTA405.9364087E-527.75000215
GAGCTCT358.8654354E-426.4285737
AGAGGGT358.8654354E-426.428576
CTAAGTC509.083542E-625.89999834
ATCTTAC809.767973E-1025.4375021
ATACGGC2050.025.26829329
CTTAAAC601.3359859E-624.6666683
TAAACCG451.3223897E-424.6666685
TAAGTCT601.3359859E-624.66666835
ATTGACT451.3223897E-424.66666831
CTAATCG451.3223897E-424.66666819
GCTTAAA551.9010902E-523.5454542
TTTAGAG400.001930332723.1250023
GTTTCAA400.001930332723.12500222
CCGACTA400.001930332723.1250029
TTGTCTG502.7004376E-422.1999999
Produced by FastQC (version 0.11.5)