##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664041
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0254321645802	34.0	33.0	34.0	31.0	34.0
2	33.16635870375474	34.0	33.0	34.0	31.0	34.0
3	33.23708777018287	34.0	33.0	34.0	31.0	34.0
4	36.517593040188785	37.0	37.0	37.0	35.0	37.0
5	36.49404931322012	37.0	37.0	37.0	35.0	37.0
6	36.542464998396184	37.0	37.0	37.0	35.0	37.0
7	36.5507792440527	37.0	37.0	37.0	35.0	37.0
8	36.52715871459744	37.0	37.0	37.0	35.0	37.0
9	38.32100578126953	39.0	39.0	39.0	37.0	39.0
10	38.35136234057837	39.0	39.0	39.0	37.0	39.0
11	38.42301604870784	39.0	39.0	39.0	37.0	39.0
12	38.37919495934739	39.0	39.0	39.0	37.0	39.0
13	38.41614147319217	39.0	39.0	39.0	37.0	39.0
14	39.945637392871824	41.0	40.0	41.0	38.0	41.0
15	39.95150751233734	41.0	40.0	41.0	38.0	41.0
16	39.9001853801196	41.0	40.0	41.0	38.0	41.0
17	39.90944233865078	41.0	40.0	41.0	38.0	41.0
18	39.92563410994201	41.0	40.0	41.0	38.0	41.0
19	39.92373964860604	41.0	40.0	41.0	38.0	41.0
20	39.90296532894806	41.0	40.0	41.0	38.0	41.0
21	39.86478997531779	41.0	40.0	41.0	38.0	41.0
22	39.83541227123024	41.0	40.0	41.0	38.0	41.0
23	39.80690047753075	41.0	40.0	41.0	38.0	41.0
24	39.78500423919608	41.0	40.0	41.0	38.0	41.0
25	39.742785460536325	41.0	40.0	41.0	38.0	41.0
26	39.65278047590435	41.0	40.0	41.0	37.0	41.0
27	39.548136334955224	41.0	40.0	41.0	37.0	41.0
28	39.51798307634619	41.0	40.0	41.0	37.0	41.0
29	39.4777506208201	41.0	39.0	41.0	37.0	41.0
30	39.43442498279474	41.0	39.0	41.0	37.0	41.0
31	39.37047863008459	41.0	39.0	41.0	36.0	41.0
32	39.32630214098226	41.0	39.0	41.0	36.0	41.0
33	39.265363132698134	41.0	39.0	41.0	36.0	41.0
34	39.22179202790189	41.0	39.0	41.0	35.0	41.0
35	39.13361524363707	41.0	39.0	41.0	35.0	41.0
36	39.08662567522186	41.0	39.0	41.0	35.0	41.0
37	39.027626306207	41.0	39.0	41.0	35.0	41.0
38	38.95006784219649	40.0	39.0	41.0	35.0	41.0
39	38.845355934347424	40.0	38.0	41.0	35.0	41.0
40	38.75996060484217	40.0	38.0	41.0	35.0	41.0
41	38.69723104446864	40.0	38.0	41.0	35.0	41.0
42	38.62237873866222	40.0	38.0	41.0	35.0	41.0
43	37.884496589819	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	7.0
19	12.0
20	19.0
21	68.0
22	125.0
23	255.0
24	366.0
25	592.0
26	759.0
27	1054.0
28	1538.0
29	2132.0
30	2845.0
31	3736.0
32	4991.0
33	6790.0
34	10527.0
35	16179.0
36	25201.0
37	49671.0
38	135268.0
39	401904.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.532915889229734	16.66629018388925	12.559465454693308	28.24132847218771
2	22.221971233703943	19.101681974456397	32.031305295907934	26.645041495931725
3	21.624568362495687	19.619872869295722	29.030135187435718	29.725423580772876
4	15.542413796738455	16.50470377582107	35.18141199112706	32.77147043631342
5	17.611563141432534	32.16051418511809	32.85595317156621	17.371969501883168
6	31.676507926468396	34.73475282399731	15.953984769012758	17.634754480521533
7	29.275752551423786	27.379634691231413	19.873923447497972	23.470689309846833
8	25.96180055147197	30.223284405631578	20.823413012148347	22.9915020307481
9	28.257291341950268	12.60494457420551	18.16514341734923	40.97262066649499
10	20.158243241004698	22.15149365777113	28.644014450914927	29.04624865030924
11	39.2088741508431	19.59412144732027	18.821879974278698	22.37512442755794
12	22.515778393201625	23.98059758358294	26.331506638897295	27.17211738431814
13	35.52988444990595	17.61638212098349	20.32269091818126	26.531042510929293
14	23.73407666092907	20.64842381720406	23.083815607771207	32.53368391409567
15	30.443300940755165	23.582730584406686	20.024667151576484	25.949301323261665
16	24.99755286194678	23.91975796675205	23.19585688233106	27.88683228897011
17	27.029806894453806	23.59583218506086	21.490691086845544	27.88366983363979
18	26.21058037079036	21.175499705590468	23.396296313028863	29.21762361059031
19	28.241780251520616	22.346361143363136	23.27326174136838	26.13859686374787
20	30.146180732816198	20.21200498162011	22.879310163077278	26.762504122486412
21	27.15841341122009	22.04668085253772	22.262179594332277	28.532726141909915
22	28.31090248945472	23.116193126629227	21.113154157649905	27.45975022626615
23	28.510739547708653	21.602732361405394	22.434759299501085	27.45176879138487
24	28.092391885440808	21.577884498095752	22.44364429304817	27.88607932341527
25	28.439960785553904	21.98885309792618	22.94301104901655	26.628175067503363
26	28.177627586248438	23.064389096456395	22.313230658950275	26.44475265834489
27	27.08733346284341	22.0203270581184	24.531467183502222	26.36087229553597
28	26.20982740523552	23.506229284035175	22.070323970959624	28.213619339769686
29	27.56832785927375	23.854701742814076	22.27362467076581	26.303345727146365
30	26.664769193468473	21.528640550809364	24.75750744306451	27.049082812657655
31	28.077784353676954	22.31413421761608	22.668931587055617	26.939149841651343
32	25.115768454056305	21.836001090294126	23.34208279308055	29.706147662569027
33	25.956529792588107	21.454096960880428	24.84470085431472	27.744672392216746
34	26.173986244825244	21.42458071113079	24.71760026865811	27.68383277538586
35	25.89433483775851	23.528065285125464	24.524389307286746	26.05321056982927
36	26.196725804581344	21.344314582985085	25.478697851488086	26.980261760945485
37	26.587514927542127	20.981987557997172	24.943640528220396	27.48685698624031
38	25.679438468407824	20.092132865289944	26.42833800924943	27.8000906570528
39	24.90222742270432	19.276520576289716	27.99164509420352	27.82960690680244
40	24.74094220085808	20.592854959257036	28.460140262423554	26.20606257746133
41	22.86635915553407	19.46054535789206	29.607509174885287	28.065586311688584
42	20.7586579744323	21.1577297184963	30.429145188324213	27.654467118747185
43	20.395728577000515	21.55800620744803	29.574378690472425	28.471886525079025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	11.0
4	20.0
5	20.0
6	37.0
7	54.0
8	48.5
9	43.0
10	79.5
11	116.0
12	116.0
13	241.5
14	367.0
15	638.0
16	909.0
17	838.5
18	768.0
19	768.0
20	902.5
21	1037.0
22	709.0
23	381.0
24	366.5
25	352.0
26	352.0
27	438.0
28	524.0
29	774.0
30	1024.0
31	1575.5
32	2127.0
33	2127.0
34	3771.5
35	5416.0
36	6175.0
37	6934.0
38	10508.5
39	14083.0
40	14083.0
41	17269.0
42	20455.0
43	25515.0
44	30575.0
45	41344.5
46	52114.0
47	52114.0
48	59898.5
49	67683.0
50	75287.0
51	82891.0
52	83484.5
53	84078.0
54	84078.0
55	77241.5
56	70405.0
57	65678.5
58	60952.0
59	56323.0
60	51694.0
61	51694.0
62	45949.5
63	40205.0
64	31799.0
65	23393.0
66	19917.5
67	16442.0
68	16442.0
69	13758.5
70	11075.0
71	9216.0
72	7357.0
73	5825.5
74	4294.0
75	4294.0
76	3497.0
77	2700.0
78	2101.0
79	1502.0
80	1216.0
81	930.0
82	930.0
83	746.0
84	562.0
85	459.0
86	356.0
87	269.0
88	182.0
89	182.0
90	107.0
91	32.0
92	18.5
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	664041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.528267162532252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52307193851678	21.830706403596814
2	9.27518095580046	5.292096805758135
3	3.642925364200872	3.1177904412926267
4	2.0836849692579107	2.377756859421699
5	1.4276886615281619	2.0364741780496742
6	0.9205866167930753	1.575764457007473
7	0.7298276086200346	1.457450190091306
8	0.5573114806755448	1.2719304650766696
9	0.4464659888175265	1.146321090717348
>10	3.4936086128753097	20.19249769208485
>50	0.49177914138053974	9.75527341412833
>100	0.3696309745149569	20.833662979178673
>500	0.028678265264091486	5.513968761784527
>1k	0.009559421754697161	3.5983062618119064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2648	0.39877055784206095	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1945	0.29290360083187633	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1666	0.2508881228719311	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1515	0.22814856311583173	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1470	0.22137187312229215	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1237	0.18628367826685402	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1236	0.18613308515588647	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1228	0.18492834026814609	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1209	0.18206707115976273	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1159	0.17453741561138544	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1157	0.17423622938945035	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1092	0.1644476771765599	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1069	0.16098403562430633	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1037	0.15616505607334488	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	1033	0.15556268362947467	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1026	0.15450853185270186	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1019	0.15345438007592904	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1004	0.15119548341141587	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	954	0.14366582786303858	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	890	0.13402786876111564	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	888	0.13372668253918057	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	877	0.13207015831853755	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	875	0.13176897209660246	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	874	0.13161837898563492	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	861	0.12966066854305683	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	854	0.128606516766284	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	826	0.12438990965919274	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	823	0.1239381303262901	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	781	0.11761321966565316	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	774	0.11655906788888035	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	771	0.11610728855597771	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	755	0.11369779878049699	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	752	0.11324601944759434	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	735	0.11068593656114607	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	734	0.11053534345017854	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	729	0.1097823778953408	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	715	0.10767407434179516	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	711	0.10707170189792498	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	710	0.10692110878695743	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	699	0.10526458456631443	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	691	0.10405983967857406	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	686	0.10330687412373635	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	685	0.1031562810127688	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	683	0.1028550947908337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.0118622193509134E-4	0.0	0.0	0.0	0.0
9	3.0118622193509134E-4	0.0	0.0	0.0	1.5059311096754567E-4
10	3.0118622193509134E-4	0.0	0.0	0.0	1.5059311096754567E-4
11	3.0118622193509134E-4	0.0	0.0	0.0	1.5059311096754567E-4
12	3.0118622193509134E-4	0.0	0.0	1.5059311096754567E-4	1.5059311096754567E-4
13	3.0118622193509134E-4	0.0	0.0	1.5059311096754567E-4	1.5059311096754567E-4
14	3.0118622193509134E-4	0.0	0.0	1.5059311096754567E-4	1.5059311096754567E-4
15	3.0118622193509134E-4	0.0	0.0	1.5059311096754567E-4	1.5059311096754567E-4
16	3.0118622193509134E-4	0.0	0.0	6.023724438701827E-4	1.5059311096754567E-4
17	4.51779332902637E-4	0.0	0.0	7.529655548377284E-4	1.5059311096754567E-4
18	4.51779332902637E-4	0.0	0.0	7.529655548377284E-4	1.5059311096754567E-4
19	4.51779332902637E-4	0.0	0.0	0.0012047448877403654	1.5059311096754567E-4
20	4.51779332902637E-4	0.0	0.0	0.0021083035535456396	1.5059311096754567E-4
21	4.51779332902637E-4	0.0	0.0	0.004668386439993916	1.5059311096754567E-4
22	4.51779332902637E-4	0.0	0.0	0.007379062437409738	1.5059311096754567E-4
23	4.51779332902637E-4	0.0	0.0	0.008282621103215012	1.5059311096754567E-4
24	4.51779332902637E-4	0.0	0.0	0.010240331545793107	1.5059311096754567E-4
25	4.51779332902637E-4	0.0	0.0	0.01249922821030629	1.5059311096754567E-4
26	4.51779332902637E-4	0.0	0.0	0.020330069980618665	1.5059311096754567E-4
27	4.51779332902637E-4	0.0	0.0	0.058430127055407725	3.0118622193509134E-4
28	4.51779332902637E-4	0.0	0.0	0.19441570625910148	3.0118622193509134E-4
29	4.51779332902637E-4	0.0	0.0	0.40178242006141185	3.0118622193509134E-4
30	4.51779332902637E-4	0.0	0.0	0.6864033997900733	3.0118622193509134E-4
31	4.51779332902637E-4	0.0	0.0	1.4532235208368158	3.0118622193509134E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACT	20	0.0018413761	37.0	33
ACTTATA	25	0.0054946933	29.599998	31
TATCTAT	25	0.0054946933	29.599998	7
GGTATCA	1245	0.0	28.23293	1
AAGACTA	40	5.9364087E-5	27.750002	15
GAGCTCT	35	8.8654354E-4	26.42857	37
AGAGGGT	35	8.8654354E-4	26.42857	6
CTAAGTC	50	9.083542E-6	25.899998	34
ATCTTAC	80	9.767973E-10	25.437502	1
ATACGGC	205	0.0	25.268293	29
CTTAAAC	60	1.3359859E-6	24.666668	3
TAAACCG	45	1.3223897E-4	24.666668	5
TAAGTCT	60	1.3359859E-6	24.666668	35
ATTGACT	45	1.3223897E-4	24.666668	31
CTAATCG	45	1.3223897E-4	24.666668	19
GCTTAAA	55	1.9010902E-5	23.545454	2
TTTAGAG	40	0.0019303327	23.125002	3
GTTTCAA	40	0.0019303327	23.125002	22
CCGACTA	40	0.0019303327	23.125002	9
TTGTCTG	50	2.7004376E-4	22.199999	9
>>END_MODULE