FastQCFastQC Report
Fri 10 Feb 2017
ERR1632459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189184
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9460.5000422868741542No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7000.37001014884979705No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6700.3541525710419486No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5770.3049940798376184No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5210.27539326792963464No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4800.25372124492557513No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4760.251606901217862No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4090.21619164411366712No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3960.20932002706359945No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3930.20773426928281463No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3910.20667709742895804No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3860.20403416779431666No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3840.20297699594046006No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3840.20297699594046006No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3800.20086265223274694No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3760.19874830852503383No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3700.19557679296346417No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3580.18923376184032475No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3550.18764800405953994No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT3550.18764800405953994Illumina PCR Primer Index 10 (95% over 21bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3280.17337618403247632No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3250.17179042625169147No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3210.16967608254397834No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3100.16386163734776724No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3020.159632949932341No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3010.15910436400541272No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2990.15804719215155616No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2960.1564614343707713No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2960.1564614343707713No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2910.1538185047361299No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2820.14906123139377536No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2790.14747547361299052No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2750.1453611299052774No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2740.1448325439783491No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2730.14430395805142085No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2620.13848951285520975No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2600.13743234100135318No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2570.13584658322056833No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2430.1284463802435724No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2420.1279177943166441No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2410.12738920838971582No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2340.12368910690121786No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2330.12316052097428958No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2330.12316052097428958No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2320.1226319350473613No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2290.12104617726657646No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2280.12051759133964816No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2270.1199890054127199No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2260.11946041948579161No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2240.11840324763193505No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2230.11787466170500675No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2220.11734607577807848No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2220.11734607577807848No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2210.1168174898511502No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2170.11470314614343707No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2130.11258880243572396No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2120.11206021650879566No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2100.11100304465493911No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2060.10888870094722597No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2060.10888870094722597No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2050.1083601150202977No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2050.1083601150202977No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2030.10730294316644114No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2020.10677435723951285No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1990.10518859945872802No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1970.10413142760487144No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1960.10360284167794317No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT1940.10254566982408661No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1930.10201708389715833No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1920.10148849797023003No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1900.10043132611637347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT200.001838051437.01
TCAATGC200.001838051437.032
CAATGCT200.001838051437.033
ATACGGC308.275294E-637.029
TACGGCT352.3778504E-531.71428530
CGTTATG250.00548488529.62
AGACCCG250.00548488529.65
TGCAGGT250.00548488529.616
GGTATCA3050.028.5081961
AAGTCTG601.3275621E-624.66666836
TAACCCT451.3175112E-424.66666637
CATGGGA400.001925260223.12500226
ATGGGAG400.001925260223.12500227
TGGGAGT400.001925260223.12500228
CTAAGTC652.6632224E-622.7692334
TAAGTCT652.6632224E-622.7692335
GGTAATA3450.022.52173825
TAATACG3300.022.42424227
TATGCCG502.6905545E-422.233
GCGGTAA3650.021.2876723
Produced by FastQC (version 0.11.5)