##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189184
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99780108254398	34.0	33.0	34.0	31.0	34.0
2	33.13394367388363	34.0	33.0	34.0	31.0	34.0
3	33.20003805818674	34.0	33.0	34.0	31.0	34.0
4	36.49256808186739	37.0	37.0	37.0	35.0	37.0
5	36.462158533491206	37.0	37.0	37.0	35.0	37.0
6	36.50802922023004	37.0	37.0	37.0	35.0	37.0
7	36.51591572225981	37.0	37.0	37.0	35.0	37.0
8	36.493889546684706	37.0	37.0	37.0	35.0	37.0
9	38.285346540933695	39.0	39.0	39.0	37.0	39.0
10	38.32371659336942	39.0	39.0	39.0	37.0	39.0
11	38.389266534167795	39.0	39.0	39.0	37.0	39.0
12	38.33367515223275	39.0	39.0	39.0	37.0	39.0
13	38.37293851488498	39.0	39.0	39.0	37.0	39.0
14	39.889245390730714	41.0	40.0	41.0	38.0	41.0
15	39.90035098105548	41.0	40.0	41.0	38.0	41.0
16	39.84198452300406	41.0	40.0	41.0	38.0	41.0
17	39.851308778755076	41.0	40.0	41.0	38.0	41.0
18	39.86147877198918	41.0	40.0	41.0	38.0	41.0
19	39.85603433694181	41.0	40.0	41.0	38.0	41.0
20	39.84644050236807	41.0	40.0	41.0	38.0	41.0
21	39.79032053450609	41.0	40.0	41.0	38.0	41.0
22	39.75294422361299	41.0	40.0	41.0	38.0	41.0
23	39.70370115866035	41.0	40.0	41.0	38.0	41.0
24	39.681352545669824	41.0	40.0	41.0	37.0	41.0
25	39.631580894790254	41.0	40.0	41.0	37.0	41.0
26	39.52238032814614	41.0	40.0	41.0	37.0	41.0
27	39.3745348443843	41.0	39.0	41.0	36.0	41.0
28	39.35180036366712	41.0	39.0	41.0	36.0	41.0
29	39.30837702976996	41.0	39.0	41.0	36.0	41.0
30	39.23546388700947	41.0	39.0	41.0	36.0	41.0
31	39.15320005920162	41.0	39.0	41.0	35.0	41.0
32	39.09648807510149	40.0	39.0	41.0	35.0	41.0
33	39.00863180818674	40.0	39.0	41.0	35.0	41.0
34	38.953315290933695	40.0	39.0	41.0	35.0	41.0
35	38.85351298207036	40.0	38.0	41.0	35.0	41.0
36	38.76185089648173	40.0	38.0	41.0	35.0	41.0
37	38.67112440798376	40.0	38.0	41.0	35.0	41.0
38	38.56917075439784	40.0	38.0	41.0	35.0	41.0
39	38.43693441305819	40.0	37.0	41.0	35.0	41.0
40	38.32953103856563	40.0	37.0	41.0	35.0	41.0
41	38.25582501691475	40.0	37.0	41.0	35.0	41.0
42	38.1710662635318	40.0	37.0	41.0	34.0	41.0
43	37.40034040933694	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	3.0
20	7.0
21	21.0
22	42.0
23	93.0
24	136.0
25	189.0
26	281.0
27	355.0
28	496.0
29	715.0
30	903.0
31	1194.0
32	1637.0
33	2239.0
34	3212.0
35	5357.0
36	8553.0
37	17605.0
38	42597.0
39	103546.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.791758288227335	15.712216677943166	12.511628890392423	27.984396143437078
2	23.55378890392422	19.15542540595399	29.904220230040597	27.38656546008119
3	23.193821887686063	18.275858423545333	27.978053112313937	30.552266576454667
4	16.524124661705006	16.24291694857916	33.74756850473613	33.4853898849797
5	19.178154600811908	31.288058186738837	30.67754144113667	18.856245771312587
6	32.684053619756426	32.88703061569689	15.95378044654939	18.475135317997292
7	29.83497547361299	26.1121447902571	18.660668978349122	25.392210757780788
8	27.1307298714479	28.471223782138022	20.712110960757784	23.685935385656293
9	27.516597598105548	12.260021989174561	16.921621278755076	43.301759133964815
10	21.596963802435724	20.97323240866035	26.675617388362653	30.754186400541272
11	40.93580852503383	18.91597598105548	17.981964648173207	22.166250845737483
12	22.41944350473613	22.537846752368065	26.073029431664413	28.969680311231393
13	38.273321211096075	16.099670162381596	19.163354194857916	26.463654431664413
14	24.76372209066306	19.650181833558864	22.106520635994585	33.479575439783495
15	31.883245940460082	21.98600304465494	18.660668978349122	27.47008203653586
16	25.67817574424899	22.60444857916103	22.149864682002708	29.56751099458728
17	27.945280784844385	22.135592861975642	20.692553281461436	29.226573071718537
18	27.3886798037889	19.405975135317995	22.186865696887686	31.018479364005415
19	29.590768775372123	21.732281799729364	21.369671853856563	27.30727757104195
20	31.454562753721245	18.609924729364007	21.18889546684709	28.74661705006766
21	29.204372462787553	20.09419401217862	20.85694350473613	29.844490020297698
22	28.998752537212447	21.623921684709067	19.66551082543978	29.711814952638697
23	30.354575439783492	20.019663396481732	20.811485115020297	28.814276048714476
24	29.321189952638697	19.354173714479025	21.406144282814616	29.91849205006766
25	30.606710926928283	20.93411705006766	21.31469891745602	27.144473105548037
26	30.35510402571042	21.44737398511502	20.93041694857916	27.267105040595403
27	28.551568843031124	20.536091847090663	23.70232154939107	27.210017760487144
28	27.56258457374831	22.942743572395127	20.426674560216508	29.06799729364005
29	28.515625	23.26200947225981	19.975790764546687	28.246574763193504
30	28.471223782138022	21.13022242895805	23.289495940460082	27.10905784844384
31	29.618255243572396	20.805142083897156	21.1217650541272	28.45483761840325
32	26.536599289580515	20.62700862652233	22.141935893098783	30.694456190798373
33	27.295648680649524	20.471075778078486	23.355569181326118	28.877706359945872
34	26.68883203653586	20.82892845060893	23.04793217185386	29.434307341001354
35	26.78979194857916	22.822754566982407	23.46128636671177	26.926167117726656
36	27.546198410013535	20.763912381596754	24.163777063599458	27.526112144790254
37	28.048355040595403	20.16819604194858	23.52894536535859	28.254503552097425
38	26.252748646820027	19.363688261163734	25.306051251691475	29.077511840324767
39	25.708305142083898	18.203442151556157	27.0054550067659	29.08279769959405
40	25.538629059539918	19.53125	27.96166694857916	26.96845399188092
41	23.60717608254398	18.687098274695536	28.667857746955345	29.037867895805142
42	21.759239682002708	19.585694350473613	30.649526387009473	28.00553958051421
43	20.90610199594046	20.63493741542625	28.799475642760484	29.659484945872798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	6.5
7	11.0
8	12.5
9	14.0
10	19.5
11	25.0
12	25.0
13	52.5
14	80.0
15	135.0
16	190.0
17	180.0
18	170.0
19	170.0
20	202.5
21	235.0
22	160.5
23	86.0
24	80.5
25	75.0
26	75.0
27	72.0
28	69.0
29	72.0
30	75.0
31	81.5
32	88.0
33	88.0
34	345.5
35	603.0
36	510.5
37	418.0
38	803.0
39	1188.0
40	1188.0
41	1964.5
42	2741.0
43	4090.5
44	5440.0
45	9157.5
46	12875.0
47	12875.0
48	15383.0
49	17891.0
50	19610.5
51	21330.0
52	22098.5
53	22867.0
54	22867.0
55	21999.5
56	21132.0
57	20145.0
58	19158.0
59	17931.0
60	16704.0
61	16704.0
62	15687.0
63	14670.0
64	11972.5
65	9275.0
66	8353.5
67	7432.0
68	7432.0
69	6569.0
70	5706.0
71	4774.5
72	3843.0
73	3055.5
74	2268.0
75	2268.0
76	1772.5
77	1277.0
78	979.5
79	682.0
80	517.5
81	353.0
82	353.0
83	246.5
84	140.0
85	93.0
86	46.0
87	32.0
88	18.0
89	18.0
90	11.5
91	5.0
92	2.5
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	189184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.10451200947226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20689222271044	35.454901048714476
2	7.011487037850732	5.904304803788904
3	2.330048333437951	2.943166441136671
4	1.301864289749545	2.1925744248985115
5	0.8511706735295964	1.7919062922868743
6	0.563680873768125	1.4240104871447903
7	0.44692737430167595	1.3172361299052775
8	0.32640763291695435	1.0994587280108254
9	0.2824681438704413	1.0703865020297698
>10	2.148013307388111	18.133140223274697
>50	0.28874521373422884	8.486975642760488
>100	0.2360178268784132	18.37734692151556
>500	0.006277069863787584	1.8045923545331528
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	946	0.5000422868741542	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	700	0.37001014884979705	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	670	0.3541525710419486	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	577	0.3049940798376184	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	521	0.27539326792963464	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	480	0.25372124492557513	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	476	0.251606901217862	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	409	0.21619164411366712	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	396	0.20932002706359945	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	393	0.20773426928281463	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	391	0.20667709742895804	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	386	0.20403416779431666	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	384	0.20297699594046006	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	384	0.20297699594046006	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	380	0.20086265223274694	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	376	0.19874830852503383	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	370	0.19557679296346417	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	358	0.18923376184032475	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	355	0.18764800405953994	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	355	0.18764800405953994	Illumina PCR Primer Index 10 (95% over 21bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	328	0.17337618403247632	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	325	0.17179042625169147	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	321	0.16967608254397834	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	310	0.16386163734776724	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	302	0.159632949932341	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	301	0.15910436400541272	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	299	0.15804719215155616	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	296	0.1564614343707713	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	296	0.1564614343707713	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	291	0.1538185047361299	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	282	0.14906123139377536	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	279	0.14747547361299052	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	275	0.1453611299052774	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	274	0.1448325439783491	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	273	0.14430395805142085	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	262	0.13848951285520975	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	260	0.13743234100135318	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	257	0.13584658322056833	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	243	0.1284463802435724	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	242	0.1279177943166441	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	241	0.12738920838971582	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	234	0.12368910690121786	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	233	0.12316052097428958	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	233	0.12316052097428958	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	232	0.1226319350473613	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	229	0.12104617726657646	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	228	0.12051759133964816	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	227	0.1199890054127199	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	226	0.11946041948579161	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	224	0.11840324763193505	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	223	0.11787466170500675	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	222	0.11734607577807848	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	222	0.11734607577807848	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	221	0.1168174898511502	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	217	0.11470314614343707	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	213	0.11258880243572396	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	212	0.11206021650879566	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	210	0.11100304465493911	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	206	0.10888870094722597	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	206	0.10888870094722597	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	205	0.1083601150202977	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	205	0.1083601150202977	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	203	0.10730294316644114	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	202	0.10677435723951285	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	199	0.10518859945872802	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	197	0.10413142760487144	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	196	0.10360284167794317	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	194	0.10254566982408661	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	193	0.10201708389715833	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	192	0.10148849797023003	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	190	0.10043132611637347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.285859269282815E-4
13	0.0	0.0	0.0	0.0	5.285859269282815E-4
14	0.0	0.0	0.0	0.0	5.285859269282815E-4
15	0.0	0.0	0.0	0.0	5.285859269282815E-4
16	0.0	0.0	5.285859269282815E-4	0.0	5.285859269282815E-4
17	0.0	0.0	5.285859269282815E-4	0.0	0.001057171853856563
18	5.285859269282815E-4	0.0	5.285859269282815E-4	0.0	0.001057171853856563
19	5.285859269282815E-4	0.0	5.285859269282815E-4	0.0	0.001057171853856563
20	5.285859269282815E-4	0.0	5.285859269282815E-4	0.0	0.001057171853856563
21	5.285859269282815E-4	0.0	5.285859269282815E-4	0.001057171853856563	0.001057171853856563
22	5.285859269282815E-4	0.0	5.285859269282815E-4	0.003171515561569689	0.001057171853856563
23	5.285859269282815E-4	0.0	5.285859269282815E-4	0.0037001014884979703	0.001057171853856563
24	5.285859269282815E-4	0.0	5.285859269282815E-4	0.006343031123139378	0.001057171853856563
25	5.285859269282815E-4	0.0	5.285859269282815E-4	0.007400202976995941	0.001057171853856563
26	5.285859269282815E-4	0.0	5.285859269282815E-4	0.016914749661705007	0.001057171853856563
27	5.285859269282815E-4	0.0	5.285859269282815E-4	0.07083051420838972	0.001057171853856563
28	5.285859269282815E-4	0.0	5.285859269282815E-4	0.23892083897158323	0.001057171853856563
29	5.285859269282815E-4	0.0	5.285859269282815E-4	0.46568420162381596	0.001057171853856563
30	5.285859269282815E-4	0.0	5.285859269282815E-4	0.7579922192151556	0.001057171853856563
31	5.285859269282815E-4	0.0	5.285859269282815E-4	1.3722090663058186	0.001057171853856563
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAT	20	0.0018380514	37.0	1
TCAATGC	20	0.0018380514	37.0	32
CAATGCT	20	0.0018380514	37.0	33
ATACGGC	30	8.275294E-6	37.0	29
TACGGCT	35	2.3778504E-5	31.714285	30
CGTTATG	25	0.005484885	29.6	2
AGACCCG	25	0.005484885	29.6	5
TGCAGGT	25	0.005484885	29.6	16
GGTATCA	305	0.0	28.508196	1
AAGTCTG	60	1.3275621E-6	24.666668	36
TAACCCT	45	1.3175112E-4	24.666666	37
CATGGGA	40	0.0019252602	23.125002	26
ATGGGAG	40	0.0019252602	23.125002	27
TGGGAGT	40	0.0019252602	23.125002	28
CTAAGTC	65	2.6632224E-6	22.76923	34
TAAGTCT	65	2.6632224E-6	22.76923	35
GGTAATA	345	0.0	22.521738	25
TAATACG	330	0.0	22.424242	27
TATGCCG	50	2.6905545E-4	22.2	33
GCGGTAA	365	0.0	21.28767	23
>>END_MODULE