FastQCFastQC Report
Fri 10 Feb 2017
ERR1632458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891991
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA24410.2736574696381466No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17470.19585399404254078No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14760.16547252158373796No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13000.1457413807986852No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12400.13901485553105358No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11310.12679500129485613No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10920.12242275987089556No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10540.11816262720139553No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10500.11771419218355342No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10360.11614466962110603No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10130.11356616826851391No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9930.11132399317930339No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9740.10919392684455337No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9650.10818494805440862No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9110.10213107531354015No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT9060.10157053154123752No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC8960.10044944399663226No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT8920.10000100897879015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT200.001841714737.034
GGTATCA10900.029.5321121
GTTTATA400.001930849523.1250021
CTATACC652.681727E-622.769234
ATACGGC1550.022.67741829
CTACTAA502.7014452E-422.1999991
CGTATGC806.956525E-720.812531
CAGTCGG1800.020.55555510
ACTATAC555.141853E-420.1818183
GGTAATA10150.020.04926125
CTTATAC10250.020.03414537
TAATACG9400.019.87765927
GTATCAA16300.019.6349722
ACTCTAA851.244669E-619.58823410
GAACACT1052.2577296E-819.3809536
GCGGTAA10550.019.11374323
GCAGTCG1850.019.09
GTAATAC10700.018.67289726
CTCGTAT902.1511169E-618.529
CCCTATA701.2188086E-418.52
Produced by FastQC (version 0.11.5)