##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632458.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891991
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06200510991703	34.0	33.0	34.0	31.0	34.0
2	33.19847621780937	34.0	33.0	34.0	31.0	34.0
3	33.26816189849449	34.0	34.0	34.0	31.0	34.0
4	36.55032954368374	37.0	37.0	37.0	35.0	37.0
5	36.52333823995982	37.0	37.0	37.0	35.0	37.0
6	36.56702702157309	37.0	37.0	37.0	35.0	37.0
7	36.56571871240853	37.0	37.0	37.0	35.0	37.0
8	36.54085635393182	37.0	37.0	37.0	35.0	37.0
9	38.33266367037336	39.0	39.0	39.0	37.0	39.0
10	38.365021620173295	39.0	39.0	39.0	37.0	39.0
11	38.43068035439819	39.0	39.0	39.0	37.0	39.0
12	38.39569681756879	39.0	39.0	39.0	37.0	39.0
13	38.42492132768156	39.0	39.0	39.0	37.0	39.0
14	39.974118572945244	41.0	40.0	41.0	38.0	41.0
15	39.97227550502191	41.0	40.0	41.0	38.0	41.0
16	39.93579419523291	41.0	40.0	41.0	38.0	41.0
17	39.942405248483446	41.0	40.0	41.0	38.0	41.0
18	39.936743756383194	41.0	40.0	41.0	38.0	41.0
19	39.94145232407053	41.0	40.0	41.0	38.0	41.0
20	39.9140282805544	41.0	40.0	41.0	38.0	41.0
21	39.87346172775286	41.0	40.0	41.0	38.0	41.0
22	39.8506767444963	41.0	40.0	41.0	38.0	41.0
23	39.813447669315046	41.0	40.0	41.0	38.0	41.0
24	39.785705236936245	41.0	40.0	41.0	38.0	41.0
25	39.75165108168132	41.0	40.0	41.0	38.0	41.0
26	39.66516590414029	41.0	40.0	41.0	37.0	41.0
27	39.574152654006596	41.0	40.0	41.0	37.0	41.0
28	39.53307824854735	41.0	40.0	41.0	37.0	41.0
29	39.49356551803774	41.0	40.0	41.0	37.0	41.0
30	39.45421646630964	41.0	39.0	41.0	37.0	41.0
31	39.395991663593016	41.0	39.0	41.0	36.0	41.0
32	39.34058751713862	41.0	39.0	41.0	36.0	41.0
33	39.274674296041105	41.0	39.0	41.0	36.0	41.0
34	39.234391378388345	41.0	39.0	41.0	35.0	41.0
35	39.153850207008816	41.0	39.0	41.0	35.0	41.0
36	39.094072698042915	41.0	39.0	41.0	35.0	41.0
37	39.033634868513246	41.0	39.0	41.0	35.0	41.0
38	38.95793118988869	41.0	39.0	41.0	35.0	41.0
39	38.86540447156978	40.0	39.0	41.0	35.0	41.0
40	38.78682296121822	40.0	38.0	41.0	35.0	41.0
41	38.73159370442078	40.0	38.0	41.0	35.0	41.0
42	38.65995733140805	40.0	38.0	41.0	35.0	41.0
43	37.93984020018139	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	3.0
17	5.0
18	18.0
19	33.0
20	66.0
21	92.0
22	216.0
23	362.0
24	578.0
25	780.0
26	1114.0
27	1464.0
28	2043.0
29	2774.0
30	3774.0
31	4948.0
32	6639.0
33	8880.0
34	13813.0
35	21436.0
36	34438.0
37	65515.0
38	171188.0
39	551810.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.69157424234101	16.755998659179298	12.580283881788045	27.97214321669165
2	21.65089109643483	19.592462255785094	31.94796808487978	26.8086785629003
3	21.42577671747809	19.71510923316491	28.851524286680025	30.00758976267698
4	15.983457232191805	15.758903397007368	33.59148242527111	34.66615694552972
5	17.829888418156685	32.42073070243982	32.15110914796225	17.598271731441237
6	34.243731158722454	33.3097531253118	15.302508657598562	17.14400705836718
7	30.04413721663111	27.560928305330435	19.716902973236277	22.678031504802178
8	26.878410208174746	30.605465750214968	20.274307700414017	22.24181634119627
9	27.987950551070583	13.163137296228324	17.66878813799691	41.180124014704184
10	19.397168805514852	23.239135820877117	29.524961574724408	27.83873379888362
11	39.3953526436926	19.457371206660156	18.76678127918331	22.38049487046394
12	23.22276794272588	23.839253983504317	26.2645026687489	26.673475405020902
13	34.89822206726301	17.447597565446287	20.979471765970732	26.674708601319967
14	24.190939146246993	20.235518071370677	22.660206212842954	32.91333656953938
15	29.611397424413475	24.439372146131518	19.463649296909946	26.48558113254506
16	25.89218949518549	23.874231914896	22.13374350189632	28.09983508802219
17	27.034801920647183	23.498107043680935	21.7165868265487	27.750504209123182
18	26.703296333707407	21.223084089413458	23.56940821151783	28.50421136536131
19	28.477641590554164	22.293946912020413	23.076914453172733	26.151497044252686
20	29.59559009003454	21.072522032172973	22.69137244658298	26.64051543120951
21	27.74658040271707	22.158967971649936	21.82533231837541	28.269119307257583
22	27.91687360074261	22.98453683949726	21.166917603428733	27.9316719563314
23	27.892882327288053	21.84764196051305	22.33396973736282	27.925505974836067
24	27.94703085569249	21.84248495780787	22.37612262903998	27.83436155745966
25	28.18156237002391	22.241031579915045	22.62007127874609	26.957334771314954
26	28.38447921559747	22.64596840102647	21.97398852679007	26.995563856585996
27	27.27942322288005	21.907171709131596	23.546874351871264	27.266530716117092
28	26.494998267919744	23.046869306977314	21.970401046647332	28.48773137845561
29	26.89130271493771	23.545865373081117	22.30201874234157	27.260813169639604
30	26.677287102672558	22.565025880305967	23.762459486698855	26.995227530322612
31	28.322258856871873	22.25325143415124	22.175896393573478	27.248593315403408
32	25.140612405282113	22.243385863758718	22.809198747520995	29.806802983438175
33	26.105644563678332	21.77656501018508	24.22401122881285	27.89377919732374
34	26.343987775661414	21.780937251609043	23.766943836877278	28.108131135852265
35	26.499146291834784	23.1079685781583	23.965712658535793	26.42717247147112
36	26.382665295950297	21.863000859874145	24.57962019796164	27.17471364621392
37	26.71136816402856	21.24898121169384	24.35719642911195	27.682454195165647
38	25.95698835526367	20.724648567081953	25.787704136028278	27.53065894162609
39	25.055297643137653	19.830917576522634	26.906213179280957	28.207571601058756
40	25.047562139079876	20.971175718140653	27.40532135413922	26.575940788640246
41	23.655395626189055	19.983609700097873	28.614302162241543	27.746692511471526
42	22.408746276587994	20.900435094076062	29.175406478316486	27.515412151019458
43	21.443602009437317	21.374991451707473	28.881233106612065	28.300173432243152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	10.5
2	10.0
3	21.5
4	33.0
5	33.0
6	42.5
7	52.0
8	56.0
9	60.0
10	98.5
11	137.0
12	137.0
13	266.0
14	395.0
15	657.5
16	920.0
17	930.5
18	941.0
19	941.0
20	1151.5
21	1362.0
22	1313.5
23	1265.0
24	1558.5
25	1852.0
26	1852.0
27	2242.0
28	2632.0
29	3635.0
30	4638.0
31	5626.5
32	6615.0
33	6615.0
34	8335.5
35	10056.0
36	11698.0
37	13340.0
38	18135.0
39	22930.0
40	22930.0
41	26135.5
42	29341.0
43	33969.0
44	38597.0
45	49498.0
46	60399.0
47	60399.0
48	68236.0
49	76073.0
50	86097.5
51	96122.0
52	100095.5
53	104069.0
54	104069.0
55	97095.5
56	90122.0
57	86427.0
58	82732.0
59	76689.0
60	70646.0
61	70646.0
62	63704.5
63	56763.0
64	46844.5
65	36926.0
66	31914.5
67	26903.0
68	26903.0
69	23015.5
70	19128.0
71	16780.5
72	14433.0
73	11449.5
74	8466.0
75	8466.0
76	6833.0
77	5200.0
78	4254.5
79	3309.0
80	2731.5
81	2154.0
82	2154.0
83	1812.5
84	1471.0
85	1232.0
86	993.0
87	747.5
88	502.0
89	502.0
90	401.0
91	300.0
92	178.5
93	57.0
94	41.5
95	26.0
96	26.0
97	18.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	891991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.25925564798769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41449028146111	35.67293525198838
2	7.375077985854142	6.233306120561126
3	2.2969526115650685	2.9120252287032358
4	1.201048341753298	2.030216356789772
5	0.7865387856124314	1.6619271809126757
6	0.5620997272191253	1.4252349643330295
7	0.3965080632078598	1.1729294926712586
8	0.2956576770140098	0.9995418685780171
9	0.247870841086126	0.9427353517026296
>10	1.9223077240060533	16.551882420547255
>50	0.2908452618040126	8.541331804678872
>100	0.19274145782070215	15.835762215296235
>500	0.014928798945966661	4.377630779571197
>1k	0.002932442650100594	1.6425409636663761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2441	0.2736574696381466	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1747	0.19585399404254078	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1476	0.16547252158373796	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1300	0.1457413807986852	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1240	0.13901485553105358	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1131	0.12679500129485613	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1092	0.12242275987089556	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1054	0.11816262720139553	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1050	0.11771419218355342	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1036	0.11614466962110603	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1013	0.11356616826851391	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	993	0.11132399317930339	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	974	0.10919392684455337	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	965	0.10818494805440862	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	911	0.10213107531354015	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	906	0.10157053154123752	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	896	0.10044944399663226	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	892	0.10000100897879015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1210875446052707E-4	0.0
9	0.0	0.0	0.0	1.1210875446052707E-4	0.0
10	0.0	0.0	0.0	1.1210875446052707E-4	0.0
11	0.0	0.0	0.0	1.1210875446052707E-4	0.0
12	1.1210875446052707E-4	0.0	0.0	1.1210875446052707E-4	0.0
13	1.1210875446052707E-4	0.0	0.0	1.1210875446052707E-4	0.0
14	1.1210875446052707E-4	0.0	0.0	1.1210875446052707E-4	0.0
15	1.1210875446052707E-4	0.0	0.0	1.1210875446052707E-4	0.0
16	1.1210875446052707E-4	0.0	0.0	7.847612812236894E-4	0.0
17	1.1210875446052707E-4	0.0	0.0	0.0012331962990657977	0.0
18	2.2421750892105414E-4	0.0	0.0	0.0013453050535263248	0.0
19	2.2421750892105414E-4	0.0	0.0	0.0017937400713684331	0.0
20	2.2421750892105414E-4	0.0	0.0	0.0025785013525921226	0.0
21	2.2421750892105414E-4	0.0	0.0	0.004484350178421082	0.0
22	2.2421750892105414E-4	0.0	0.0	0.006726525267631624	0.0
23	2.2421750892105414E-4	0.0	0.0	0.008520265339000058	0.0
24	2.2421750892105414E-4	0.0	0.0	0.011322984200513234	0.0
25	2.2421750892105414E-4	0.0	0.0	0.013453050535263248	0.0
26	2.2421750892105414E-4	0.0	1.1210875446052707E-4	0.018049509468144858	0.0
27	2.2421750892105414E-4	0.0	1.1210875446052707E-4	0.04170445665931607	0.0
28	2.2421750892105414E-4	0.0	1.1210875446052707E-4	0.14394764072731675	0.0
29	2.2421750892105414E-4	0.0	1.1210875446052707E-4	0.289913239034923	0.0
30	3.363262633815812E-4	0.0	1.1210875446052707E-4	0.4863277768497664	0.0
31	3.363262633815812E-4	0.0	1.1210875446052707E-4	1.0030370261583357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTCT	20	0.0018417147	37.0	34
GGTATCA	1090	0.0	29.532112	1
GTTTATA	40	0.0019308495	23.125002	1
CTATACC	65	2.681727E-6	22.76923	4
ATACGGC	155	0.0	22.677418	29
CTACTAA	50	2.7014452E-4	22.199999	1
CGTATGC	80	6.956525E-7	20.8125	31
CAGTCGG	180	0.0	20.555555	10
ACTATAC	55	5.141853E-4	20.181818	3
GGTAATA	1015	0.0	20.049261	25
CTTATAC	1025	0.0	20.034145	37
TAATACG	940	0.0	19.877659	27
GTATCAA	1630	0.0	19.634972	2
ACTCTAA	85	1.244669E-6	19.588234	10
GAACACT	105	2.2577296E-8	19.380953	6
GCGGTAA	1055	0.0	19.113743	23
GCAGTCG	185	0.0	19.0	9
GTAATAC	1070	0.0	18.672897	26
CTCGTAT	90	2.1511169E-6	18.5	29
CCCTATA	70	1.2188086E-4	18.5	2
>>END_MODULE