FastQCFastQC Report
Fri 10 Feb 2017
ERR1632457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358437
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT52640.2231986692881769No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT51860.21989139417334444No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA41010.17388634930676547No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA33150.14055919238037734No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC26490.11232015101526986No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA26090.11062411249484298No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG26020.11032730575376828No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA25750.10918247975248013No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG25010.10604480848969043No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT24860.10540879404453034No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23800.10091429196539911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.028.590911
GACAGGC15900.021.2924527
ATACACA5700.020.44736937
ATACGGC3150.019.96825429
CTTATAC29500.019.62881537
CATCAGA17650.019.60056716
GTATCAA35550.019.4106882
ACAGGCT17850.019.0700268
CCAGGAC19950.018.9172943
TAAGGTA2350.018.8936185
GCGGTAA18100.018.7044223
GTACTAA609.2407427E-418.51
AGAAGAG18400.018.39945620
TTCTGCG11850.018.26582318
ATCAGAA18650.018.25201217
GGACAGG19250.017.8753266
TTAGCTA2800.017.8392854
AGGCCAT18850.017.76392625
TCCAGGA21650.017.6882212
TGCGGGT12350.017.67611321
Produced by FastQC (version 0.11.5)