##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2358437
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04376839406777	34.0	33.0	34.0	31.0	34.0
2	33.188158937465786	34.0	33.0	34.0	31.0	34.0
3	33.25891851255726	34.0	34.0	34.0	31.0	34.0
4	36.53965401662202	37.0	37.0	37.0	35.0	37.0
5	36.511728318373564	37.0	37.0	37.0	35.0	37.0
6	36.55089960003171	37.0	37.0	37.0	35.0	37.0
7	36.549947698412126	37.0	37.0	37.0	35.0	37.0
8	36.51833990053582	37.0	37.0	37.0	35.0	37.0
9	38.32092356081591	39.0	39.0	39.0	37.0	39.0
10	38.35277601224879	39.0	39.0	39.0	37.0	39.0
11	38.423314678322974	39.0	39.0	39.0	37.0	39.0
12	38.3919481419262	39.0	39.0	39.0	37.0	39.0
13	38.41377700570335	39.0	39.0	39.0	37.0	39.0
14	39.98216233887104	41.0	40.0	41.0	38.0	41.0
15	39.96940600914928	41.0	40.0	41.0	38.0	41.0
16	39.94560634861139	41.0	40.0	41.0	38.0	41.0
17	39.93655543904713	41.0	40.0	41.0	38.0	41.0
18	39.901123074307264	41.0	40.0	41.0	38.0	41.0
19	39.910679403350606	41.0	40.0	41.0	38.0	41.0
20	39.86780482158311	41.0	40.0	41.0	38.0	41.0
21	39.849319697748975	41.0	40.0	41.0	38.0	41.0
22	39.82106920812385	41.0	40.0	41.0	38.0	41.0
23	39.78182796487674	41.0	40.0	41.0	38.0	41.0
24	39.7621216933079	41.0	40.0	41.0	38.0	41.0
25	39.70721244620908	41.0	40.0	41.0	37.0	41.0
26	39.62223031609494	41.0	40.0	41.0	37.0	41.0
27	39.55601951631525	41.0	40.0	41.0	37.0	41.0
28	39.497003311939224	41.0	40.0	41.0	37.0	41.0
29	39.45755430397335	41.0	40.0	41.0	37.0	41.0
30	39.406536617259654	41.0	39.0	41.0	36.0	41.0
31	39.36409028521856	41.0	39.0	41.0	36.0	41.0
32	39.31642354661159	41.0	39.0	41.0	36.0	41.0
33	39.24330944604414	41.0	39.0	41.0	35.0	41.0
34	39.18964721126746	41.0	39.0	41.0	35.0	41.0
35	39.10252001643461	41.0	39.0	41.0	35.0	41.0
36	39.06609462114103	41.0	39.0	41.0	35.0	41.0
37	39.0129526461805	41.0	39.0	41.0	35.0	41.0
38	38.9393844312992	41.0	39.0	41.0	35.0	41.0
39	38.85756583703529	40.0	39.0	41.0	35.0	41.0
40	38.74873274121802	40.0	38.0	41.0	35.0	41.0
41	38.69622042055819	40.0	38.0	41.0	35.0	41.0
42	38.627532980529054	40.0	38.0	41.0	35.0	41.0
43	37.88174329015361	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	5.0
16	10.0
17	14.0
18	31.0
19	75.0
20	105.0
21	249.0
22	489.0
23	851.0
24	1358.0
25	2097.0
26	2837.0
27	4083.0
28	5723.0
29	7907.0
30	10287.0
31	13708.0
32	17865.0
33	23942.0
34	36422.0
35	63480.0
36	95363.0
37	173349.0
38	450726.0
39	1447457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01758452737979	15.380101312861017	11.721364615633151	28.880949544126043
2	21.60430827704959	17.81302616945036	32.362535017895325	28.220130535604724
3	21.924096340076076	18.953824079252488	27.72526889630717	31.39681068436426
4	16.253306745102794	14.578214300403191	31.99538507918592	37.1730938753081
5	18.13985279233662	32.22481669003666	31.924957079625194	17.710373438001522
6	37.11084926160843	31.573707502044783	14.4658093474619	16.849633888884885
7	32.1276336828162	26.410796642013334	18.896455576299047	22.565114098871415
8	28.60292642966507	30.37617710373438	18.83654301556497	22.18435345103558
9	28.65317157083272	12.777445401339953	16.65425025133171	41.91513277649562
10	20.24391577981519	22.650424836448884	28.886631273169478	28.219028110566448
11	39.699343251483924	19.011701393762053	18.366825147332747	22.922130207421272
12	24.635892330386607	22.364727147682977	25.553915580530667	27.44546494139975
13	34.30496553437722	17.008001485729743	20.58274187523347	28.104291104659566
14	24.804012148723924	18.72557969536604	22.23922029717139	34.23118785873865
15	30.18363433070292	22.888167036049722	19.633935525943667	27.294263107303696
16	27.072972481350998	22.864422496763748	20.79029458917071	29.272310432714548
17	27.540739905284727	22.804933945659776	21.479607044835202	28.174719104220298
18	26.87436637060901	20.766041238328604	23.15287624812535	29.20671614293704
19	29.05445428476572	21.328108403998073	22.032091592864255	27.585345718371958
20	28.77889042615936	20.860849791620467	22.503293494801856	27.856966287418324
21	29.270741597083155	21.15583329128571	21.274810393493656	28.298614718137475
22	29.536044422640927	22.216238975219603	20.438366596182135	27.809350005957334
23	29.06679296500182	21.978115166951675	21.197174230221115	27.75791763782539
24	29.318527482396178	21.13620164541177	21.041053884415824	28.504216987776225
25	28.682301032421048	21.307035125381766	22.178332514288066	27.83233132790912
26	29.08197250975964	21.607954759868505	21.958059511447626	27.352013218924228
27	29.064672916851286	21.000942573407727	22.346961144181506	27.587423365559477
28	27.226421566486618	21.712685138504867	22.336064096687764	28.72482919832075
29	28.60564009129775	21.862530141784582	22.025731448412657	27.50609831850501
30	27.615111194405443	21.042410715232165	23.171193464145958	28.17128462621643
31	27.76415057938796	21.40621097786373	22.178968528733225	28.650669914015086
32	26.190099629542786	21.517089496136634	22.780383788076595	29.51242708624398
33	27.505971115615978	21.358679498328765	23.46091924439788	27.674430141657375
34	27.36206224715776	21.367371695745955	23.329857867731892	27.9407081893644
35	27.552060962408575	21.960094757672135	23.440778786967808	27.04706549295148
36	27.29405110248864	20.759299485209908	23.431323372216433	28.51532604008502
37	26.32985320362596	20.650032203531406	24.168760920898038	28.851353671944597
38	26.655195792806847	19.76058720245654	25.45936143301687	28.124855571719742
39	25.376594753220033	19.210265103541033	26.59744568118631	28.815694462052623
40	25.437864144770455	19.68358705362916	27.82211269582355	27.056436105776832
41	23.785625819133603	19.17418188401895	28.405931555517487	28.63426074132996
42	22.196056116826526	20.188243315382177	29.16219513177583	28.453505436015465
43	21.948392091881193	19.755753492673325	29.25797890721694	29.037875508228545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	16.5
2	11.0
3	44.0
4	77.0
5	77.0
6	123.5
7	170.0
8	162.0
9	154.0
10	221.0
11	288.0
12	288.0
13	619.0
14	950.0
15	1568.5
16	2187.0
17	2085.0
18	1983.0
19	1983.0
20	2426.5
21	2870.0
22	2373.0
23	1876.0
24	2215.5
25	2555.0
26	2555.0
27	3306.5
28	4058.0
29	5745.0
30	7432.0
31	10188.0
32	12944.0
33	12944.0
34	18558.5
35	24173.0
36	28624.5
37	33076.0
38	44218.0
39	55360.0
40	55360.0
41	64207.5
42	73055.0
43	86359.5
44	99664.0
45	118649.0
46	137634.0
47	137634.0
48	156158.0
49	174682.0
50	197982.0
51	221282.0
52	239894.0
53	258506.0
54	258506.0
55	249451.0
56	240396.0
57	226454.5
58	212513.0
59	199653.0
60	186793.0
61	186793.0
62	181117.5
63	175442.0
64	143696.5
65	111951.0
66	97018.5
67	82086.0
68	82086.0
69	71304.0
70	60522.0
71	54664.0
72	48806.0
73	49564.0
74	50322.0
75	50322.0
76	49016.5
77	47711.0
78	31713.0
79	15715.0
80	10476.5
81	5238.0
82	5238.0
83	4070.5
84	2903.0
85	2264.0
86	1625.0
87	1199.5
88	774.0
89	774.0
90	580.5
91	387.0
92	275.5
93	164.0
94	112.5
95	61.0
96	61.0
97	39.0
98	17.0
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2358437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.78404031194794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.53925536819796	29.535880414137605
2	8.8450945664292	6.330264410561884
3	2.648297380963442	2.843003407152658
4	1.3022473844840028	1.863986916100173
5	0.7938908465118825	1.4204311027433838
6	0.5472649285283818	1.1750010158264954
7	0.3977244257403663	0.9962530818617729
8	0.3012442043323087	0.8623787801254412
9	0.23853015335672267	0.7682015360998878
>10	1.8329570275060194	13.15826204346979
>50	0.2643167908644109	6.685987189282986
>100	0.23848269820847698	17.428291614368174
>500	0.03629516100344537	8.912961186863718
>1k	0.014161062817737699	7.574102894134942
>5k	2.380010557602975E-4	0.4449944072712612
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	5264	0.2231986692881769	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	5186	0.21989139417334444	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	4101	0.17388634930676547	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3315	0.14055919238037734	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	2649	0.11232015101526986	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2609	0.11062411249484298	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2602	0.11032730575376828	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2575	0.10918247975248013	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2501	0.10604480848969043	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2486	0.10540879404453034	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2380	0.10091429196539911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.24009630106719E-5	0.0	0.0	4.24009630106719E-5	0.0
3	4.24009630106719E-5	0.0	0.0	4.24009630106719E-5	0.0
4	4.24009630106719E-5	0.0	0.0	4.24009630106719E-5	0.0
5	8.48019260213438E-5	4.24009630106719E-5	0.0	4.24009630106719E-5	0.0
6	8.48019260213438E-5	4.24009630106719E-5	0.0	4.24009630106719E-5	0.0
7	8.48019260213438E-5	4.24009630106719E-5	0.0	4.24009630106719E-5	0.0
8	1.2720288903201568E-4	4.24009630106719E-5	0.0	4.24009630106719E-5	4.24009630106719E-5
9	1.2720288903201568E-4	4.24009630106719E-5	0.0	8.48019260213438E-5	4.24009630106719E-5
10	1.696038520426876E-4	4.24009630106719E-5	0.0	8.48019260213438E-5	4.24009630106719E-5
11	2.1200481505335948E-4	4.24009630106719E-5	0.0	1.2720288903201568E-4	4.24009630106719E-5
12	2.5440577806403136E-4	4.24009630106719E-5	0.0	1.2720288903201568E-4	8.48019260213438E-5
13	2.5440577806403136E-4	4.24009630106719E-5	0.0	1.2720288903201568E-4	8.48019260213438E-5
14	2.5440577806403136E-4	4.24009630106719E-5	0.0	2.5440577806403136E-4	8.48019260213438E-5
15	2.5440577806403136E-4	4.24009630106719E-5	0.0	4.2400963010671896E-4	8.48019260213438E-5
16	2.968067410747033E-4	4.24009630106719E-5	0.0	8.056182972027661E-4	8.48019260213438E-5
17	3.392077040853752E-4	4.24009630106719E-5	0.0	0.0011448260012881413	8.48019260213438E-5
18	3.392077040853752E-4	4.24009630106719E-5	0.0	0.0012296279273094851	8.48019260213438E-5
19	3.392077040853752E-4	4.24009630106719E-5	0.0	0.0017384394834375478	8.48019260213438E-5
20	3.392077040853752E-4	4.24009630106719E-5	0.0	0.0023320529655869544	8.48019260213438E-5
21	3.392077040853752E-4	4.24009630106719E-5	0.0	0.0041128934120351745	8.48019260213438E-5
22	3.816086670960471E-4	4.24009630106719E-5	0.0	0.006275342525579441	8.48019260213438E-5
23	3.816086670960471E-4	4.24009630106719E-5	0.0	0.007504970452888926	8.48019260213438E-5
24	3.816086670960471E-4	4.24009630106719E-5	0.0	0.009243409936326474	1.2720288903201568E-4
25	3.816086670960471E-4	4.24009630106719E-5	0.0	0.01093944845675335	1.2720288903201568E-4
26	3.816086670960471E-4	4.24009630106719E-5	0.0	0.016027564018033978	1.2720288903201568E-4
27	3.816086670960471E-4	4.24009630106719E-5	0.0	0.04507222368034423	1.2720288903201568E-4
28	4.2400963010671896E-4	4.24009630106719E-5	0.0	0.17494637338203226	1.696038520426876E-4
29	4.2400963010671896E-4	4.24009630106719E-5	0.0	0.3515463843214807	1.696038520426876E-4
30	4.6641059311739087E-4	4.24009630106719E-5	0.0	0.58877977236619	1.696038520426876E-4
31	4.6641059311739087E-4	4.24009630106719E-5	0.0	1.2451042788083804	1.696038520426876E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2420	0.0	28.59091	1
GACAGGC	1590	0.0	21.292452	7
ATACACA	570	0.0	20.447369	37
ATACGGC	315	0.0	19.968254	29
CTTATAC	2950	0.0	19.628815	37
CATCAGA	1765	0.0	19.600567	16
GTATCAA	3555	0.0	19.410688	2
ACAGGCT	1785	0.0	19.070026	8
CCAGGAC	1995	0.0	18.917294	3
TAAGGTA	235	0.0	18.893618	5
GCGGTAA	1810	0.0	18.70442	23
GTACTAA	60	9.2407427E-4	18.5	1
AGAAGAG	1840	0.0	18.399456	20
TTCTGCG	1185	0.0	18.265823	18
ATCAGAA	1865	0.0	18.252012	17
GGACAGG	1925	0.0	17.875326	6
TTAGCTA	280	0.0	17.839285	4
AGGCCAT	1885	0.0	17.763926	25
TCCAGGA	2165	0.0	17.688221	2
TGCGGGT	1235	0.0	17.676113	21
>>END_MODULE