FastQCFastQC Report
Fri 10 Feb 2017
ERR1632456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718904
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15800.21977899691753003No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12370.17206748049809154No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10820.1505068826992199No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10750.14953317828249668No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9620.13381480698396447No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8430.1172618318996695No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8370.11642722811390671No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7790.10835939151819993No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7730.10752478773243715No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7530.10474277511322791No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7520.10460367448226744No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7260.10098705807729544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.028.5909081
ACTCTTA400.001930487723.12523
TGTTACG652.6805246E-622.7692316
CGAACTA753.7367317E-722.224
ACATAGG502.7007386E-422.1999992
AGACCGT603.7237867E-521.5833346
ATTGTTA705.0975377E-621.14285714
TAGTTCG806.9526504E-720.81257
GCGGTAA5950.020.5210123
TATACAC3150.019.96825437
CTTATAC9850.019.72081237
CGGTAAT6200.019.69354824
ATACACA1800.019.52777737
GGTAATA6500.019.35384625
GTATCAA12850.019.291832
GTAACGT3650.019.26027526
TAATACG6150.019.25203127
AACGTCA3650.018.75342628
TACGGCT1306.9485395E-1018.530
AGTTCCG902.1499345E-618.532
Produced by FastQC (version 0.11.5)