##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632456.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718904
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.986190089358246	34.0	33.0	34.0	31.0	34.0
2	33.13283692954831	34.0	33.0	34.0	31.0	34.0
3	33.210290942879716	34.0	33.0	34.0	31.0	34.0
4	36.51470432769883	37.0	37.0	37.0	35.0	37.0
5	36.47134805203476	37.0	37.0	37.0	35.0	37.0
6	36.51335226956589	37.0	37.0	37.0	35.0	37.0
7	36.51327854623148	37.0	37.0	37.0	35.0	37.0
8	36.49071364187708	37.0	37.0	37.0	35.0	37.0
9	38.26264007433538	39.0	39.0	39.0	37.0	39.0
10	38.28951153422432	39.0	39.0	39.0	37.0	39.0
11	38.36015239865128	39.0	39.0	39.0	37.0	39.0
12	38.3185752200572	39.0	39.0	39.0	37.0	39.0
13	38.35368839233055	39.0	39.0	39.0	37.0	39.0
14	39.89665657723423	41.0	40.0	41.0	38.0	41.0
15	39.884333652337446	41.0	40.0	41.0	38.0	41.0
16	39.84965308302638	41.0	40.0	41.0	38.0	41.0
17	39.850066211900334	41.0	40.0	41.0	38.0	41.0
18	39.84803116966938	41.0	40.0	41.0	38.0	41.0
19	39.85541602216708	41.0	40.0	41.0	38.0	41.0
20	39.82280387923839	41.0	40.0	41.0	38.0	41.0
21	39.79569733928313	41.0	40.0	41.0	38.0	41.0
22	39.7640199525945	41.0	40.0	41.0	38.0	41.0
23	39.72840323603708	41.0	40.0	41.0	38.0	41.0
24	39.7102019184759	41.0	40.0	41.0	38.0	41.0
25	39.66651597431646	41.0	40.0	41.0	37.0	41.0
26	39.58446607613812	41.0	40.0	41.0	37.0	41.0
27	39.50860198301859	41.0	40.0	41.0	37.0	41.0
28	39.472289763306364	41.0	39.0	41.0	37.0	41.0
29	39.432434650523575	41.0	39.0	41.0	37.0	41.0
30	39.38861516975841	41.0	39.0	41.0	36.0	41.0
31	39.329394467133305	41.0	39.0	41.0	36.0	41.0
32	39.279218087533245	41.0	39.0	41.0	36.0	41.0
33	39.222197956889936	41.0	39.0	41.0	36.0	41.0
34	39.17879021399241	41.0	39.0	41.0	35.0	41.0
35	39.100626787443105	40.0	39.0	41.0	35.0	41.0
36	39.054548312431145	40.0	39.0	41.0	35.0	41.0
37	38.99706080366781	40.0	39.0	41.0	35.0	41.0
38	38.923733906056995	40.0	39.0	41.0	35.0	41.0
39	38.83483608381648	40.0	38.0	41.0	35.0	41.0
40	38.763841903786876	40.0	38.0	41.0	35.0	41.0
41	38.7031634265493	40.0	38.0	41.0	35.0	41.0
42	38.634008156861	40.0	38.0	41.0	35.0	41.0
43	37.9082951270267	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	4.0
18	8.0
19	15.0
20	39.0
21	81.0
22	149.0
23	266.0
24	386.0
25	673.0
26	861.0
27	1204.0
28	1783.0
29	2385.0
30	3225.0
31	4308.0
32	5783.0
33	7554.0
34	11378.0
35	18282.0
36	29170.0
37	55836.0
38	152223.0
39	423286.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.450307690595686	16.26948799839756	12.16268096992088	28.117523341085874
2	21.524709836083815	18.80404059512814	31.787554388346706	27.883695180441336
3	21.54418392441828	19.390210653995528	28.388769571458777	30.676835850127414
4	15.954425069272112	15.365472997785517	33.01010983385821	35.66999209908416
5	17.731018327899132	32.383461491381325	32.620767167799876	17.264753012919666
6	35.392486340318044	32.80883122085842	14.875282374280848	16.923400064542694
7	30.944187262833424	27.203214893782757	19.320938539777217	22.531659303606602
8	27.72553776303929	30.579326307824132	19.40245150952005	22.292684419616528
9	27.951437187719087	12.997841158207493	17.327070095589953	41.72365155848347
10	19.592880273304917	23.017120505658614	29.457897021020884	27.932102200015578
11	39.61905901205168	19.2495799160945	18.708895763551183	22.422465308302638
12	23.350962019963724	23.35638694457118	26.39476202664055	26.897889008824542
13	34.88059601838354	17.56952249535404	20.40550615937594	27.144375326886482
14	24.354016669819615	19.40565082403214	22.55864482601293	33.68168768013532
15	29.49142027308236	23.860487631171896	19.79485438945951	26.853237706286237
16	26.26470293669252	23.418704027241468	22.279191658413364	28.03740137765265
17	27.081919143585235	23.268197144542246	21.785523519134685	27.864360192737834
18	26.703982729265658	21.49216028843907	23.294208962531854	28.509648019763418
19	28.2820793875121	22.16109522272793	22.96537507094132	26.591450318818644
20	29.358022767991276	21.154841258359948	22.475323548067614	27.011812425581162
21	27.816787776949358	22.077634844151653	21.892074602450396	28.213502776448596
22	28.384735653160924	22.676184859174523	21.17723645994458	27.761843027719973
23	28.417006999543748	22.059134460233913	22.11588751766578	27.40797102255656
24	28.286669708333793	21.687179373045637	21.955921792061247	28.07022912655932
25	28.303639985310973	22.02797591889877	22.52734718404683	27.141036911743434
26	28.036427673235924	22.57046837964457	22.14927166909629	27.243832278023213
27	27.458186350333285	21.912661495832545	23.280577100697727	27.348575053136443
28	26.711494163337523	22.77564181031125	22.05231852931685	28.460545497034374
29	27.705368171550028	23.22104203064665	22.119643234701712	26.95394656310161
30	27.315608203598813	21.877886338092427	23.48533322947153	27.32117222883723
31	27.823742808497382	22.146767857739004	22.545847567964568	27.48364176579905
32	25.94296317728097	22.102116555200695	22.830308358278714	29.124611909239622
33	26.46361683896598	21.701089436141682	23.955076060225007	27.880217664667327
34	26.666008257013452	21.446396180853075	23.911537562734384	27.976057999399085
35	26.203081357177037	22.56434795188231	24.045352369718348	27.187218321222307
36	26.458191914358526	21.349582141704595	24.691474800529694	27.500751143407186
37	26.600491859831077	20.795822529850998	24.69940353649444	27.904282073823488
38	25.7293046081257	20.287687924952426	25.860198301859498	28.122809165062375
39	25.15593180730668	19.536683618396893	27.155086075470436	28.15229849882599
40	24.920156237828696	20.323019485216385	27.75600080121963	27.000823475735285
41	23.37753024047717	19.841035798938382	28.6430455248545	28.138388435729944
42	21.979290698062606	20.752840434884213	29.453863102723034	27.814005764330147
43	21.424696482423244	21.018522640018695	28.969236504456784	28.587544373101277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	7.0
2	4.0
3	18.5
4	33.0
5	33.0
6	39.5
7	46.0
8	50.0
9	54.0
10	71.0
11	88.0
12	88.0
13	217.0
14	346.0
15	584.5
16	823.0
17	808.0
18	793.0
19	793.0
20	953.0
21	1113.0
22	947.0
23	781.0
24	954.5
25	1128.0
26	1128.0
27	1351.0
28	1574.0
29	2079.5
30	2585.0
31	3418.0
32	4251.0
33	4251.0
34	5799.0
35	7347.0
36	8720.5
37	10094.0
38	13471.0
39	16848.0
40	16848.0
41	20239.0
42	23630.0
43	28133.5
44	32637.0
45	40899.5
46	49162.0
47	49162.0
48	55633.5
49	62105.0
50	68919.5
51	75734.0
52	77637.5
53	79541.0
54	79541.0
55	75345.0
56	71149.0
57	68074.0
58	64999.0
59	61303.0
60	57607.0
61	57607.0
62	52955.0
63	48303.0
64	40844.0
65	33385.0
66	28881.0
67	24377.0
68	24377.0
69	21034.5
70	17692.0
71	14869.0
72	12046.0
73	9758.0
74	7470.0
75	7470.0
76	6028.5
77	4587.0
78	3689.0
79	2791.0
80	2265.5
81	1740.0
82	1740.0
83	1377.0
84	1014.0
85	785.0
86	556.0
87	411.5
88	267.0
89	267.0
90	198.0
91	129.0
92	86.0
93	43.0
94	29.0
95	15.0
96	15.0
97	10.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	718904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.29110624915053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92889171912347	45.7924569847556
2	7.316767236712071	7.798372404238531
3	2.2073865741173107	3.5290221736270206
4	1.1003073817530926	2.3454639035091476
5	0.6678010486171603	1.779392831757562
6	0.42817489908005496	1.3690748424056711
7	0.322460098547048	1.2028978760947602
8	0.24621487249031787	1.0496850344002064
9	0.1789270943189281	0.8581700514781598
>10	1.3192368922346431	13.768361213649822
>50	0.1631642112055234	5.9223817224475725
>100	0.11174903475427689	11.199186865511177
>500	0.007869650334797734	2.6901981231243477
>1k	0.0010492867113063645	0.695335973000442
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1580	0.21977899691753003	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1237	0.17206748049809154	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1082	0.1505068826992199	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1075	0.14953317828249668	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	962	0.13381480698396447	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	843	0.1172618318996695	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	837	0.11642722811390671	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	779	0.10835939151819993	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	773	0.10752478773243715	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	753	0.10474277511322791	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	752	0.10460367448226744	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	726	0.10098705807729544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3910063096046204E-4	0.0
5	0.0	0.0	0.0	1.3910063096046204E-4	0.0
6	0.0	0.0	0.0	1.3910063096046204E-4	0.0
7	0.0	0.0	0.0	1.3910063096046204E-4	0.0
8	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
9	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
10	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
11	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
12	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
13	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
14	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
15	2.782012619209241E-4	0.0	0.0	1.3910063096046204E-4	0.0
16	2.782012619209241E-4	0.0	0.0	8.346037857627722E-4	0.0
17	2.782012619209241E-4	0.0	0.0	0.0012519056786441584	0.0
18	2.782012619209241E-4	0.0	0.0	0.0015301069405650825	0.0
19	2.782012619209241E-4	0.0	0.0	0.0019474088334464686	0.0
20	2.782012619209241E-4	0.0	0.0	0.0026429119882487788	0.0
21	2.782012619209241E-4	0.0	0.0	0.004729421452655709	0.0
22	2.782012619209241E-4	0.0	0.0	0.006537729655141716	0.0
23	2.782012619209241E-4	0.0	0.0	0.007650534702825412	0.0
24	2.782012619209241E-4	0.0	0.0	0.010015245429153267	0.0
25	2.782012619209241E-4	0.0	0.0	0.012519056786441583	0.0
26	2.782012619209241E-4	0.0	0.0	0.01850038391774145	0.0
27	2.782012619209241E-4	0.0	0.0	0.04979802588384541	0.0
28	2.782012619209241E-4	0.0	0.0	0.17025917229560553	0.0
29	2.782012619209241E-4	0.0	0.0	0.3471951748773132	0.0
30	2.782012619209241E-4	0.0	0.0	0.5696170837830921	0.0
31	2.782012619209241E-4	0.0	0.0	1.2450897477270957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	880	0.0	28.590908	1
ACTCTTA	40	0.0019304877	23.125	23
TGTTACG	65	2.6805246E-6	22.76923	16
CGAACTA	75	3.7367317E-7	22.2	24
ACATAGG	50	2.7007386E-4	22.199999	2
AGACCGT	60	3.7237867E-5	21.583334	6
ATTGTTA	70	5.0975377E-6	21.142857	14
TAGTTCG	80	6.9526504E-7	20.8125	7
GCGGTAA	595	0.0	20.52101	23
TATACAC	315	0.0	19.968254	37
CTTATAC	985	0.0	19.720812	37
CGGTAAT	620	0.0	19.693548	24
ATACACA	180	0.0	19.527777	37
GGTAATA	650	0.0	19.353846	25
GTATCAA	1285	0.0	19.29183	2
GTAACGT	365	0.0	19.260275	26
TAATACG	615	0.0	19.252031	27
AACGTCA	365	0.0	18.753426	28
TACGGCT	130	6.9485395E-10	18.5	30
AGTTCCG	90	2.1499345E-6	18.5	32
>>END_MODULE