FastQCFastQC Report
Fri 10 Feb 2017
ERR1632454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138383
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4890.3533671043408511No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3560.2572570330170614No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2970.21462173821928995No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2300.1662053864997868No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2130.15392064054110693No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2060.14886221573459166No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2030.14669431938894228No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2000.14452642304329288No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1990.14380379092807644No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1990.14380379092807644No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1980.14308115881285996No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1970.14235852669764348No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1960.14163589458242704No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1710.12357009170201542No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1700.12284745958679895No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1650.11923429901071662No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1570.11345324208898491No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1550.11200797785855197No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1550.11200797785855197No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1520.10984008151290259No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1520.10984008151290259No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1500.10839481728246968No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1480.10694955305203674No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1480.10694955305203674TruSeq Adapter, Index 10 (95% over 21bp)
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1470.10622692093682026No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1450.10478165670638735No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1430.10333639247595441No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1420.10261376036073795No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1420.10261376036073795No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1390.10044586401508857No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1390.10044586401508857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTC200.001836346337.02
TAGGACA352.3732648E-531.7142874
GGTATCA1350.030.148151
TACTGGC250.005479854529.67
CAGTCGG250.005479854529.610
GGTAATA1950.029.41025725
GCGGTAA2000.028.67500123
CGCGGTA2100.028.19047522
GCTTAGG405.9030164E-527.751
GTAATAC2100.027.30952326
TAATACG2050.027.0731727
CGGTAAT2150.026.67441724
CCGCGGT2450.025.67347121
TGAGCAA659.267569E-825.61538536
AATACGG2200.025.22727228
CGTGCCA2450.024.9183710
TCAAAAG451.315011E-424.66666820
TTAGGAC601.3232548E-624.6666683
TTAGCCC451.315011E-424.66666829
TACGGCT451.315011E-424.66666830
Produced by FastQC (version 0.11.5)