FastQCFastQC Report
Fri 10 Feb 2017
ERR1632453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311689
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19900.6384569234076275No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14740.4729072889964035No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11790.37826166467215716No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11420.3663908575535229No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8990.28842852972033023No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8420.2701410701051368No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8240.26436608285823365No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8070.25891192823615844No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7950.255061936738223No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7760.2489661168664919No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7760.2489661168664919No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7660.2457577906182124No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7600.24383279486924467No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC7550.2422286317451049No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7530.241586966495449No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7420.23805780762234152No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7210.23132032250095447No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7200.2309994898761265No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7050.22618700050370724No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6680.21431619338507296No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6490.2082203735133418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6340.20340788414092253No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6320.20276621889126661No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6200.19891622739333117No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6130.1966703990195355No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6000.1924995748967721No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5920.18993291389814848No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5910.18961208127332052No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5820.18672458764986893No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5650.18127043302779372No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5630.18062876777813783No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5280.16939962590915944No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5090.16330380603742833No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC5060.16234130816294448No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4950.15881214928983697No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4920.15784965141535312No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4910.15752881879052516No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4880.1565663209160413No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4830.15496215779190156No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4830.15496215779190156No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4700.15079133366913813No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4660.14950800316982635No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4610.14790384004568655No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4540.14565801167189088No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4500.1443746811725791No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4450.14277051804843932No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG4340.1392413591753318No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4320.13859969392567592No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4300.13795802867602No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4260.1366746981767082No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4170.13378720455325663No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4150.1331455393036007No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4110.1318622088042889No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC4050.12993721305532116No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3950.12672888680704164No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3860.12384139318359005No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3800.12191639743462233No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3760.1206330669353105No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3700.11870807118634279No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3660.11742474068703099No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3640.11678307543737507No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3590.11517891231323531No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3560.11421641443875145No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3480.11164975344012781No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3460.11100808819047192No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3460.11100808819047192No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3450.11068725556564396No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT3330.1068372640677085TruSeq Adapter, Index 7 (95% over 21bp)
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3320.10651643144288056No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3280.10523310094356876No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3280.10523310094356876No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA3280.10523310094356876No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3230.10362893781942899No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3170.10170394207046127No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC3160.10138310944563331No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3150.10106227682080535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCC200.001839878937.07
CTCCACT401.5951173E-632.3752
GGTATCA6500.027.892311
TAACCCT405.926459E-527.7535
AATGTTC556.237151E-726.9090920
ACACAGC358.8548375E-426.428571
CACTGTG358.8548375E-426.428577
CAATGTT601.332186E-624.66666819
CATTGTA451.3201914E-424.6666662
ATACGGC901.4188117E-1024.66666629
GGAGGGC751.36551535E-824.66666633
AACCCTG551.8968956E-523.54545436
GCTATAG551.8968956E-523.54545435
GTTAGAA551.8968956E-523.5454543
CGTTAGA551.8968956E-523.5454542
AAGCTAT551.8968956E-523.54545433
GACCGTT400.001928047923.1257
CTGTGCA400.001928047923.1259
CTCAATG652.672441E-622.7692317
TCAATGT652.672441E-622.7692318
Produced by FastQC (version 0.11.5)