FastQCFastQC Report
Fri 10 Feb 2017
ERR1632452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4418
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT350.7922136713444997RNA PCR Primer, Index 39 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA290.6564056133997285No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA260.5885015844273427No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA250.565866908103214No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.4526935264825713No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT160.362154821186057No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC160.362154821186057RNA PCR Primer, Index 39 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT150.33952014486192844No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT140.3168854685377999No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT140.3168854685377999No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT130.29425079221367134No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC130.29425079221367134No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC130.29425079221367134No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC130.29425079221367134No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG120.2716161158895428No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC120.2716161158895428No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC120.2716161158895428No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA120.2716161158895428No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC110.24898143956541421No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG110.24898143956541421No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG110.24898143956541421No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA110.24898143956541421No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA100.22634676324128564No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC100.22634676324128564No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC100.22634676324128564No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA100.22634676324128564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100.22634676324128564No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC100.22634676324128564No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA100.22634676324128564No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA90.2037120869171571No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT90.2037120869171571No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC90.2037120869171571RNA PCR Primer, Index 39 (95% over 24bp)
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT90.2037120869171571No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG90.2037120869171571No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC90.2037120869171571No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA90.2037120869171571No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG90.2037120869171571No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC80.1810774105930285No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC80.1810774105930285No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA80.1810774105930285No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG80.1810774105930285No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC70.15844273426889996No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.15844273426889996No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG70.15844273426889996No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT70.15844273426889996No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA70.15844273426889996No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT70.15844273426889996No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC70.15844273426889996No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG70.15844273426889996No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA70.15844273426889996No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT70.15844273426889996No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC60.1358080579447714No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA60.1358080579447714No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG60.1358080579447714No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA60.1358080579447714No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA60.1358080579447714No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT60.1358080579447714No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC60.1358080579447714No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA60.1358080579447714No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT60.1358080579447714No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA60.1358080579447714No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG60.1358080579447714No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.1358080579447714No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG60.1358080579447714No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA60.1358080579447714No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG50.11317338162064282No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA50.11317338162064282No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC50.11317338162064282No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG50.11317338162064282No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC50.11317338162064282No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.11317338162064282No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACT50.11317338162064282No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC50.11317338162064282No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT50.11317338162064282No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC50.11317338162064282No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA50.11317338162064282No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA50.11317338162064282No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG50.11317338162064282No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC50.11317338162064282No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA50.11317338162064282No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG50.11317338162064282No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG50.11317338162064282No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG50.11317338162064282No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA50.11317338162064282No Hit
GGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTAC50.11317338162064282No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC50.11317338162064282No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT50.11317338162064282No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC50.11317338162064282No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG50.11317338162064282No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG50.11317338162064282No Hit
CAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAAC50.11317338162064282No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.11317338162064282No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers