FastQCFastQC Report
Fri 10 Feb 2017
ERR1632450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19196
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1130.5886643050635549No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1100.5730360491769119No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA670.3490310481350281No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA620.32298395499062305No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA590.30735569910398No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT540.2813086059595749No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT540.2813086059595749TruSeq Adapter, Index 1 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT530.2760991873306939No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT520.27088976870181286No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC520.27088976870181286No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT470.24484267555740777No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA460.2396332569285268No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA450.23442383829964578No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT450.23442383829964578No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA440.22921441967076475No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA440.22921441967076475No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT430.22400500104188373No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA430.22400500104188373No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA420.2187955824130027No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC420.2187955824130027No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC410.2135861637841217No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG410.2135861637841217No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA390.20316732652635966No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG380.19795790789747864No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT380.19795790789747864No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA380.19795790789747864No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC370.19274848926859764No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG360.18753907063971662No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA360.18753907063971662No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC340.17712023338195457No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG320.16670139612419255No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG320.16670139612419255No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC320.16670139612419255No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC310.16149197749531152No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC310.16149197749531152No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC310.16149197749531152No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT300.1562825588664305No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA290.15107314023754947No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA290.15107314023754947No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC280.14586372160866848No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC280.14586372160866848No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC280.14586372160866848No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC260.13544488435090643No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC260.13544488435090643No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT250.13023546572202543No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA250.13023546572202543No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA250.13023546572202543No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA250.13023546572202543No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC250.13023546572202543No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA250.13023546572202543No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA250.13023546572202543No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC250.13023546572202543No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA250.13023546572202543No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA250.13023546572202543No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT250.13023546572202543No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG240.1250260470931444No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG230.1198166284642634No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT230.1198166284642634No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG230.1198166284642634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230.1198166284642634No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC230.1198166284642634No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC220.11460720983538238RNA PCR Primer, Index 1 (95% over 22bp)
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG220.11460720983538238No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC220.11460720983538238No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG220.11460720983538238No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT210.10939779120650135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210.10939779120650135No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG210.10939779120650135No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC210.10939779120650135No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC210.10939779120650135No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC210.10939779120650135No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC210.10939779120650135No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA210.10939779120650135No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG210.10939779120650135No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG200.10418837257762034No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT200.10418837257762034No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC200.10418837257762034No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC200.10418837257762034No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT200.10418837257762034No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC200.10418837257762034No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA200.10418837257762034No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA200.10418837257762034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA355.256061E-737.023
CGCGGTA355.256061E-737.022
CGGTAAT355.256061E-737.024
GGTAATA355.256061E-737.025
CAGCAGC459.888572E-832.8888915
GCAGCCG459.888572E-832.8888917
CAGCCGC459.888572E-832.8888918
CCAGCAG502.4925794E-729.614
AACTCCG502.4925794E-729.65
TAACTCC502.4925794E-729.64
CTAACTC502.4925794E-729.63
GCTAACT502.4925794E-729.62
TGCCAGC502.4925794E-729.612
CGTGCCA502.4925794E-729.610
GGCTAAC502.4925794E-729.61
TCCGTGC502.4925794E-729.68
AGCCGCG502.4925794E-729.619
CTCCGTG502.4925794E-729.67
GTGCCAG502.4925794E-729.611
TAATACG453.7447517E-628.77777927
Produced by FastQC (version 0.11.5)