FastQCFastQC Report
Fri 10 Feb 2017
ERR1632449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258573
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA41930.3331550891366651No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA33260.26426754745255143No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA25090.19935275903741775No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA23980.1905332467802821No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20660.16415416507425473No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19940.1584334003669235No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18910.15024952863282465No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18840.14969334317516744No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT18080.1436547582063178No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT18040.1433369379447994No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17890.14214511196410537No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17170.13642434725677416No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC17120.13602707192987615No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17030.13531197634145972No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16730.1329283243800717No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA16530.13133922307247972No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16070.12768429006501808No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15780.12538009316900967No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT15680.12458554251521366No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC15430.12259916588072364No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15190.12069224431161324No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC15170.12053333418085403No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA14170.11258782764289398No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13950.11083981620454277No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC13500.10726433826246075No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13460.10694651800094233No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC13390.10639033254328514No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12940.10281485460120311No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12940.10281485460120311No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12720.10106684316285189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.029.1023391
CCCTAAT400.001931288523.1251
ATACGGC3650.021.28767229
GTATCAA23500.020.9404242
GCGGTAA16550.020.67975623
CGCGGTA16900.020.36094522
GGTAATA16850.019.98219725
CTTATAC16000.019.1937537
TAATACG16700.019.05389227
CGTGCCA18300.019.00546510
CCGCGGT18300.019.00546521
CGGTAAT17950.018.7576624
AGCCGCG18350.018.75204519
ATTAGAC500.007035244318.53
CTAAGTC1409.458745E-1118.534
TACGGCT4100.018.530
TTAAGGT500.007035244318.54
TCTAAAG609.2374795E-418.52
TCTCGCG801.6168691E-518.520
ATACACA3200.018.537
Produced by FastQC (version 0.11.5)