FastQCFastQC Report
Fri 10 Feb 2017
ERR1632448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8825
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA520.5892351274787535No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA380.43059490084985835No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT370.4192634560906515RNA PCR Primer, Index 46 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA360.40793201133144474No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA270.3059490084985836No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA250.28328611898017No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA250.28328611898017No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT230.26062322946175637No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC230.26062322946175637No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC210.23796033994334279No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC200.22662889518413595No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA190.21529745042492918No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT180.20396600566572237No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA180.20396600566572237No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC180.20396600566572237No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC170.1926345609065156No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT170.1926345609065156No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC170.1926345609065156No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC170.1926345609065156No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT170.1926345609065156No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC160.1813031161473088No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC160.1813031161473088No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG160.1813031161473088No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG160.1813031161473088No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA160.1813031161473088No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT150.16997167138810199No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC150.16997167138810199No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC150.16997167138810199No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG150.16997167138810199No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA150.16997167138810199No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA150.16997167138810199No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG140.15864022662889518No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC140.15864022662889518No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT140.15864022662889518No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC130.14730878186968838No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT130.14730878186968838No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA130.14730878186968838No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA130.14730878186968838No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT130.14730878186968838No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG120.13597733711048157No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT120.13597733711048157No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT120.13597733711048157No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT120.13597733711048157No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT120.13597733711048157No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC110.12464589235127478No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC110.12464589235127478No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC110.12464589235127478No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA110.12464589235127478No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT110.12464589235127478No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA110.12464589235127478No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC110.12464589235127478No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC110.12464589235127478No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC110.12464589235127478No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG110.12464589235127478No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC110.12464589235127478No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT110.12464589235127478No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA100.11331444759206798No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA100.11331444759206798No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC100.11331444759206798No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA100.11331444759206798No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC100.11331444759206798No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG100.11331444759206798No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC100.11331444759206798No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG100.11331444759206798No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT100.11331444759206798No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG100.11331444759206798No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA90.10198300283286119No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA90.10198300283286119No Hit
ATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGAT90.10198300283286119No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG90.10198300283286119No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACC90.10198300283286119No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.10198300283286119No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT90.10198300283286119No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA90.10198300283286119No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA90.10198300283286119No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC90.10198300283286119No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC90.10198300283286119No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGC90.10198300283286119No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG90.10198300283286119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA250.00520874229.61
GTCTCTT854.65263E-415.23529337
TGTCTCT1000.001594254612.95000136
CTGTCTC1250.00847664410.36000135
Produced by FastQC (version 0.11.5)