FastQCFastQC Report
Fri 10 Feb 2017
ERR1632447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8337
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT290.347846947343169RNA PCR Primer, Index 27 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA240.2878733357322778No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA220.2638838910879213No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG220.2638838910879213No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.23989444644356483No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.22789972412138657No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA180.21590500179920835No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA170.20391027947703008No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT160.19191555715485187No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA160.19191555715485187No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA150.17992083483267363No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT140.16792611251049538No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC140.16792611251049538No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC140.16792611251049538RNA PCR Primer, Index 27 (95% over 24bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG130.15593139018831714No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC130.15593139018831714No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA130.15593139018831714No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC130.15593139018831714No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA130.15593139018831714No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA130.15593139018831714No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG120.1439366678661389No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT120.1439366678661389No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC120.1439366678661389No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC120.1439366678661389RNA PCR Primer, Index 27 (95% over 21bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT120.1439366678661389No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT110.13194194554396066No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG110.13194194554396066No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA110.13194194554396066No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG100.11994722322178242No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG100.11994722322178242No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT100.11994722322178242No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT100.11994722322178242No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG100.11994722322178242No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA100.11994722322178242No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT100.11994722322178242No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA100.11994722322178242No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC90.10795250089960418No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC90.10795250089960418No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA90.10795250089960418No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC90.10795250089960418No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA90.10795250089960418No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC90.10795250089960418No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC90.10795250089960418No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT90.10795250089960418No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT90.10795250089960418No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA90.10795250089960418No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC90.10795250089960418No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC90.10795250089960418No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC90.10795250089960418No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC90.10795250089960418No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC90.10795250089960418No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG90.10795250089960418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATAC250.00519161329.610
AAATACT250.00519161329.611
GCTAAAT250.00519161329.68
AAGGCTA250.00519161329.65
AGGCTAA250.00519161329.66
CTAAATA250.00519161329.69
GGCTAAA250.00519161329.67
AATACTC250.00519161329.612
ATACTCC250.00519161329.613
CTGTCTC1102.0287407E-413.45454537
Produced by FastQC (version 0.11.5)