##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632447.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8337
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99376274439247	34.0	33.0	34.0	31.0	34.0
2	33.12042701211467	34.0	33.0	34.0	31.0	34.0
3	33.17284394866259	34.0	33.0	34.0	31.0	34.0
4	36.48446683459278	37.0	37.0	37.0	35.0	37.0
5	36.449442245412015	37.0	37.0	37.0	35.0	37.0
6	36.50353844308504	37.0	37.0	37.0	35.0	37.0
7	36.512894326496344	37.0	37.0	37.0	35.0	37.0
8	36.45567950101955	37.0	37.0	37.0	35.0	37.0
9	38.211706848986445	39.0	39.0	39.0	37.0	39.0
10	38.24193354923833	39.0	39.0	39.0	37.0	39.0
11	38.31030346647475	39.0	39.0	39.0	37.0	39.0
12	38.2824757106873	39.0	39.0	39.0	37.0	39.0
13	38.34400863620007	39.0	39.0	39.0	37.0	39.0
14	39.836991723641596	41.0	40.0	41.0	38.0	41.0
15	39.83951061532925	41.0	40.0	41.0	38.0	41.0
16	39.81959937627444	41.0	40.0	41.0	38.0	41.0
17	39.82583663188197	41.0	40.0	41.0	38.0	41.0
18	39.80748470672904	41.0	40.0	41.0	38.0	41.0
19	39.84154971812403	41.0	40.0	41.0	38.0	41.0
20	39.801727240014394	41.0	40.0	41.0	38.0	41.0
21	39.774859062012716	41.0	40.0	41.0	38.0	41.0
22	39.72280196713446	41.0	40.0	41.0	37.0	41.0
23	39.69701331414178	41.0	40.0	41.0	37.0	41.0
24	39.67818160009596	41.0	40.0	41.0	37.0	41.0
25	39.66234856663068	41.0	40.0	41.0	37.0	41.0
26	39.54360081564112	41.0	40.0	41.0	37.0	41.0
27	39.428811323017875	41.0	39.0	41.0	36.0	41.0
28	39.399304306105314	41.0	39.0	41.0	37.0	41.0
29	39.34184958618208	41.0	39.0	41.0	36.0	41.0
30	39.28391507736596	41.0	39.0	41.0	36.0	41.0
31	39.2554875854624	41.0	39.0	41.0	36.0	41.0
32	39.17356363200192	41.0	39.0	41.0	36.0	41.0
33	39.104713925872616	41.0	39.0	41.0	35.0	41.0
34	39.052296989324695	41.0	39.0	41.0	35.0	41.0
35	38.99484226940147	40.0	39.0	41.0	35.0	41.0
36	38.94758306345208	40.0	39.0	41.0	35.0	41.0
37	38.90931989924433	40.0	39.0	41.0	35.0	41.0
38	38.81012354563992	40.0	38.0	41.0	35.0	41.0
39	38.67674223341729	40.0	38.0	41.0	35.0	41.0
40	38.58030466594698	40.0	38.0	41.0	35.0	41.0
41	38.576826196473554	40.0	38.0	41.0	35.0	41.0
42	38.490824037423536	40.0	38.0	41.0	35.0	41.0
43	37.74235336451961	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	2.0
24	8.0
25	8.0
26	9.0
27	20.0
28	27.0
29	41.0
30	35.0
31	53.0
32	63.0
33	102.0
34	157.0
35	211.0
36	340.0
37	684.0
38	1745.0
39	4830.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.557274799088404	16.888569029626964	12.534484826676263	28.019671344608373
2	22.454120187117667	19.76730238694974	31.40218303946264	26.376394386469954
3	21.49454240134341	19.047619047619047	28.811323017872137	30.64651553316541
4	15.473191795609932	15.437207628643396	34.916636679860865	34.17296389588581
5	17.896125704689936	32.061892767182435	32.82955499580184	17.21242653232578
6	32.781576106513135	34.077006117308386	15.737075686697851	17.40434208948063
7	28.727359961616887	27.503898284754708	19.73131821998321	24.037423533645196
8	27.587861341009955	30.64651553316541	20.019191555715484	21.746431570109152
9	27.25200911598896	12.510495382031905	17.95609931630083	42.2813961856783
10	19.85126544320499	22.3821518531846	28.499460237495498	29.26712246611491
11	38.88688976850186	19.971212666426773	18.4118987645436	22.729998800527767
12	22.058294350485784	23.94146575506777	26.760225500779654	27.24001439366679
13	36.75182919515414	16.70864819479429	19.92323377713806	26.616288832913522
14	23.617608252368957	20.846827395945784	22.526088521050738	33.00947583063452
15	29.890848026868177	24.865059373875496	19.191555715485187	26.05253688377114
16	25.464795489984404	23.209787693414896	22.442125464795488	28.883291351805205
17	27.33597217224421	23.017872136260046	21.33861101115509	28.30754468034065
18	26.388389108792133	21.062732397744995	22.99388269161569	29.554995801847188
19	27.659829674943026	22.80196713446084	22.753988245172124	26.78421494542401
20	30.56255247691016	20.307064891447762	22.298188796929352	26.832193834712726
21	27.527887729399065	22.262204629962817	20.726880172724	29.48302746791412
22	27.791771620486987	22.69401463356123	21.926352404941827	27.587861341009955
23	28.295549958018473	21.650473791531727	21.926352404941827	28.127623845507976
24	27.647834952620848	21.422574067410338	21.782415737075684	29.147175242893127
25	29.327096077725802	21.35060573347727	22.99388269161569	26.32841549718124
26	28.439486625884612	22.430130742473313	22.142257406741034	26.988125224901044
27	26.832193834712726	21.542521290632124	24.88904881851985	26.736236056135297
28	26.772220223101833	22.310183519251527	21.782415737075684	29.135180520570948
29	26.832193834712726	24.09739714525609	21.998320738874895	27.072088281156294
30	25.884610771260647	22.634041021950342	23.881492143456878	27.599856063332133
31	28.079644956219262	22.430130742473313	22.849946023749553	26.640278277557876
32	25.200911598896486	21.674463236176084	22.96989324697133	30.154731917956102
33	25.15293270960777	21.5545160129543	24.805085762264603	28.487465515173323
34	25.788652992683218	22.37015713086242	23.161808804126185	28.679381072328177
35	26.136499940026386	23.67758186397985	23.821518531845985	26.364399664147776
36	27.216024949022433	22.610051577305985	24.193354923833514	25.980568549838072
37	27.86373995442005	20.295070169125584	24.793091039942425	27.048098836511937
38	25.62072688017272	20.786853784334895	25.416816600695697	28.17560273479669
39	25.95657910519372	20.031186278037662	26.208468273959458	27.803766342809165
40	24.445244092599257	21.062732397744995	28.151613290152333	26.34041021950342
41	23.67758186397985	19.44344488425093	29.135180520570948	27.743792731198276
42	21.446563512054695	20.73887489504618	31.12630442605254	26.688257166846586
43	20.498980448602616	21.998320738874895	29.243133021470552	28.259565791051937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	4.0
14	6.0
15	7.0
16	8.0
17	9.0
18	10.0
19	10.0
20	11.5
21	13.0
22	15.0
23	17.0
24	18.5
25	20.0
26	20.0
27	18.0
28	16.0
29	20.0
30	24.0
31	38.0
32	52.0
33	52.0
34	59.0
35	66.0
36	81.5
37	97.0
38	128.0
39	159.0
40	159.0
41	182.0
42	205.0
43	258.0
44	311.0
45	461.0
46	611.0
47	611.0
48	707.0
49	803.0
50	887.0
51	971.0
52	991.5
53	1012.0
54	1012.0
55	929.5
56	847.0
57	861.0
58	875.0
59	797.5
60	720.0
61	720.0
62	622.5
63	525.0
64	420.0
65	315.0
66	275.0
67	235.0
68	235.0
69	195.0
70	155.0
71	126.0
72	97.0
73	80.5
74	64.0
75	64.0
76	50.5
77	37.0
78	27.5
79	18.0
80	15.5
81	13.0
82	13.0
83	12.0
84	11.0
85	11.0
86	11.0
87	9.5
88	8.0
89	8.0
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8337.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.77821758426292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.51741915325731	67.96209667746191
2	5.999062646461491	9.21194674343289
3	2.155913138572098	4.965815041381792
4	1.1560693641618496	3.55043780736476
5	0.4218090923293235	1.6192875134940627
6	0.4218090923293235	1.9431450161928752
7	0.359318856428683	1.9311502938706968
8	0.1562255897516013	0.9595777857742593
9	0.24996094360256207	1.7272400143936668
>10	0.5624121231057647	6.129303106633081
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	29	0.347846947343169	RNA PCR Primer, Index 27 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	24	0.2878733357322778	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	22	0.2638838910879213	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	22	0.2638838910879213	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	20	0.23989444644356483	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	19	0.22789972412138657	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	18	0.21590500179920835	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	17	0.20391027947703008	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	16	0.19191555715485187	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	16	0.19191555715485187	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	15	0.17992083483267363	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	14	0.16792611251049538	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	14	0.16792611251049538	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	14	0.16792611251049538	RNA PCR Primer, Index 27 (95% over 24bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	13	0.15593139018831714	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	13	0.15593139018831714	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	13	0.15593139018831714	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	13	0.15593139018831714	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	13	0.15593139018831714	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	13	0.15593139018831714	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	12	0.1439366678661389	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	12	0.1439366678661389	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	12	0.1439366678661389	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	12	0.1439366678661389	RNA PCR Primer, Index 27 (95% over 21bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	12	0.1439366678661389	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	11	0.13194194554396066	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	11	0.13194194554396066	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	11	0.13194194554396066	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	10	0.11994722322178242	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	10	0.11994722322178242	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	10	0.11994722322178242	No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT	10	0.11994722322178242	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	10	0.11994722322178242	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	10	0.11994722322178242	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	10	0.11994722322178242	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	10	0.11994722322178242	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	9	0.10795250089960418	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	9	0.10795250089960418	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	9	0.10795250089960418	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	9	0.10795250089960418	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	9	0.10795250089960418	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	9	0.10795250089960418	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	9	0.10795250089960418	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	9	0.10795250089960418	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	9	0.10795250089960418	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	9	0.10795250089960418	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	9	0.10795250089960418	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	9	0.10795250089960418	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	9	0.10795250089960418	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	9	0.10795250089960418	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	9	0.10795250089960418	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	9	0.10795250089960418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.011994722322178242	0.0
23	0.0	0.0	0.0	0.011994722322178242	0.0
24	0.0	0.0	0.0	0.011994722322178242	0.0
25	0.0	0.0	0.0	0.023989444644356483	0.0
26	0.0	0.0	0.0	0.023989444644356483	0.0
27	0.0	0.0	0.0	0.035984166966534725	0.0
28	0.0	0.0	0.0	0.16792611251049538	0.0
29	0.0	0.0	0.0	0.3118627803766343	0.0
30	0.0	0.0	0.0	0.5037783375314862	0.0
31	0.0	0.0	0.0	1.0315461197073288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAATAC	25	0.005191613	29.6	10
AAATACT	25	0.005191613	29.6	11
GCTAAAT	25	0.005191613	29.6	8
AAGGCTA	25	0.005191613	29.6	5
AGGCTAA	25	0.005191613	29.6	6
CTAAATA	25	0.005191613	29.6	9
GGCTAAA	25	0.005191613	29.6	7
AATACTC	25	0.005191613	29.6	12
ATACTCC	25	0.005191613	29.6	13
CTGTCTC	110	2.0287407E-4	13.454545	37
>>END_MODULE