FastQCFastQC Report
Fri 10 Feb 2017
ERR1632446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT210.7551240560949299Illumina PCR Primer Index 10 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA120.4314994606256742No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC110.3955411722402014No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA90.3236245954692557No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT80.2876663070837828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50.17979144192736426No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT50.17979144192736426No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC50.17979144192736426No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC50.17979144192736426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1438331535418914No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA40.1438331535418914No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC40.1438331535418914No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT40.1438331535418914No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG40.1438331535418914No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.1438331535418914No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG40.1438331535418914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40.1438331535418914No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG40.1438331535418914No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT40.1438331535418914No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA40.1438331535418914No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC40.1438331535418914No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG40.1438331535418914No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT40.1438331535418914No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC40.1438331535418914Illumina PCR Primer Index 10 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT40.1438331535418914No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA40.1438331535418914No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG30.10787486515641855No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT30.10787486515641855No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC30.10787486515641855No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC30.10787486515641855No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG30.10787486515641855No Hit
CGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGT30.10787486515641855No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACCT30.10787486515641855No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT30.10787486515641855No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT30.10787486515641855No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA30.10787486515641855No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA30.10787486515641855No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACA30.10787486515641855No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.10787486515641855No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30.10787486515641855No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC30.10787486515641855No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA30.10787486515641855No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC30.10787486515641855No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.10787486515641855No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG30.10787486515641855No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT30.10787486515641855No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC30.10787486515641855No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCG30.10787486515641855No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.10787486515641855No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA30.10787486515641855No Hit
GTTCTAGATGCTGTCCGAGGCAGTCCTGCCATCAATGTGGCCG30.10787486515641855No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG30.10787486515641855No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA30.10787486515641855No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA30.10787486515641855No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA30.10787486515641855No Hit
GGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTGCATGGAGCCG30.10787486515641855No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGCTGTCT30.10787486515641855No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG30.10787486515641855No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC30.10787486515641855No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT30.10787486515641855No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG30.10787486515641855No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC30.10787486515641855No Hit
ACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGT30.10787486515641855No Hit
ACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCC30.10787486515641855No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT30.10787486515641855No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG30.10787486515641855No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers