##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632446.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2781
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78389068680331	34.0	31.0	34.0	31.0	34.0
2	32.935634663790005	34.0	33.0	34.0	31.0	34.0
3	32.99856166846458	34.0	33.0	34.0	31.0	34.0
4	36.329377921610934	37.0	37.0	37.0	35.0	37.0
5	36.31175836030205	37.0	37.0	37.0	35.0	37.0
6	36.38583243437613	37.0	37.0	37.0	35.0	37.0
7	36.38870909744696	37.0	37.0	37.0	35.0	37.0
8	36.349874145990654	37.0	37.0	37.0	35.0	37.0
9	38.14527148507731	39.0	39.0	39.0	37.0	39.0
10	38.1787126932758	39.0	39.0	39.0	37.0	39.0
11	38.16073354908306	39.0	39.0	39.0	37.0	39.0
12	38.140956490471055	39.0	39.0	39.0	37.0	39.0
13	38.24847177274362	39.0	39.0	39.0	37.0	39.0
14	39.71988493347717	41.0	40.0	41.0	37.0	41.0
15	39.65659834591873	41.0	40.0	41.0	38.0	41.0
16	39.590435095289465	41.0	40.0	41.0	37.0	41.0
17	39.55231930960086	41.0	40.0	41.0	37.0	41.0
18	39.614886731391586	41.0	39.0	41.0	37.0	41.0
19	39.56526429341963	41.0	40.0	41.0	37.0	41.0
20	39.55195972671701	41.0	40.0	41.0	37.0	41.0
21	39.59618842143114	41.0	40.0	41.0	37.0	41.0
22	39.56706220783891	41.0	39.0	41.0	37.0	41.0
23	39.55016181229774	41.0	40.0	41.0	37.0	41.0
24	39.46997482919813	41.0	39.0	41.0	37.0	41.0
25	39.45091693635383	41.0	39.0	41.0	37.0	41.0
26	39.30780294857965	41.0	39.0	41.0	36.0	41.0
27	39.20676015821647	40.0	39.0	41.0	36.0	41.0
28	39.17943185904351	41.0	39.0	41.0	35.0	41.0
29	39.08378281193815	40.0	39.0	41.0	36.0	41.0
30	39.058971592952176	41.0	39.0	41.0	35.0	41.0
31	38.93599424667386	40.0	39.0	41.0	35.0	41.0
32	38.993527508090615	40.0	39.0	41.0	35.0	41.0
33	38.816253146350235	40.0	39.0	41.0	35.0	41.0
34	38.745415318230854	40.0	39.0	41.0	35.0	41.0
35	38.668824163969795	40.0	38.0	41.0	35.0	41.0
36	38.56886012225818	40.0	38.0	41.0	35.0	41.0
37	38.62099964041712	40.0	38.0	41.0	35.0	41.0
38	38.5131247752607	40.0	38.0	41.0	35.0	41.0
39	38.37935994246674	40.0	38.0	41.0	34.0	41.0
40	38.28694714131608	40.0	37.0	41.0	34.0	41.0
41	38.21826681049982	40.0	38.0	41.0	34.0	41.0
42	38.12189859762675	40.0	37.0	41.0	34.0	41.0
43	37.482560230133046	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	0.0
23	1.0
24	6.0
25	3.0
26	4.0
27	8.0
28	9.0
29	15.0
30	22.0
31	29.0
32	31.0
33	44.0
34	65.0
35	74.0
36	130.0
37	234.0
38	610.0
39	1495.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.01294498381877	15.390147428982381	12.837108953613807	27.759798633585042
2	22.330097087378643	19.093851132686083	30.312837108953616	28.26321467098166
3	21.898597626752967	18.230852211434737	29.054297015462065	30.816253146350235
4	17.691477885652642	14.850773103200288	32.7220424307803	34.735706580366774
5	18.590435095289465	32.5782092772384	31.67925206760158	17.15210355987055
6	34.66379000359583	33.58504135203164	14.167565623876305	17.583603020496223
7	32.1826681049982	28.0834232290543	17.763394462423587	21.970514203523912
8	27.615965480043148	29.306005034160375	20.747932398417834	22.330097087378643
9	25.602301330456672	11.686443725278677	18.194893923049264	44.51636102121539
10	19.777058612010066	22.761596548004313	27.508090614886733	29.953254225098885
11	39.26645091693635	19.705142035239124	17.51168644372528	23.516720604099245
12	22.330097087378643	21.754764473211075	26.57317511686444	29.341963322545844
13	37.001078748651565	16.43293779216109	18.4106436533621	28.155339805825243
14	26.033800791082346	18.626393383674937	21.610931319669184	33.728874505573536
15	30.276878820568143	22.86947141316073	19.92089176555196	26.932758000719165
16	28.0834232290543	22.97734627831715	20.460266091334052	28.478964401294498
17	26.681049982020856	24.34376123696512	20.711974110032365	28.26321467098166
18	26.860841423948216	20.927723840345198	22.905429701546208	29.306005034160375
19	30.49262855088098	20.891765551959725	22.07838906868033	26.537216828478964
20	31.715210355987054	20.855807263574253	20.927723840345198	26.50125854009349
21	27.687882056814097	20.604099244875947	22.294138798993167	29.413879899316793
22	29.377921610931317	21.215390147428984	19.92089176555196	29.48579647608774
23	29.629629629629626	21.107515282272562	22.07838906868033	27.184466019417474
24	27.97554836389788	20.999640417116144	21.035598705501616	29.989212513484357
25	29.018338727076593	20.06472491909385	21.898597626752967	29.018338727076593
26	29.737504494786048	20.963682128730674	21.503056454512766	27.795756921970515
27	26.033800791082346	21.718806184825603	25.278676734987414	26.968716289104638
28	27.544048903272206	22.72563825961884	20.999640417116144	28.730672419992807
29	26.06975907946782	23.948220064724918	22.22222222222222	27.759798633585042
30	26.71700827040633	21.970514203523912	24.05609492988134	27.256382596188423
31	28.98238043869112	21.251348435814453	21.035598705501616	28.730672419992807
32	27.148507731032	20.496224379719525	22.54584681769148	29.809421071556997
33	27.939590075512406	19.884933477166484	23.948220064724918	28.227256382596188
34	28.155339805825243	20.42430780294858	22.114347357065803	29.306005034160375
35	27.07659115426106	22.402013664149585	23.229054297015463	27.292340884573896
36	27.364257461344838	20.963682128730674	24.883135562747213	26.788924847177277
37	28.62279755483639	20.604099244875947	23.480762315713772	27.292340884573896
38	26.7529665587918	20.532182668104998	24.775260697590795	27.939590075512406
39	24.020136641495863	20.02876663070838	26.249550521395182	29.701546206400575
40	23.98417835311039	19.741100323624593	28.119381517439766	28.155339805825243
41	24.739302409205322	20.460266091334052	27.220424307802947	27.58000719165768
42	21.071556993887093	19.92089176555196	29.557713052858688	29.449838187702266
43	20.891765551959725	18.985976267529665	31.175836030204962	28.946422150305644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	3.0
10	2.0
11	1.0
12	1.0
13	2.0
14	3.0
15	4.0
16	5.0
17	6.5
18	8.0
19	8.0
20	7.5
21	7.0
22	6.0
23	5.0
24	6.0
25	7.0
26	7.0
27	5.5
28	4.0
29	9.0
30	14.0
31	15.5
32	17.0
33	17.0
34	19.0
35	21.0
36	23.0
37	25.0
38	39.0
39	53.0
40	53.0
41	61.0
42	69.0
43	86.0
44	103.0
45	129.0
46	155.0
47	155.0
48	185.5
49	216.0
50	239.0
51	262.0
52	286.0
53	310.0
54	310.0
55	279.5
56	249.0
57	246.5
58	244.0
59	260.5
60	277.0
61	277.0
62	251.5
63	226.0
64	188.5
65	151.0
66	124.0
67	97.0
68	97.0
69	87.5
70	78.0
71	79.5
72	81.0
73	56.5
74	32.0
75	32.0
76	26.0
77	20.0
78	20.0
79	20.0
80	13.0
81	6.0
82	6.0
83	8.0
84	10.0
85	5.0
86	0.0
87	1.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2781.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.46422150305645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.72990353697749	83.8547285149227
2	3.617363344051447	6.472491909385113
3	1.607717041800643	4.314994606256742
4	0.6832797427652734	2.445163610212154
5	0.1607717041800643	0.7191657677094571
6	0.0	0.0
7	0.0	0.0
8	0.04019292604501608	0.2876663070837828
9	0.04019292604501608	0.3236245954692557
>10	0.12057877813504825	1.5821646889608056
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	21	0.7551240560949299	Illumina PCR Primer Index 10 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	12	0.4314994606256742	No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	11	0.3955411722402014	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	9	0.3236245954692557	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	8	0.2876663070837828	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.17979144192736426	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	5	0.17979144192736426	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	5	0.17979144192736426	No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	5	0.17979144192736426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.1438331535418914	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4	0.1438331535418914	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	4	0.1438331535418914	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	4	0.1438331535418914	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	4	0.1438331535418914	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	4	0.1438331535418914	No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG	4	0.1438331535418914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.1438331535418914	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	4	0.1438331535418914	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	4	0.1438331535418914	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	4	0.1438331535418914	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	4	0.1438331535418914	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	4	0.1438331535418914	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	4	0.1438331535418914	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	4	0.1438331535418914	Illumina PCR Primer Index 10 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	4	0.1438331535418914	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	4	0.1438331535418914	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	3	0.10787486515641855	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	3	0.10787486515641855	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	3	0.10787486515641855	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	3	0.10787486515641855	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	3	0.10787486515641855	No Hit
CGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGT	3	0.10787486515641855	No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACCT	3	0.10787486515641855	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	3	0.10787486515641855	No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT	3	0.10787486515641855	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	3	0.10787486515641855	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3	0.10787486515641855	No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACA	3	0.10787486515641855	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	3	0.10787486515641855	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	3	0.10787486515641855	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	3	0.10787486515641855	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	3	0.10787486515641855	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	3	0.10787486515641855	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	3	0.10787486515641855	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	3	0.10787486515641855	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	3	0.10787486515641855	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	3	0.10787486515641855	No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCG	3	0.10787486515641855	No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG	3	0.10787486515641855	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3	0.10787486515641855	No Hit
GTTCTAGATGCTGTCCGAGGCAGTCCTGCCATCAATGTGGCCG	3	0.10787486515641855	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	3	0.10787486515641855	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	3	0.10787486515641855	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	3	0.10787486515641855	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA	3	0.10787486515641855	No Hit
GGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTGCATGGAGCCG	3	0.10787486515641855	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGCTGTCT	3	0.10787486515641855	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	3	0.10787486515641855	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	3	0.10787486515641855	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	3	0.10787486515641855	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	3	0.10787486515641855	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	3	0.10787486515641855	No Hit
ACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGT	3	0.10787486515641855	No Hit
ACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCC	3	0.10787486515641855	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	3	0.10787486515641855	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	3	0.10787486515641855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.10787486515641856	0.0
29	0.0	0.0	0.0	0.17979144192736426	0.0
30	0.0	0.0	0.0	0.32362459546925565	0.0
31	0.0	0.0	0.0	0.43149946062567424	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE