FastQCFastQC Report
Fri 10 Feb 2017
ERR1632444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74058
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3200.4320937643468633No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2400.32407032326014745No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1810.24440303545869455No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1750.23630127737719084No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1690.22819951929568716TruSeq Adapter, Index 12 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1680.22684922628210322No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1590.21469658915984768No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1540.20794512409192792No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1520.20524453806476006No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1510.2038942450511761No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1460.19714277998325636No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1380.1863404358745848No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1280.17283750573874532No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1270.17148721272516138No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1270.17148721272516138No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1270.17148721272516138No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1240.16743633368440952No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1180.15933457560290581No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1160.15663398957573793No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1160.15663398957573793No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1150.155283696562154No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1040.14043047341273057No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1030.13908018039914663No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1030.13908018039914663No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1030.13908018039914663No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1010.13637959437197872No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1010.13637959437197872No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC990.13367900834481083No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA980.13232871533122686No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA970.13097842231764292No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC970.13097842231764292No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC960.12962812930405898No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG960.12962812930405898No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG960.12962812930405898No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC940.12692754327689107No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA900.12152637122255529No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC850.11477490615463555No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC850.11477490615463555No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC830.11207432012746767No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC810.10937373410029975No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT810.10937373410029975No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG810.10937373410029975No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC790.10667314807313187No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC780.10532285505954792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG308.207362E-637.05
GTATACG308.207362E-637.04
GACGTAT352.3584864E-531.7142851
ACGGTGT352.3584864E-531.7142858
ATGGGCA250.005463608529.65
CTCTATG250.005463608529.61
GGTATCA1500.029.61
CCCGGTG405.866553E-527.7523
TGCCGGA405.866553E-527.7528
ACGTATA405.866553E-527.752
GACGCCT405.866553E-527.7515
ATACGGT405.866553E-527.756
CGTATAC405.866553E-527.753
GCCCGGT405.866553E-527.7522
TTATACA358.790948E-426.4285737
CGCCCTA508.942068E-625.90000216
CCGCCCT508.942068E-625.90000215
GTCCCGC508.942068E-625.90000212
CGTGTCC508.942068E-625.9000029
TGCCCGG451.3069535E-424.66666821
Produced by FastQC (version 0.11.5)