##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632444.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74058
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59028059088822	34.0	31.0	34.0	31.0	34.0
2	32.770166626157874	34.0	31.0	34.0	31.0	34.0
3	32.844068162791324	34.0	31.0	34.0	31.0	34.0
4	36.31108050446947	37.0	37.0	37.0	35.0	37.0
5	36.169718328877366	37.0	35.0	37.0	35.0	37.0
6	36.17668584082746	37.0	36.0	37.0	35.0	37.0
7	36.20290853115126	37.0	36.0	37.0	35.0	37.0
8	36.17119014826217	37.0	36.0	37.0	35.0	37.0
9	37.859718058818764	39.0	38.0	39.0	35.0	39.0
10	37.85401982230144	39.0	38.0	39.0	35.0	39.0
11	37.92707067433633	39.0	38.0	39.0	35.0	39.0
12	37.86314780307327	39.0	38.0	39.0	35.0	39.0
13	37.92997380431554	39.0	38.0	39.0	35.0	39.0
14	39.274163493478085	40.0	39.0	41.0	36.0	41.0
15	39.255434929379675	40.0	39.0	41.0	36.0	41.0
16	39.213562343028435	40.0	39.0	41.0	36.0	41.0
17	39.2155472737584	40.0	39.0	41.0	36.0	41.0
18	39.22919873612574	40.0	39.0	41.0	36.0	41.0
19	39.2277539226012	40.0	39.0	41.0	36.0	41.0
20	39.218045315833535	40.0	39.0	41.0	36.0	41.0
21	39.17218936509222	40.0	39.0	41.0	36.0	41.0
22	39.11239839045073	40.0	39.0	41.0	36.0	41.0
23	39.07448216262929	40.0	39.0	41.0	36.0	41.0
24	39.03834832158579	40.0	38.0	41.0	36.0	41.0
25	38.99080450457749	40.0	38.0	41.0	36.0	41.0
26	38.890707283480516	40.0	38.0	41.0	35.0	41.0
27	38.745901860703775	40.0	38.0	41.0	35.0	41.0
28	38.70718896000432	40.0	38.0	41.0	35.0	41.0
29	38.66323692241216	40.0	38.0	41.0	35.0	41.0
30	38.61208782305761	40.0	38.0	41.0	35.0	41.0
31	38.551041075913474	40.0	38.0	41.0	35.0	41.0
32	38.4985686894056	40.0	38.0	41.0	35.0	41.0
33	38.41656539469065	40.0	38.0	41.0	35.0	41.0
34	38.3913419211969	40.0	38.0	41.0	34.0	41.0
35	38.287936482216644	40.0	38.0	41.0	34.0	41.0
36	38.226093062194494	40.0	38.0	41.0	34.0	41.0
37	38.172472926625076	40.0	38.0	41.0	34.0	41.0
38	38.10086688811472	40.0	37.0	41.0	34.0	41.0
39	37.98472818601637	40.0	37.0	41.0	34.0	41.0
40	37.894434092198004	40.0	37.0	41.0	33.0	41.0
41	37.846404169704826	40.0	37.0	41.0	33.0	41.0
42	37.75836506521915	40.0	37.0	41.0	33.0	41.0
43	36.99520645980178	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	0.0
19	3.0
20	6.0
21	17.0
22	29.0
23	48.0
24	80.0
25	89.0
26	146.0
27	198.0
28	313.0
29	374.0
30	508.0
31	692.0
32	930.0
33	1271.0
34	1830.0
35	3012.0
36	5174.0
37	10300.0
38	23447.0
39	25588.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.632801317885985	16.607253774068973	12.861540954387102	27.898403953657947
2	22.31764292851549	19.88981609009155	31.04728726133571	26.745253720057256
3	21.951713521834236	19.26057954576143	29.289205757649412	29.49850117475492
4	15.794377379891436	16.29938696697183	34.5053876691242	33.400847984012536
5	17.57811445083583	32.71084825407114	32.385427637797406	17.325609657295633
6	32.58257041778066	34.29339166599152	15.872694374679305	17.25134354154852
7	28.938129574117582	27.701261173674684	19.45907261875827	23.901536633449457
8	27.046369062086473	30.060223068405843	20.714845121391342	22.17856274811634
9	28.10499878473629	12.17964298252721	17.439034270436686	42.27632396229982
10	20.219287585406036	22.367603770018093	28.208971346782253	29.20413729779362
11	40.27654000918199	18.688055308001836	18.238407734478383	22.79699694833779
12	22.39595992330336	23.56666396608064	26.63587998595695	27.401496124659054
13	36.8940560101542	17.290502038942453	19.869561694887793	25.945880256015556
14	24.060871209052365	20.12611736746874	22.69707526533258	33.11593615814632
15	30.51662210699722	23.581517189230063	19.19171460206865	26.71014610170407
16	24.95881606308569	24.006859488509004	22.306840584406817	28.727483863998486
17	27.582435388479297	23.46674228307543	20.848524129736155	28.102298198709118
18	27.084177266466824	20.267898133895056	22.64036295876205	30.00756164087607
19	28.932728402063248	22.031380809635692	22.583650652191526	26.452240136109534
20	30.994625833805934	19.812849388317264	21.789678360204164	27.402846417672635
21	28.285938048556535	20.89848497123876	21.495314483242865	29.320262496961842
22	28.632963353047614	22.505333657403657	20.762105376866778	28.099597612681954
23	29.210888762861543	21.348132544762212	21.639795835696347	27.8011828566799
24	28.466877312376788	20.58791757811445	22.30413999837965	28.641065111129116
25	28.762591482351667	21.660050230900104	22.216370952496693	27.360987334251536
26	28.620810715925355	22.583650652191526	21.68840638418537	27.107132247697752
27	27.19220070755354	21.697858435280455	24.526722298738825	26.58321855842718
28	26.78576251046477	23.206135731453724	21.176645332037054	28.83145642604445
29	27.537875719031028	23.26284803802425	21.44940452078101	27.74987172216371
30	27.061222285235896	21.70596019336196	24.02846417672635	27.2043533446758
31	28.739636501120742	21.854492424856193	22.33924761673283	27.066623457290234
32	26.02824812984418	21.479110967079855	22.360852304950175	30.13178859812579
33	26.11196629668638	21.560128547894895	24.321477760674064	28.00642739474466
34	26.278052337357206	21.55607766885414	23.643630667854925	28.52223932593373
35	26.00664344162683	23.276350968160088	24.229657835750356	26.48734775446272
36	26.71014610170407	21.387291042156146	24.763023576116016	27.139539280023765
37	27.03961759701855	21.187447676145723	24.120284101650057	27.652650625185665
38	25.862162089173353	20.408328607307784	25.955332307110645	27.774176996408222
39	25.44762213400308	19.33214507548138	27.078776094412486	28.141456696103056
40	24.838639984876718	20.53255556455751	28.21572281185017	26.413081638715603
41	22.984687677225956	19.49958140916579	29.120419130951415	28.395311782656833
42	21.198250020254395	20.65543222879365	30.562532069459074	27.583785681492884
43	20.031596856517865	21.09427745820843	29.859029409381836	29.015096275891867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	3.5
7	3.0
8	5.0
9	7.0
10	10.5
11	14.0
12	14.0
13	27.5
14	41.0
15	68.0
16	95.0
17	94.0
18	93.0
19	93.0
20	99.0
21	105.0
22	100.0
23	95.0
24	110.5
25	126.0
26	126.0
27	139.5
28	153.0
29	191.0
30	229.0
31	280.0
32	331.0
33	331.0
34	417.5
35	504.0
36	597.0
37	690.0
38	918.5
39	1147.0
40	1147.0
41	1488.5
42	1830.0
43	2255.0
44	2680.0
45	4019.0
46	5358.0
47	5358.0
48	6216.0
49	7074.0
50	7841.5
51	8609.0
52	8807.0
53	9005.0
54	9005.0
55	8508.5
56	8012.0
57	7717.5
58	7423.0
59	6883.0
60	6343.0
61	6343.0
62	5643.5
63	4944.0
64	3985.0
65	3026.0
66	2588.5
67	2151.0
68	2151.0
69	1813.0
70	1475.0
71	1223.5
72	972.0
73	763.5
74	555.0
75	555.0
76	440.5
77	326.0
78	274.0
79	222.0
80	201.0
81	180.0
82	180.0
83	145.5
84	111.0
85	86.5
86	62.0
87	48.0
88	34.0
89	34.0
90	27.0
91	20.0
92	13.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	74058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.29236544330119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93393940847706	48.37424721164493
2	6.303821151862602	7.09714007939723
3	2.319556717599367	3.9172000324070324
4	1.2161481445944973	2.738394231548246
5	0.8371512869102161	2.356261308703989
6	0.5181222864544605	1.749979745604796
7	0.45575571493679384	1.7958897080666507
8	0.36220585766029406	1.6311539604094087
9	0.2134855717335508	1.081584703880742
>10	1.6215308594593298	18.16684220475843
>50	0.1535177145050253	5.871074023063005
>100	0.06476528580680756	5.220232790515541
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	320	0.4320937643468633	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	240	0.32407032326014745	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	181	0.24440303545869455	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	175	0.23630127737719084	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	169	0.22819951929568716	TruSeq Adapter, Index 12 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	168	0.22684922628210322	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	159	0.21469658915984768	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	154	0.20794512409192792	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	152	0.20524453806476006	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	151	0.2038942450511761	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	146	0.19714277998325636	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	138	0.1863404358745848	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	128	0.17283750573874532	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	127	0.17148721272516138	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	127	0.17148721272516138	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	127	0.17148721272516138	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	124	0.16743633368440952	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	118	0.15933457560290581	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	116	0.15663398957573793	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	116	0.15663398957573793	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	115	0.155283696562154	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	104	0.14043047341273057	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	103	0.13908018039914663	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	103	0.13908018039914663	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	103	0.13908018039914663	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	101	0.13637959437197872	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	101	0.13637959437197872	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	99	0.13367900834481083	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	98	0.13232871533122686	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	97	0.13097842231764292	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	97	0.13097842231764292	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	96	0.12962812930405898	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	96	0.12962812930405898	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	96	0.12962812930405898	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	94	0.12692754327689107	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	90	0.12152637122255529	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	85	0.11477490615463555	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	85	0.11477490615463555	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	83	0.11207432012746767	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	81	0.10937373410029975	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	81	0.10937373410029975	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	81	0.10937373410029975	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	79	0.10667314807313187	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	78	0.10532285505954792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0013502930135839478	0.0
17	0.0	0.0	0.0	0.004050879040751843	0.0
18	0.0	0.0	0.0	0.004050879040751843	0.0
19	0.0	0.0	0.0	0.004050879040751843	0.0
20	0.0	0.0	0.0	0.004050879040751843	0.0
21	0.0	0.0	0.0	0.009452051095087633	0.0
22	0.0	0.0	0.0	0.01215263712225553	0.0
23	0.0	0.0	0.0	0.013502930135839477	0.0
24	0.0	0.0	0.0	0.014853223149423425	0.0
25	0.0	0.0	0.0	0.01755380917659132	0.0
26	0.0	0.0	0.0	0.027005860271678953	0.0
27	0.0	0.0	0.0	0.06211347862486159	0.0
28	0.0	0.0	0.0	0.18904102190175268	0.0
29	0.0	0.0	0.0	0.38618380188500906	0.0
30	0.0	0.0	0.0	0.644089767479543	0.0
31	0.0	0.0	0.0	1.2530719166059034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	30	8.207362E-6	37.0	5
GTATACG	30	8.207362E-6	37.0	4
GACGTAT	35	2.3584864E-5	31.714285	1
ACGGTGT	35	2.3584864E-5	31.714285	8
ATGGGCA	25	0.0054636085	29.6	5
CTCTATG	25	0.0054636085	29.6	1
GGTATCA	150	0.0	29.6	1
CCCGGTG	40	5.866553E-5	27.75	23
TGCCGGA	40	5.866553E-5	27.75	28
ACGTATA	40	5.866553E-5	27.75	2
GACGCCT	40	5.866553E-5	27.75	15
ATACGGT	40	5.866553E-5	27.75	6
CGTATAC	40	5.866553E-5	27.75	3
GCCCGGT	40	5.866553E-5	27.75	22
TTATACA	35	8.790948E-4	26.42857	37
CGCCCTA	50	8.942068E-6	25.900002	16
CCGCCCT	50	8.942068E-6	25.900002	15
GTCCCGC	50	8.942068E-6	25.900002	12
CGTGTCC	50	8.942068E-6	25.900002	9
TGCCCGG	45	1.3069535E-4	24.666668	21
>>END_MODULE