FastQCFastQC Report
Fri 10 Feb 2017
ERR1632443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.5260081823495032No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT60.3506721215663355No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT60.3506721215663355RNA PCR Primer, Index 17 (95% over 21bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.29222676797194624No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT50.29222676797194624No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC50.29222676797194624No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC50.29222676797194624No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC50.29222676797194624RNA PCR Primer, Index 17 (95% over 22bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA50.29222676797194624No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.23378141437755698No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG40.23378141437755698No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC40.23378141437755698No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA40.23378141437755698No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA40.23378141437755698No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.23378141437755698No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA40.23378141437755698No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40.23378141437755698No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.17533606078316774No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGT30.17533606078316774No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG30.17533606078316774No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACG30.17533606078316774No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA30.17533606078316774No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA30.17533606078316774No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA30.17533606078316774No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC30.17533606078316774No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT30.17533606078316774No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT30.17533606078316774No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC30.17533606078316774No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT30.17533606078316774No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC30.17533606078316774No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA30.17533606078316774No Hit
GCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAG30.17533606078316774No Hit
CCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCT20.11689070718877849No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT20.11689070718877849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11689070718877849No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA20.11689070718877849No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC20.11689070718877849No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG20.11689070718877849No Hit
GTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGG20.11689070718877849No Hit
GTGAGGGCCTCACCCCAACGCAGCCTTAGCTTCCTCGGAAGGA20.11689070718877849No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA20.11689070718877849No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCC20.11689070718877849No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCT20.11689070718877849No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC20.11689070718877849No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA20.11689070718877849No Hit
GCGCACTACAGCCCAGAACTCCTGGGCTCAAGCGATCCTCCCA20.11689070718877849No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC20.11689070718877849No Hit
CTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGC20.11689070718877849No Hit
TATCAACGCAGAGTACGGGATTGCACAATGGGCGCAAGCCTGA20.11689070718877849No Hit
GCCCAGAACTCCTGGGCTCAAGCGATCCTCCCACCTCAGCCTC20.11689070718877849No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG20.11689070718877849No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG20.11689070718877849No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGC20.11689070718877849No Hit
CCATGAGGGTGGGCACGCCGCCGATGCGATCGCCAACGAAGCC20.11689070718877849No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG20.11689070718877849No Hit
TTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGCTGTC20.11689070718877849No Hit
GTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACAC20.11689070718877849No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGCTG20.11689070718877849No Hit
CCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTAC20.11689070718877849No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA20.11689070718877849No Hit
CGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGT20.11689070718877849No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT20.11689070718877849No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT20.11689070718877849No Hit
CACCGAGGCAATCGCACTTCATGGTCACTAAAGACTGTCTCTT20.11689070718877849No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT20.11689070718877849No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT20.11689070718877849No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC20.11689070718877849No Hit
CCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAA20.11689070718877849No Hit
CCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATC20.11689070718877849No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC20.11689070718877849No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG20.11689070718877849No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC20.11689070718877849No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA20.11689070718877849No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA20.11689070718877849No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG20.11689070718877849No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAA20.11689070718877849No Hit
TCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTT20.11689070718877849No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG20.11689070718877849No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11689070718877849No Hit
GCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTA20.11689070718877849No Hit
GGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTGTCTCTT20.11689070718877849No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGG20.11689070718877849No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT20.11689070718877849No Hit
GAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAA20.11689070718877849No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAAT20.11689070718877849No Hit
ATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCA20.11689070718877849No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGC20.11689070718877849No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTG20.11689070718877849No Hit
ATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG20.11689070718877849No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC20.11689070718877849No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT20.11689070718877849No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT20.11689070718877849No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA20.11689070718877849No Hit
GATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA20.11689070718877849No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC20.11689070718877849No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGG20.11689070718877849No Hit
GGTATCAACGCAGAGTACGGGTGGGCGCAAGCCTGATGCAGCC20.11689070718877849No Hit
CACTAAAGATGACGGAGCCCGTACTCTGCGTTGATACCCTGTC20.11689070718877849No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT20.11689070718877849No Hit
TCGCAGATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCA20.11689070718877849No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC20.11689070718877849No Hit
GTCCGAGGCAGTCCTGCCATCAATGTGGCCGTGCATGTGTTCA20.11689070718877849No Hit
TTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTAT20.11689070718877849No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT20.11689070718877849No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG20.11689070718877849No Hit
CTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGC20.11689070718877849No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC20.11689070718877849No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20.11689070718877849No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA20.11689070718877849No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)