FastQCFastQC Report
Fri 10 Feb 2017
ERR1632442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT140.47814207650273227TruSeq Adapter, Index 11 (95% over 23bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT130.44398907103825136No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA110.3756830601092896No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC100.34153005464480873No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA90.3073770491803279No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA90.3073770491803279No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA80.273224043715847No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG70.23907103825136614No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG70.23907103825136614No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT70.23907103825136614No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA70.23907103825136614No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC70.23907103825136614No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA60.20491803278688525No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT60.20491803278688525No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG60.20491803278688525No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.20491803278688525No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG60.20491803278688525No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT60.20491803278688525No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA60.20491803278688525No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC50.17076502732240437No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA50.17076502732240437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.17076502732240437No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.17076502732240437No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC50.17076502732240437No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG50.17076502732240437No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC40.1366120218579235No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA40.1366120218579235No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT40.1366120218579235No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT40.1366120218579235No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT40.1366120218579235No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40.1366120218579235No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG40.1366120218579235No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.1366120218579235No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC40.1366120218579235No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC40.1366120218579235No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG40.1366120218579235No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA40.1366120218579235No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC40.1366120218579235No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG40.1366120218579235No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC40.1366120218579235No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT40.1366120218579235No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA40.1366120218579235No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC40.1366120218579235No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC40.1366120218579235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC40.1366120218579235No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC40.1366120218579235No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA40.1366120218579235No Hit
GATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA40.1366120218579235No Hit
CAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGC40.1366120218579235No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA40.1366120218579235No Hit
ACACTGAACATTGAGCCTTGATGTGTAGGATAGGTGGGAGGCT40.1366120218579235No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT40.1366120218579235No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA40.1366120218579235No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATT40.1366120218579235No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT40.1366120218579235No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA40.1366120218579235No Hit
CTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCCGAACCT30.10245901639344263No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC30.10245901639344263No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG30.10245901639344263No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA30.10245901639344263No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA30.10245901639344263No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA30.10245901639344263No Hit
GTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAA30.10245901639344263No Hit
CCGGTGACACAGCCCAGGGCCAGGACGATGGACAGCGCAGCCA30.10245901639344263No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGCT30.10245901639344263No Hit
CCTTAGGGGTCGACTCACCCTGCCCCGATTAACGTTGGACAGG30.10245901639344263No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG30.10245901639344263No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA30.10245901639344263No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG30.10245901639344263No Hit
GAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAAGATC30.10245901639344263No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG30.10245901639344263No Hit
GCGTCACACCGTATACGTCCACTTTCGTGTTTGCACAGTGCTG30.10245901639344263No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC30.10245901639344263RNA PCR Primer, Index 11 (95% over 24bp)
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGAT30.10245901639344263No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC30.10245901639344263No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT30.10245901639344263No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG30.10245901639344263No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTG30.10245901639344263No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG30.10245901639344263No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA30.10245901639344263No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT30.10245901639344263No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC30.10245901639344263No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT30.10245901639344263TruSeq Adapter, Index 11 (95% over 22bp)
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA30.10245901639344263No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATA30.10245901639344263No Hit
GGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTGCATGG30.10245901639344263No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG30.10245901639344263No Hit
GGTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACAC30.10245901639344263No Hit
GCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTA30.10245901639344263No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA30.10245901639344263No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACCT30.10245901639344263No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC30.10245901639344263TruSeq Adapter, Index 11 (95% over 21bp)
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG30.10245901639344263No Hit
CCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGA30.10245901639344263No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC30.10245901639344263No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGA30.10245901639344263No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA30.10245901639344263No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCTGTC30.10245901639344263No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG30.10245901639344263No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC30.10245901639344263No Hit
CGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGA30.10245901639344263No Hit
CGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGC30.10245901639344263No Hit
GCCGAGATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGC30.10245901639344263No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC30.10245901639344263No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers