FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | ERR1632441.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 5548 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 43 |
| %GC | 54 |
Per base sequence quality
Per sequence quality scores
![[WARN]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT | 53 | 0.955299206921413 | TruSeq Adapter, Index 11 (95% over 21bp) |
| ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA | 29 | 0.5227108868060562 | No Hit |
| CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA | 24 | 0.43258832011535686 | No Hit |
| TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA | 23 | 0.414563806777217 | No Hit |
| GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT | 19 | 0.3424657534246575 | No Hit |
| GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA | 19 | 0.3424657534246575 | No Hit |
| GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA | 18 | 0.32444124008651765 | No Hit |
| GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA | 18 | 0.32444124008651765 | No Hit |
| GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC | 18 | 0.32444124008651765 | No Hit |
| AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA | 17 | 0.3064167267483778 | No Hit |
| CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC | 17 | 0.3064167267483778 | No Hit |
| GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC | 16 | 0.2883922134102379 | No Hit |
| GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT | 15 | 0.27036770007209804 | No Hit |
| CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC | 14 | 0.25234318673395817 | No Hit |
| GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC | 14 | 0.25234318673395817 | No Hit |
| CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA | 14 | 0.25234318673395817 | No Hit |
| CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA | 14 | 0.25234318673395817 | No Hit |
| GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG | 13 | 0.2343186733958183 | No Hit |
| CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC | 12 | 0.21629416005767843 | RNA PCR Primer, Index 11 (95% over 22bp) |
| ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG | 12 | 0.21629416005767843 | No Hit |
| GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC | 12 | 0.21629416005767843 | No Hit |
| CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA | 12 | 0.21629416005767843 | No Hit |
| CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT | 12 | 0.21629416005767843 | No Hit |
| GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC | 11 | 0.19826964671953856 | No Hit |
| GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT | 11 | 0.19826964671953856 | No Hit |
| TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT | 11 | 0.19826964671953856 | No Hit |
| ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC | 11 | 0.19826964671953856 | No Hit |
| CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT | 10 | 0.1802451333813987 | No Hit |
| GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG | 10 | 0.1802451333813987 | No Hit |
| CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA | 10 | 0.1802451333813987 | No Hit |
| CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA | 10 | 0.1802451333813987 | No Hit |
| CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC | 10 | 0.1802451333813987 | No Hit |
| CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT | 10 | 0.1802451333813987 | No Hit |
| GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG | 9 | 0.16222062004325882 | No Hit |
| GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG | 9 | 0.16222062004325882 | No Hit |
| GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC | 9 | 0.16222062004325882 | No Hit |
| CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG | 9 | 0.16222062004325882 | No Hit |
| CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT | 9 | 0.16222062004325882 | No Hit |
| CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC | 9 | 0.16222062004325882 | No Hit |
| GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA | 9 | 0.16222062004325882 | No Hit |
| ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA | 9 | 0.16222062004325882 | No Hit |
| CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT | 9 | 0.16222062004325882 | No Hit |
| ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA | 9 | 0.16222062004325882 | No Hit |
| GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC | 8 | 0.14419610670511895 | No Hit |
| GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA | 8 | 0.14419610670511895 | No Hit |
| GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC | 8 | 0.14419610670511895 | No Hit |
| GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC | 8 | 0.14419610670511895 | No Hit |
| GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC | 8 | 0.14419610670511895 | No Hit |
| ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG | 8 | 0.14419610670511895 | No Hit |
| ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA | 8 | 0.14419610670511895 | No Hit |
| GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA | 8 | 0.14419610670511895 | No Hit |
| GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA | 8 | 0.14419610670511895 | No Hit |
| GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG | 8 | 0.14419610670511895 | No Hit |
| ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT | 8 | 0.14419610670511895 | No Hit |
| TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA | 8 | 0.14419610670511895 | No Hit |
| CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCT | 7 | 0.12617159336697908 | No Hit |
| GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT | 7 | 0.12617159336697908 | No Hit |
| CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC | 7 | 0.12617159336697908 | No Hit |
| GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC | 7 | 0.12617159336697908 | No Hit |
| TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC | 7 | 0.12617159336697908 | No Hit |
| AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA | 7 | 0.12617159336697908 | No Hit |
| GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG | 7 | 0.12617159336697908 | No Hit |
| GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC | 7 | 0.12617159336697908 | No Hit |
| CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG | 7 | 0.12617159336697908 | No Hit |
| TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC | 7 | 0.12617159336697908 | No Hit |
| TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7 | 0.12617159336697908 | No Hit |
| GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT | 7 | 0.12617159336697908 | No Hit |
| CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT | 7 | 0.12617159336697908 | No Hit |
| CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT | 7 | 0.12617159336697908 | No Hit |
| GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG | 7 | 0.12617159336697908 | No Hit |
| GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 7 | 0.12617159336697908 | No Hit |
| CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA | 7 | 0.12617159336697908 | No Hit |
| GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG | 7 | 0.12617159336697908 | No Hit |
| CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC | 7 | 0.12617159336697908 | No Hit |
| CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG | 7 | 0.12617159336697908 | No Hit |
| GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA | 7 | 0.12617159336697908 | No Hit |
| CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT | 6 | 0.10814708002883922 | No Hit |
| AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG | 6 | 0.10814708002883922 | No Hit |
| GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC | 6 | 0.10814708002883922 | No Hit |
| AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC | 6 | 0.10814708002883922 | No Hit |
| GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT | 6 | 0.10814708002883922 | No Hit |
| GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG | 6 | 0.10814708002883922 | No Hit |
| CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT | 6 | 0.10814708002883922 | No Hit |
| CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA | 6 | 0.10814708002883922 | No Hit |
| GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA | 6 | 0.10814708002883922 | No Hit |
| CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA | 6 | 0.10814708002883922 | No Hit |
| AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG | 6 | 0.10814708002883922 | No Hit |
| GTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACG | 6 | 0.10814708002883922 | No Hit |
| GGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTC | 6 | 0.10814708002883922 | No Hit |
| GGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCT | 6 | 0.10814708002883922 | No Hit |
| GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC | 6 | 0.10814708002883922 | No Hit |
| GCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGATGA | 6 | 0.10814708002883922 | No Hit |
| TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT | 6 | 0.10814708002883922 | No Hit |
| GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC | 6 | 0.10814708002883922 | No Hit |
| GGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTA | 6 | 0.10814708002883922 | No Hit |
| GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC | 6 | 0.10814708002883922 | No Hit |
| TGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGT | 6 | 0.10814708002883922 | No Hit |
| CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC | 6 | 0.10814708002883922 | No Hit |
| GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG | 6 | 0.10814708002883922 | No Hit |
| CCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTT | 6 | 0.10814708002883922 | No Hit |
| GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC | 6 | 0.10814708002883922 | No Hit |
| CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT | 6 | 0.10814708002883922 | No Hit |
| AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG | 6 | 0.10814708002883922 | No Hit |
Adapter Content
Kmer Content
No overrepresented Kmers