FastQCFastQC Report
Fri 10 Feb 2017
ERR1632440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14331
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT560.39076128672109417No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA300.20933640360058614No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA280.19538064336054708No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC270.18840276324052752No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA260.181424883120508No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA250.17444700300048846No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.13955760240039075No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT200.13955760240039075No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC180.1256018421603517No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA180.1256018421603517No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG160.1116460819203126No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA150.10466820180029307No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC150.10466820180029307Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT150.10466820180029307No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC150.10466820180029307No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC150.10466820180029307No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA150.10466820180029307No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)