FastQCFastQC Report
Fri 10 Feb 2017
ERR1632439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159926
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT3310.20697072395983143No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT3150.19696609681977914No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2850.17820742093218114No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1940.12130610407313383No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1800.11255205532558808No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC1720.10754974175556195No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1650.10317271738178907No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1630.10192213898928254No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1620.10129684979302929No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1610.10067156059677601No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1600.10004627140052275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC200.001837201337.03
GTGGAGT303.5857572E-430.83333424
ATCAGAA1450.028.06896817
GGTATCA1800.027.7500021
CATCAGA1550.027.45161416
TCAGAAG1550.026.25806418
TTCAAGG659.2864866E-825.6153852
GACAGGC1600.025.4375027
GGACAGG1600.025.4375026
CCAGGAC1700.025.0294113
AGAAGAG1650.024.66666820
ACATCGA451.316265E-424.66666820
AGGCCAT1700.023.94117725
GTGCTTC551.8894123E-523.54545411
AATGAGC551.8894123E-523.54545434
GTAACGT551.8894123E-523.54545426
ATACACA400.001923963923.12500237
TCACGTA400.001923963923.12500225
ATACGGA903.767127E-922.61111329
ATCAAGC1650.022.42424430
Produced by FastQC (version 0.11.5)