##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632439.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	159926
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.034622262796546	34.0	33.0	34.0	31.0	34.0
2	33.17465577829746	34.0	33.0	34.0	31.0	34.0
3	33.243937821242326	34.0	34.0	34.0	31.0	34.0
4	36.53287770593899	37.0	37.0	37.0	35.0	37.0
5	36.503113940197345	37.0	37.0	37.0	35.0	37.0
6	36.54171929517402	37.0	37.0	37.0	35.0	37.0
7	36.542851068619235	37.0	37.0	37.0	35.0	37.0
8	36.51489438865475	37.0	37.0	37.0	35.0	37.0
9	38.30307767342396	39.0	39.0	39.0	37.0	39.0
10	38.33389817790728	39.0	39.0	39.0	37.0	39.0
11	38.41208433900679	39.0	39.0	39.0	37.0	39.0
12	38.36599427235096	39.0	39.0	39.0	37.0	39.0
13	38.39691482310569	39.0	39.0	39.0	37.0	39.0
14	39.95201530707953	41.0	40.0	41.0	38.0	41.0
15	39.94452434250841	41.0	40.0	41.0	38.0	41.0
16	39.91619874191814	41.0	40.0	41.0	38.0	41.0
17	39.91022097720196	41.0	40.0	41.0	38.0	41.0
18	39.86556282280555	41.0	40.0	41.0	38.0	41.0
19	39.87023998599352	41.0	40.0	41.0	38.0	41.0
20	39.84715430886785	41.0	40.0	41.0	38.0	41.0
21	39.83100308892863	41.0	40.0	41.0	38.0	41.0
22	39.80639795905606	41.0	40.0	41.0	38.0	41.0
23	39.76108325100359	41.0	40.0	41.0	38.0	41.0
24	39.738504058126885	41.0	40.0	41.0	38.0	41.0
25	39.69452746895439	41.0	40.0	41.0	37.0	41.0
26	39.60673061290847	41.0	40.0	41.0	37.0	41.0
27	39.533840651301226	41.0	40.0	41.0	37.0	41.0
28	39.47844002851319	41.0	40.0	41.0	37.0	41.0
29	39.442604704675915	41.0	39.0	41.0	37.0	41.0
30	39.3893863411828	41.0	39.0	41.0	36.0	41.0
31	39.33716844040369	41.0	39.0	41.0	36.0	41.0
32	39.289183747483214	41.0	39.0	41.0	36.0	41.0
33	39.224697672673614	41.0	39.0	41.0	35.0	41.0
34	39.168021459925214	41.0	39.0	41.0	35.0	41.0
35	39.08420769605943	41.0	39.0	41.0	35.0	41.0
36	39.050623413328665	41.0	39.0	41.0	35.0	41.0
37	39.00158823455848	41.0	39.0	41.0	35.0	41.0
38	38.92263296774758	40.0	39.0	41.0	35.0	41.0
39	38.845090854520215	40.0	39.0	41.0	35.0	41.0
40	38.74330002626215	40.0	38.0	41.0	35.0	41.0
41	38.69557170191214	40.0	38.0	41.0	35.0	41.0
42	38.64613008516439	40.0	38.0	41.0	35.0	41.0
43	37.90503107687305	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	4.0
20	8.0
21	20.0
22	27.0
23	54.0
24	71.0
25	122.0
26	209.0
27	285.0
28	373.0
29	520.0
30	742.0
31	945.0
32	1230.0
33	1696.0
34	2515.0
35	4277.0
36	6665.0
37	12096.0
38	31207.0
39	96857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91781198804447	15.412753398446782	11.744806973225117	28.924627640283628
2	21.72442254542726	17.763840776359068	31.628378124882754	28.88335855333092
3	21.94827607768593	18.68676763002889	27.444568112752147	31.92038817953303
4	16.83466103072671	14.457936795768042	31.3213611295224	37.38604104398284
5	18.391631129397346	31.986043545139626	31.79157860510486	17.830746720358164
6	37.837499843677705	31.210059652589322	14.234083263509373	16.718357240223604
7	32.31369508397634	25.962632717631905	18.799319685354476	22.92435251303728
8	28.952140364918776	30.225229168490426	18.52419243900304	22.298438027587757
9	28.373122569188247	12.776534147043005	16.437602391105887	42.41274089266286
10	20.465090104173182	22.446631567099786	28.724535097482583	28.36374323124445
11	40.35679001538212	18.72491027100034	17.88139514525468	23.036904568362868
12	24.642021935145006	22.265297700186338	25.388617235471404	27.704063129197255
13	34.64477320760852	16.859672598576843	20.07991195928117	28.415642234533472
14	25.0528369370834	18.708027462701498	21.570601403148956	34.668534197066144
15	30.31402023435839	22.816177482085465	19.26140840138564	27.608393882170507
16	27.655290571889502	22.716756499881193	20.272501031727174	29.35545189650213
17	28.071733176594176	22.75427385165639	20.93593286895189	28.238060102797547
18	27.026249640458712	20.526368445406	22.826182109225517	29.62119980490977
19	29.24977802233533	21.070995335342595	21.675649988119506	28.00357665420257
20	29.175368607981188	20.38817953303403	22.346585295699263	28.089866563285522
21	29.462376349061444	20.994710053399697	20.81962907844878	28.723284519090083
22	29.937596138213923	21.98266698347986	20.071783199729875	28.007953678576342
23	29.506771881995427	21.80508485174393	20.68144016607681	28.006703100183834
24	29.721246076310294	20.79586808899116	20.612658354488953	28.8702274802096
25	29.06531770944062	20.939059314933157	21.769443367557496	28.22617960806873
26	29.440491227192577	21.399897452571814	21.636882057951805	27.522729262283807
27	29.599314683040905	20.59577554619011	22.12460763102935	27.68030213973963
28	27.402048447406923	21.516826532271175	21.98641871865738	29.09470630166452
29	29.047809611945524	21.620624538849217	21.54809099208384	27.78347485712142
30	27.899153358428276	20.540750096919822	23.095056463614423	28.46504008103748
31	28.241186548778813	21.035979140352413	21.940772607330892	28.782061703537888
32	26.51914010229731	21.22669234520966	22.42474644523092	29.82942110726211
33	27.950427072521038	20.980953691082124	23.093805885221915	27.97481335117492
34	27.599639833422955	21.095381613996473	23.014394157297748	28.29058439528282
35	27.729074697047384	21.892000050023135	23.33954453934945	27.03938071358003
36	27.457073896677215	20.505108612733387	23.182596951089877	28.85522053949952
37	26.24338756674962	20.31064367269862	24.531971036604432	28.913997723947325
38	26.791140902667482	19.193251878994037	25.610594900141315	28.405012318197166
39	25.20853394695046	18.611732926478496	26.99185873466478	29.187874391906256
40	24.92090091667396	19.246401460675564	28.441279091579858	27.39141853107062
41	23.46585295699261	18.442904843490115	29.049060190338032	29.042182009179246
42	21.759438740417444	19.599064567362408	29.821292347710816	28.820204344509335
43	21.363630679189125	19.279541788076983	30.070157447819618	29.286670084914274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	2.5
4	5.0
5	5.0
6	5.5
7	6.0
8	6.0
9	6.0
10	11.0
11	16.0
12	16.0
13	37.5
14	59.0
15	91.0
16	123.0
17	134.0
18	145.0
19	145.0
20	152.0
21	159.0
22	135.5
23	112.0
24	138.0
25	164.0
26	164.0
27	192.0
28	220.0
29	302.5
30	385.0
31	569.5
32	754.0
33	754.0
34	1068.5
35	1383.0
36	1640.5
37	1898.0
38	2582.0
39	3266.0
40	3266.0
41	3797.5
42	4329.0
43	5186.0
44	6043.0
45	7435.5
46	8828.0
47	8828.0
48	10178.0
49	11528.0
50	13138.0
51	14748.0
52	16151.0
53	17554.0
54	17554.0
55	17098.0
56	16642.0
57	16042.5
58	15443.0
59	14501.5
60	13560.0
61	13560.0
62	13016.0
63	12472.0
64	10290.0
65	8108.0
66	7024.0
67	5940.0
68	5940.0
69	5108.5
70	4277.0
71	3869.0
72	3461.0
73	3482.5
74	3504.0
75	3504.0
76	3308.5
77	3113.0
78	2059.0
79	1005.0
80	669.0
81	333.0
82	333.0
83	248.0
84	163.0
85	123.5
86	84.0
87	62.5
88	41.0
89	41.0
90	36.5
91	32.0
92	19.5
93	7.0
94	6.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	159926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.89179995748034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.28432362189193	45.96125708140015
2	6.477775070486268	6.981979165363981
3	2.3379395964588627	3.7798731913509998
4	1.2275633216146287	2.6462238785438266
5	0.8655597711951919	2.3323287020246863
6	0.6207432675461496	2.0071783199729873
7	0.4269785466485665	1.6107449695484162
8	0.3782472994767192	1.6307542238285206
9	0.2923874830310836	1.4181558971024097
>10	1.8274217689442724	19.840426197116166
>50	0.21232900553447734	7.825494291109639
>100	0.048731247171847264	3.9655840826382205
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	331	0.20697072395983143	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	315	0.19696609681977914	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	285	0.17820742093218114	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	194	0.12130610407313383	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	180	0.11255205532558808	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	172	0.10754974175556195	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	165	0.10317271738178907	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	163	0.10192213898928254	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	162	0.10129684979302929	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	161	0.10067156059677601	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	160	0.10004627140052275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	6.252891962532671E-4	0.0	0.0	0.0	0.0
12	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
13	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
14	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
15	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
16	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
17	6.252891962532671E-4	0.0	0.0	0.0	6.252891962532671E-4
18	6.252891962532671E-4	0.0	0.0	6.252891962532671E-4	6.252891962532671E-4
19	6.252891962532671E-4	0.0	0.0	0.0012505783925065342	6.252891962532671E-4
20	6.252891962532671E-4	0.0	0.0	0.003126445981266336	6.252891962532671E-4
21	6.252891962532671E-4	0.0	0.0	0.005002313570026137	6.252891962532671E-4
22	6.252891962532671E-4	0.0	0.0	0.005002313570026137	6.252891962532671E-4
23	6.252891962532671E-4	0.0	0.0	0.006878181158785938	6.252891962532671E-4
24	6.252891962532671E-4	0.0	0.0	0.008128759551292473	6.252891962532671E-4
25	6.252891962532671E-4	0.0	0.0	0.009379337943799007	6.252891962532671E-4
26	6.252891962532671E-4	0.0	0.0	0.01750809749509148	6.252891962532671E-4
27	6.252891962532671E-4	0.0	0.0	0.05064842489651464	6.252891962532671E-4
28	6.252891962532671E-4	0.0	0.0	0.2169753510998837	6.252891962532671E-4
29	6.252891962532671E-4	0.0	0.0	0.43457599139602066	6.252891962532671E-4
30	6.252891962532671E-4	0.0	0.0	0.7253354676537899	6.252891962532671E-4
31	6.252891962532671E-4	0.0	0.0	1.4219076322799296	6.252891962532671E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCTAC	20	0.0018372013	37.0	3
GTGGAGT	30	3.5857572E-4	30.833334	24
ATCAGAA	145	0.0	28.068968	17
GGTATCA	180	0.0	27.750002	1
CATCAGA	155	0.0	27.451614	16
TCAGAAG	155	0.0	26.258064	18
TTCAAGG	65	9.2864866E-8	25.615385	2
GACAGGC	160	0.0	25.437502	7
GGACAGG	160	0.0	25.437502	6
CCAGGAC	170	0.0	25.029411	3
AGAAGAG	165	0.0	24.666668	20
ACATCGA	45	1.316265E-4	24.666668	20
AGGCCAT	170	0.0	23.941177	25
GTGCTTC	55	1.8894123E-5	23.545454	11
AATGAGC	55	1.8894123E-5	23.545454	34
GTAACGT	55	1.8894123E-5	23.545454	26
ATACACA	40	0.0019239639	23.125002	37
TCACGTA	40	0.0019239639	23.125002	25
ATACGGA	90	3.767127E-9	22.611113	29
ATCAAGC	165	0.0	22.424244	30
>>END_MODULE