FastQCFastQC Report
Fri 10 Feb 2017
ERR1632438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103508
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2250.21737450245391662No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1330.1284924836727596RNA PCR Primer, Index 29 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1320.1275263747729644No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1160.11206863237624146No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1060.10240754337828961No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1060.10240754337828961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA502.6791522E-422.21
TCATCGA502.6791522E-422.224
CGCGGTA759.132937E-619.73333222
TTATACA951.639637E-719.47368437
GGTAATA801.5936394E-518.525
GCGGTAA801.5936394E-518.523
CCGCGGT801.5936394E-518.521
CGGTAAT801.5936394E-518.524
GCGCTGT500.00699276918.535
ATACGGA752.0444638E-417.26666629
TAATACG904.383416E-516.44444527
GCCGCGG904.383416E-516.44444520
TACGGAG803.3455208E-416.187530
GTATCAA700.002571185815.8571431
AGCCGCG956.960997E-515.57894819
GTAATAC956.960997E-515.57894826
AATACGG956.960997E-515.57894828
CTTATAC1201.8943556E-615.41666636
TCTTATA2107.2759576E-1214.9761937
GCAGCCG1001.0781782E-414.817
Produced by FastQC (version 0.11.5)