##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632438.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103508
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02419136685087	34.0	33.0	34.0	31.0	34.0
2	33.16875990261622	34.0	33.0	34.0	31.0	34.0
3	33.24025196120107	34.0	34.0	34.0	31.0	34.0
4	36.53525331375353	37.0	37.0	37.0	35.0	37.0
5	36.49638675271476	37.0	37.0	37.0	35.0	37.0
6	36.53624840592032	37.0	37.0	37.0	35.0	37.0
7	36.52984310391467	37.0	37.0	37.0	35.0	37.0
8	36.50355528075124	37.0	37.0	37.0	35.0	37.0
9	38.28457703752367	39.0	39.0	39.0	37.0	39.0
10	38.331153147582796	39.0	39.0	39.0	37.0	39.0
11	38.3995439965993	39.0	39.0	39.0	37.0	39.0
12	38.35258144298025	39.0	39.0	39.0	37.0	39.0
13	38.39361208795455	39.0	39.0	39.0	37.0	39.0
14	39.951375739073306	41.0	40.0	41.0	38.0	41.0
15	39.93760868725123	41.0	40.0	41.0	38.0	41.0
16	39.916537852146696	41.0	40.0	41.0	38.0	41.0
17	39.894510569231365	41.0	40.0	41.0	38.0	41.0
18	39.899621285311284	41.0	40.0	41.0	38.0	41.0
19	39.90249062874367	41.0	40.0	41.0	38.0	41.0
20	39.87238667542606	41.0	40.0	41.0	38.0	41.0
21	39.834553850910076	41.0	40.0	41.0	38.0	41.0
22	39.80372531591761	41.0	40.0	41.0	38.0	41.0
23	39.77815241334003	41.0	40.0	41.0	38.0	41.0
24	39.74872473625227	41.0	40.0	41.0	38.0	41.0
25	39.709684275611544	41.0	40.0	41.0	38.0	41.0
26	39.624956525099506	41.0	40.0	41.0	37.0	41.0
27	39.542991846040884	41.0	40.0	41.0	37.0	41.0
28	39.492947405031494	41.0	40.0	41.0	37.0	41.0
29	39.454882714379565	41.0	39.0	41.0	37.0	41.0
30	39.41567801522588	41.0	39.0	41.0	37.0	41.0
31	39.34606020790663	41.0	39.0	41.0	36.0	41.0
32	39.29112725586428	41.0	39.0	41.0	36.0	41.0
33	39.225171001275264	41.0	39.0	41.0	36.0	41.0
34	39.17905862348804	41.0	39.0	41.0	35.0	41.0
35	39.12285040769795	41.0	39.0	41.0	35.0	41.0
36	39.070603238397034	41.0	39.0	41.0	35.0	41.0
37	38.99400046373227	40.0	39.0	41.0	35.0	41.0
38	38.93929937782587	40.0	39.0	41.0	35.0	41.0
39	38.844746299802914	40.0	38.0	41.0	35.0	41.0
40	38.76608571318159	40.0	38.0	41.0	35.0	41.0
41	38.708003246125905	40.0	38.0	41.0	35.0	41.0
42	38.630627584341305	40.0	38.0	41.0	35.0	41.0
43	37.90392046991537	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	7.0
21	12.0
22	25.0
23	51.0
24	59.0
25	90.0
26	128.0
27	179.0
28	261.0
29	340.0
30	475.0
31	561.0
32	847.0
33	982.0
34	1564.0
35	2584.0
36	4143.0
37	7839.0
38	21100.0
39	62257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2981025621208	16.232561734358697	12.123700583529775	28.345635119990725
2	21.820535610774048	18.87003903079955	31.39274258994474	27.916682768481664
3	21.500753564941842	18.925107238087875	28.105074003941727	31.469065193028555
4	16.537852146693975	14.903195888240523	31.91830583143332	36.64064613363218
5	18.347374116010357	32.45063183522047	31.957916296324925	17.244077752444255
6	36.87154616068323	31.971441820922053	14.383429300150713	16.773582718244
7	31.67774471538432	26.83367469181126	19.047803068361866	22.440777524442552
8	27.96305599567183	31.035282297020522	19.086447424353672	21.915214282953972
9	27.705104919426514	13.109131661320864	16.961007844804264	42.22475557444835
10	19.556942458553927	23.312207752057812	29.590949491826716	27.53990029756154
11	39.83170382965568	19.141515631641997	18.376357383004212	22.65042315569811
12	23.61170151099432	22.7528306990764	26.35062024191367	27.284847548015613
13	34.920972291996755	17.15326351586351	20.51532248715075	27.410441704988987
14	24.855083665030723	18.716427715732117	21.89395988715848	34.53452873207868
15	29.555203462534298	23.60880318429493	19.040074197163502	27.795919156007265
16	26.85589519650655	23.303512771959657	21.652432662209684	28.18815936932411
17	27.51188313946748	23.15859643699038	21.523940178536925	27.805580245005217
18	26.88198013680102	21.537465703134057	22.64076206670016	28.939792093364762
19	28.723383699810647	21.859179966765854	22.45333694013989	26.964099393283607
20	29.693357035205008	21.04088572863933	21.982841905939637	27.282915330216024
21	27.826834640800712	21.771264056884494	21.65339877110948	28.748502531205318
22	28.67411214592109	22.19538586389458	20.665069366619004	28.465432623565327
23	28.45673764346717	22.09684275611547	21.590601692622794	27.855817907794567
24	28.400703327279054	21.427329288557406	21.537465703134057	28.634501681029484
25	28.976504231556984	21.634076593113576	21.986706341538817	27.402712833790627
26	28.412296634076593	22.149978745604205	21.65919542450825	27.778529195810954
27	27.783359740309926	21.624415504115625	22.886153727248136	27.706071028326313
28	27.18920276693589	22.262047377980444	21.682382038103334	28.866367816980333
29	27.58530741585192	22.963442439231752	21.902654867256636	27.5485952776597
30	27.628782316342697	22.103605518414035	22.86489933145264	27.402712833790627
31	28.164972755729025	21.906519302855816	22.099741082814855	27.8287668586003
32	26.371874637709166	22.113266607411987	22.474591335935386	29.04026741894346
33	27.03462534296866	21.508482436140202	23.479344591722377	27.977547629168757
34	27.45778104107895	21.26985353789079	23.167291417088535	28.105074003941727
35	26.680063376743828	22.265911813579628	23.64551532248715	27.4085094871894
36	26.885844572400202	21.062140124434826	24.24353673146037	27.808478571704605
37	26.89550566139815	20.711442593809174	24.240638404760983	28.15241334003169
38	25.915871237005835	20.406152181473896	25.837616416122426	27.840360165397843
39	25.395138540016234	19.520230320361712	26.69938555473973	28.38524558488233
40	24.657997449472504	20.078641264443327	27.90315724388453	27.360204042199637
41	23.620396491092478	19.63519727943734	28.412296634076593	28.332109595393597
42	22.654287591297294	20.107624531437185	29.095335626231787	28.142752251033738
43	21.865942729064418	20.434169339567955	28.86250338138115	28.83738454998647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	4.0
7	6.0
8	5.0
9	4.0
10	7.5
11	11.0
12	11.0
13	19.5
14	28.0
15	43.0
16	58.0
17	57.5
18	57.0
19	57.0
20	87.0
21	117.0
22	114.5
23	112.0
24	145.0
25	178.0
26	178.0
27	229.5
28	281.0
29	384.0
30	487.0
31	609.5
32	732.0
33	732.0
34	974.0
35	1216.0
36	1386.0
37	1556.0
38	2022.5
39	2489.0
40	2489.0
41	2866.0
42	3243.0
43	3742.5
44	4242.0
45	5185.0
46	6128.0
47	6128.0
48	7002.5
49	7877.0
50	8885.0
51	9893.0
52	10591.5
53	11290.0
54	11290.0
55	10801.0
56	10312.0
57	9849.0
58	9386.0
59	8902.5
60	8419.0
61	8419.0
62	8004.5
63	7590.0
64	6552.0
65	5514.0
66	4768.0
67	4022.0
68	4022.0
69	3467.5
70	2913.0
71	2511.5
72	2110.0
73	1710.0
74	1310.0
75	1310.0
76	1069.5
77	829.0
78	668.0
79	507.0
80	391.5
81	276.0
82	276.0
83	224.0
84	172.0
85	123.0
86	74.0
87	58.0
88	42.0
89	42.0
90	28.0
91	14.0
92	10.0
93	6.0
94	4.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	103508.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.2697762491788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.2600761981151	64.50805734822428
2	5.606577100461199	8.103721451482011
3	1.7685983557248846	3.834486223287089
4	0.9518080342223113	2.751478146616687
5	0.5627966045050465	2.033659234068864
6	0.3288550230599559	1.425976736097693
7	0.28340351580776685	1.4337056072960546
8	0.20586859167167967	1.1902461645476679
9	0.14170175790388342	0.9216678904046064
>10	0.8314952209076933	10.516095374270588
>50	0.050798743399505374	2.4906287436719867
>100	0.008020854220974535	0.7902770800324612
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	225	0.21737450245391662	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	133	0.1284924836727596	RNA PCR Primer, Index 29 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	132	0.1275263747729644	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	116	0.11206863237624146	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	106	0.10240754337828961	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	106	0.10240754337828961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	9.661088997951849E-4	0.0
18	0.0	0.0	0.0	9.661088997951849E-4	0.0
19	0.0	0.0	0.0	9.661088997951849E-4	0.0
20	0.0	0.0	0.0	9.661088997951849E-4	0.0
21	0.0	0.0	0.0	0.0019322177995903698	0.0
22	9.661088997951849E-4	0.0	0.0	0.002898326699385555	0.0
23	9.661088997951849E-4	0.0	0.0	0.0038644355991807396	0.0
24	9.661088997951849E-4	0.0	0.0	0.0038644355991807396	0.0
25	9.661088997951849E-4	0.0	0.0	0.00579665339877111	0.0
26	9.661088997951849E-4	0.0	0.0	0.00966108899795185	0.0
27	9.661088997951849E-4	0.0	0.0	0.0318815936932411	0.0
28	9.661088997951849E-4	0.0	0.0	0.14974687946825366	0.0
29	9.661088997951849E-4	0.0	0.0	0.308188739034664	0.0
30	9.661088997951849E-4	0.0	0.0	0.5651737063801832	0.0
31	9.661088997951849E-4	0.0	0.0	1.148703481856475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	2.6791522E-4	22.2	1
TCATCGA	50	2.6791522E-4	22.2	24
CGCGGTA	75	9.132937E-6	19.733332	22
TTATACA	95	1.639637E-7	19.473684	37
GGTAATA	80	1.5936394E-5	18.5	25
GCGGTAA	80	1.5936394E-5	18.5	23
CCGCGGT	80	1.5936394E-5	18.5	21
CGGTAAT	80	1.5936394E-5	18.5	24
GCGCTGT	50	0.006992769	18.5	35
ATACGGA	75	2.0444638E-4	17.266666	29
TAATACG	90	4.383416E-5	16.444445	27
GCCGCGG	90	4.383416E-5	16.444445	20
TACGGAG	80	3.3455208E-4	16.1875	30
GTATCAA	70	0.0025711858	15.857143	1
AGCCGCG	95	6.960997E-5	15.578948	19
GTAATAC	95	6.960997E-5	15.578948	26
AATACGG	95	6.960997E-5	15.578948	28
CTTATAC	120	1.8943556E-6	15.416666	36
TCTTATA	210	7.2759576E-12	14.97619	37
GCAGCCG	100	1.0781782E-4	14.8	17
>>END_MODULE