FastQCFastQC Report
Fri 10 Feb 2017
ERR1632437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97337
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2110.21677265582460936No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1500.15410378376157063No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1390.14280283961905546No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1230.12636510268448792No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1080.11095472430833085No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1070.10992736574992037No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1030.1058179315162785No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC990.1017084972826366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA651.8189894E-1234.1538471
ATATGGG352.3660752E-531.71428717
AGAGTCC250.005471958329.59999826
GTCATGG358.810935E-426.4285721
GATACCA358.810935E-426.4285722
GGATACC358.810935E-426.4285721
GCGGTAA1150.024.13043223
CGCGGTA1200.023.12500222
TATCGCG400.001918579623.1257
GCCGTCT400.001918579623.12536
TACACGG400.001918579623.1255
TCTTATA2300.022.52173837
GGTAATA1250.022.225
CGGTAAT1250.022.224
TACCACG502.6775524E-422.1999994
TATGGGG502.6775524E-422.19999918
AATATGG502.6775524E-422.19999916
CTGAATA502.6775524E-422.19999913
TCAGCAC502.6775524E-422.1999993
GGTATCC502.6775524E-422.1999993
Produced by FastQC (version 0.11.5)