FastQCFastQC Report
Fri 10 Feb 2017
ERR1632436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6933
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT841.2115967113803547TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA520.7500360594259339No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA420.6057983556901774No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA360.5192557334487236No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA330.4759844223279966No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC290.4182893408336939RNA PCR Primer, Index 6 (95% over 22bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT240.34617048896581565No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA220.31732294821866436No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC210.3028991778450887No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA210.3028991778450887No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.28847540747151307No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.28847540747151307No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT200.28847540747151307No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT190.2740516370979374No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT180.2596278667243618No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA180.2596278667243618No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC170.2452040963507861No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG170.2452040963507861No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA160.23078032597721046No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA160.23078032597721046No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG160.23078032597721046No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA160.23078032597721046No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC160.23078032597721046No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC160.23078032597721046No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC150.21635655560363476No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA140.20193278523005911No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC140.20193278523005911No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA140.20193278523005911No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC130.18750901485648347No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC130.18750901485648347No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC130.18750901485648347No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA130.18750901485648347No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA130.18750901485648347No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG130.18750901485648347No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA130.18750901485648347No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC120.17308524448290782No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC120.17308524448290782No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT120.17308524448290782No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT120.17308524448290782No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC110.15866147410933218No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG110.15866147410933218No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC110.15866147410933218No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA110.15866147410933218No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT110.15866147410933218No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC110.15866147410933218No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA110.15866147410933218No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC110.15866147410933218No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA110.15866147410933218No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC100.14423770373575653No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC100.14423770373575653No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT100.14423770373575653No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT100.14423770373575653No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC100.14423770373575653No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT100.14423770373575653No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG90.1298139333621809No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT90.1298139333621809No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG90.1298139333621809No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG90.1298139333621809No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC90.1298139333621809No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC90.1298139333621809No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA90.1298139333621809No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG90.1298139333621809No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT90.1298139333621809No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC80.11539016298860523No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT80.11539016298860523No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG80.11539016298860523No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT80.11539016298860523No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA80.11539016298860523No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC80.11539016298860523No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT80.11539016298860523No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG80.11539016298860523No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG80.11539016298860523No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG80.11539016298860523No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT70.10096639261502956No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC70.10096639261502956No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG70.10096639261502956No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA70.10096639261502956No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA70.10096639261502956No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA70.10096639261502956No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC70.10096639261502956No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC70.10096639261502956No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC70.10096639261502956No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT70.10096639261502956No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA70.10096639261502956No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG70.10096639261502956No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC70.10096639261502956No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA70.10096639261502956No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA70.10096639261502956No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC70.10096639261502956No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA70.10096639261502956No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG70.10096639261502956No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT70.10096639261502956No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC70.10096639261502956No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA70.10096639261502956No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers