FastQCFastQC Report
Fri 10 Feb 2017
ERR1632435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74644
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4350.5827661968811961No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2820.3777932586678099No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2770.37109479663469264No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2740.36707571941482237No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2590.3469803333154708No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2460.3295643320293661No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2140.286694175017416No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2010.2692781737313113No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2010.2692781737313113No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1940.2599003268849472No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1850.24784309522533626No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1640.21970955468624404No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1610.2156904774663737No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1580.21167140024650338Illumina PCR Primer Index 10 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1580.21167140024650338No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1560.20899201543325652No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1560.20899201543325652No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1550.2076523230266331No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1530.2049729382133862No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1520.20363324580676279No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1430.1915760141471518No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1430.1915760141471518No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1420.19023632174052837No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1420.19023632174052837No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1400.18755693692728148No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT1390.18621724452065808No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1340.17951878248754086No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1290.17282032045442366No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1290.17282032045442366No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1280.17148062804780023No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1280.17148062804780023No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1220.1634424736080596No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1210.16210278120143615No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1200.16076308879481271No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1180.15808370398156582No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1130.15138524194844863No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1130.15138524194844863No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1130.15138524194844863No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1110.14870585713520176No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1080.14468677991533144No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1060.14200739510208457No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1050.1406677026954611No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT1040.13932801028883768No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1040.13932801028883768No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1020.1366486254755908No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1000.13396924066234392No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA990.1326295482557205No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT980.13128985584909705No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG980.13128985584909705No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA940.1259310862226033No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC930.12459139381597985No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA890.1192326241894861No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT880.11789293178286264No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT870.11655323937623922No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA870.11655323937623922No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT870.11655323937623922No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT850.11387385456299234No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC850.11387385456299234No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG840.1125341621563689No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT840.1125341621563689No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG820.10985477734312202No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC820.10985477734312202No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA810.10851508493649858No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA800.10717539252987514No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC800.10717539252987514No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA790.10583570012325169No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC790.10583570012325169No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT780.10449600771662826No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG780.10449600771662826No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC770.10315631531000481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC200.001830926537.015
GGTATCA1600.030.06251
TATACAC502.6635644E-729.59999837
AGGGTCT250.005463867429.59999836
TACGGCT453.9426104E-628.77777730
ATACGGC453.9426104E-628.77777729
TTCTGCG905.456968E-1226.72222118
TCTGCGG905.456968E-1226.72222119
AACGTCA959.094947E-1225.3157928
GGGTAAC959.094947E-1225.3157924
GCGGTAA2000.024.97499823
CAGCAGC2050.024.36585415
CCAGCAG2050.024.36585414
CGCGGTA2050.024.36585422
TTCTTCT1002.0008883E-1124.0515
TCTTCTG1002.0008883E-1124.0516
GTAACGT1002.0008883E-1124.0526
TGCGGGT1002.0008883E-1124.0521
GGTAACG1002.0008883E-1124.0525
GCGGGTA1002.0008883E-1124.0522
Produced by FastQC (version 0.11.5)