FastQCFastQC Report
Fri 10 Feb 2017
ERR1632434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15018
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT560.37288587028898657TruSeq Adapter, Index 3 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA390.2596883739512585No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA320.21307764016513514No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA320.21307764016513514No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA290.19310161139965376No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT270.1797842588893328No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA250.16646690637901185No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC240.15980823012385137No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT240.15980823012385137No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA240.15980823012385137No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA240.15980823012385137No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG210.13983220135836996No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC200.13317352510320948No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT200.13317352510320948No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG200.13317352510320948No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.126514848848049No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC170.11319749633772805No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA170.11319749633772805No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT160.10653882008256757No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC160.10653882008256757No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA160.10653882008256757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG551.7457236E-523.5454545
TCAACGC551.7457236E-523.5454543
ATCAACG551.7457236E-523.5454542
CAACGCA551.7457236E-523.5454544
ACGCAGA603.4210087E-521.5833326
CAGAGTA603.4210087E-521.5833329
AGTACGG603.4210087E-521.58333212
AGAGTAC603.4210087E-521.58333210
GAGTACG603.4210087E-521.58333211
CGCAGAG603.4210087E-521.5833327
TATCAAC603.4210087E-521.5833321
GCAGAGT656.340501E-519.9230778
CTCTTAT608.67092E-418.537
ACTCCGT500.00672403318.4999986
GTACGGG700.002436175215.85714213
TCTCTTA950.001147861613.63157937
GTCTCTT1350.00124293410.96296236
TGTCTCT1550.00392323279.54838735
CTGTCTC2003.0995545E-49.24999937
Produced by FastQC (version 0.11.5)