##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15018
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.997469703023036	34.0	33.0	34.0	31.0	34.0
2	33.136835797043545	34.0	33.0	34.0	31.0	34.0
3	33.20402184045812	34.0	33.0	34.0	31.0	34.0
4	36.51931016113996	37.0	37.0	37.0	35.0	37.0
5	36.49813557064856	37.0	37.0	37.0	35.0	37.0
6	36.55127180716474	37.0	37.0	37.0	35.0	37.0
7	36.53176188573712	37.0	37.0	37.0	35.0	37.0
8	36.50079904115062	37.0	37.0	37.0	35.0	37.0
9	38.28579038487149	39.0	39.0	39.0	37.0	39.0
10	38.31988280729791	39.0	39.0	39.0	37.0	39.0
11	38.37701424956719	39.0	39.0	39.0	37.0	39.0
12	38.3320015980823	39.0	39.0	39.0	37.0	39.0
13	38.37288587028899	39.0	39.0	39.0	37.0	39.0
14	39.89998668264749	41.0	40.0	41.0	38.0	41.0
15	39.87701424956719	41.0	40.0	41.0	38.0	41.0
16	39.88100945532028	41.0	40.0	41.0	38.0	41.0
17	39.83905979491277	41.0	40.0	41.0	38.0	41.0
18	39.865228392595554	41.0	40.0	41.0	38.0	41.0
19	39.87501664669064	41.0	40.0	41.0	38.0	41.0
20	39.84132374483953	41.0	40.0	41.0	38.0	41.0
21	39.81056066054069	41.0	40.0	41.0	38.0	41.0
22	39.761619390065256	41.0	40.0	41.0	38.0	41.0
23	39.7366493541084	41.0	40.0	41.0	38.0	41.0
24	39.672659475296314	41.0	40.0	41.0	38.0	41.0
25	39.649021174590494	41.0	40.0	41.0	37.0	41.0
26	39.55952856572114	41.0	40.0	41.0	37.0	41.0
27	39.494340125183115	41.0	40.0	41.0	37.0	41.0
28	39.45412172060195	41.0	39.0	41.0	37.0	41.0
29	39.39339459315488	41.0	39.0	41.0	37.0	41.0
30	39.35257690771075	41.0	39.0	41.0	36.0	41.0
31	39.30356905047277	41.0	39.0	41.0	36.0	41.0
32	39.27347183379944	41.0	39.0	41.0	36.0	41.0
33	39.20202423758157	41.0	39.0	41.0	35.0	41.0
34	39.18557730723132	41.0	39.0	41.0	35.0	41.0
35	39.072313224131044	41.0	39.0	41.0	35.0	41.0
36	39.02263949926755	40.0	39.0	41.0	35.0	41.0
37	38.955453455852975	40.0	39.0	41.0	35.0	41.0
38	38.883739512584896	40.0	39.0	41.0	35.0	41.0
39	38.8091623385271	40.0	38.0	41.0	35.0	41.0
40	38.71500865627913	40.0	38.0	41.0	35.0	41.0
41	38.66420295645226	40.0	38.0	41.0	35.0	41.0
42	38.55753096284459	40.0	38.0	41.0	35.0	41.0
43	37.81941669996005	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	1.0
22	2.0
23	4.0
24	8.0
25	17.0
26	29.0
27	23.0
28	37.0
29	56.0
30	73.0
31	82.0
32	109.0
33	180.0
34	237.0
35	382.0
36	581.0
37	1188.0
38	3091.0
39	8916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39579171660674	16.82647489679052	12.977760021307763	27.799973365294978
2	21.534159009188976	19.276867758689573	31.88174190970835	27.307231322413106
3	21.667332534292182	19.436675988813423	29.32481022772673	29.571181249167665
4	15.667865228392596	15.661206552137436	32.92715408176854	35.74377413770142
5	17.43241443601012	33.173525103209485	32.227993074976695	17.1660673858037
6	35.29098415235051	33.493141563457186	14.675722466373687	16.540151817818618
7	30.08389932081502	27.327207351178583	19.616460247702758	22.972433080303638
8	27.720069250233053	30.803036356372353	19.523238780130512	21.953655613264083
9	27.713410573977892	12.558263417232654	17.339192968437874	42.38913304035158
10	19.78958583033693	22.839259555200425	28.432547609535224	28.93860700492742
11	39.865494739645754	19.436675988813423	17.58556398987881	23.112265281662005
12	23.252097483020375	23.45851644693035	26.181915035290988	27.10747103475829
13	35.630576641363696	17.81861765880943	20.23571713943268	26.315088560394194
14	25.00332933812758	19.736316420295648	22.313224131042748	32.947130110534026
15	29.93074976694633	24.210946863763482	19.50992142762019	26.348381941669995
16	26.81448927953123	23.70488746837129	22.013583699560527	27.467039552536953
17	27.566919696364362	22.719403382607535	21.474230922892527	28.239445998135572
18	27.12078838726861	20.901584764948726	22.61286456252497	29.36476228525769
19	29.171660673858035	21.693967239312826	22.353176188573713	26.781195898255426
20	29.94406711945665	20.961512851245175	22.313224131042748	26.781195898255426
21	27.593554401385006	22.499667066187243	21.700625915567983	28.206152616859768
22	29.278199493940605	22.672792648821414	21.141297110134506	26.907710747103476
23	29.18497802636836	21.993607670795047	20.77506991610068	28.046344386735917
24	29.18497802636836	21.527500332933812	21.713943268078307	27.573578372619522
25	29.51125316287122	21.840458116926357	21.933679584498602	26.71460913570382
26	28.745505393527765	22.413104274870154	21.667332534292182	27.174057797309896
27	27.427087495005992	21.986948994539883	24.111066719936076	26.474896790518045
28	27.313889998668266	22.60620588626981	21.953655613264083	28.12624850179784
29	27.78665601278466	23.57837261952324	21.49420695165801	27.14076441603409
30	27.10747103475829	21.21454254894127	24.064455986149955	27.613530430150483
31	28.459182314555864	22.033559728326008	22.313224131042748	27.194033826075376
32	25.609268877347187	22.479691037421762	22.845918231455585	29.065121853775473
33	26.315088560394194	21.25449460647223	24.264216273804767	28.166200559328807
34	26.83446530829671	21.687308563057663	23.68491143960581	27.793314689039818
35	26.92768677586896	22.17339192968438	24.27753362631509	26.621387668131575
36	26.941004128379276	21.37435077906512	24.830203755493407	26.85444133706219
37	26.841123984551874	21.028099613796776	24.59715008656279	27.53362631508856
38	25.595951524836863	20.422160074577175	25.2363830070582	28.745505393527765
39	25.203089625782393	19.456652017578904	27.054201624717006	28.286056731921693
40	24.736982287921162	20.561992275935545	28.139565854308163	26.561459581835127
41	23.232121454254894	19.79624450659209	28.21281129311493	28.75882274603809
42	20.721800506059395	20.581968304701025	30.689838860034623	28.006392329204953
43	21.08802770009322	21.407644160340926	28.61899054467972	28.885337594886135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	2.5
9	2.0
10	2.0
11	2.0
12	2.0
13	4.0
14	6.0
15	14.5
16	23.0
17	18.0
18	13.0
19	13.0
20	18.5
21	24.0
22	17.5
23	11.0
24	21.0
25	31.0
26	31.0
27	30.0
28	29.0
29	43.0
30	57.0
31	62.5
32	68.0
33	68.0
34	97.5
35	127.0
36	159.0
37	191.0
38	242.0
39	293.0
40	293.0
41	348.5
42	404.0
43	501.5
44	599.0
45	819.0
46	1039.0
47	1039.0
48	1181.0
49	1323.0
50	1508.5
51	1694.0
52	1740.5
53	1787.0
54	1787.0
55	1671.0
56	1555.0
57	1488.0
58	1421.0
59	1326.5
60	1232.0
61	1232.0
62	1123.5
63	1015.0
64	838.0
65	661.0
66	566.5
67	472.0
68	472.0
69	411.5
70	351.0
71	303.5
72	256.0
73	191.5
74	127.0
75	127.0
76	107.0
77	87.0
78	71.5
79	56.0
80	40.0
81	24.0
82	24.0
83	20.5
84	17.0
85	12.5
86	8.0
87	7.5
88	7.0
89	7.0
90	4.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15018.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.96763883339992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.18591573665542	68.64429351444933
2	5.735790293277965	8.829404714342788
3	1.8427199584739165	4.254894127047542
4	1.1592698330305389	3.5690504727660137
5	0.5536811142832425	2.1307764016513517
6	0.3114456267843239	1.4382740711146624
7	0.28549182455229694	1.5381542149420695
8	0.1643740808028376	1.012118790784392
9	0.07786140669608098	0.5393527766679984
>10	0.6747988580327018	7.670795045944866
>50	0.008651267410675664	0.37288587028898657
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	56	0.37288587028898657	TruSeq Adapter, Index 3 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	39	0.2596883739512585	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	32	0.21307764016513514	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	32	0.21307764016513514	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	29	0.19310161139965376	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	27	0.1797842588893328	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	25	0.16646690637901185	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	24	0.15980823012385137	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	24	0.15980823012385137	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	24	0.15980823012385137	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	24	0.15980823012385137	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	21	0.13983220135836996	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	20	0.13317352510320948	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	20	0.13317352510320948	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	20	0.13317352510320948	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	19	0.126514848848049	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	17	0.11319749633772805	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	17	0.11319749633772805	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	16	0.10653882008256757	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	16	0.10653882008256757	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	16	0.10653882008256757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.006658676255160474	0.0
26	0.0	0.0	0.0	0.013317352510320948	0.0
27	0.0	0.0	0.0	0.05992808629644426	0.0
28	0.0	0.0	0.0	0.13317352510320948	0.0
29	0.0	0.0	0.0	0.37954454654414704	0.0
30	0.0	0.0	0.0	0.5793048341989613	0.0
31	0.0	0.0	0.0	1.0920229058463178	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	55	1.7457236E-5	23.545454	5
TCAACGC	55	1.7457236E-5	23.545454	3
ATCAACG	55	1.7457236E-5	23.545454	2
CAACGCA	55	1.7457236E-5	23.545454	4
ACGCAGA	60	3.4210087E-5	21.583332	6
CAGAGTA	60	3.4210087E-5	21.583332	9
AGTACGG	60	3.4210087E-5	21.583332	12
AGAGTAC	60	3.4210087E-5	21.583332	10
GAGTACG	60	3.4210087E-5	21.583332	11
CGCAGAG	60	3.4210087E-5	21.583332	7
TATCAAC	60	3.4210087E-5	21.583332	1
GCAGAGT	65	6.340501E-5	19.923077	8
CTCTTAT	60	8.67092E-4	18.5	37
ACTCCGT	50	0.006724033	18.499998	6
GTACGGG	70	0.0024361752	15.857142	13
TCTCTTA	95	0.0011478616	13.631579	37
GTCTCTT	135	0.001242934	10.962962	36
TGTCTCT	155	0.0039232327	9.548387	35
CTGTCTC	200	3.0995545E-4	9.249999	37
>>END_MODULE