FastQCFastQC Report
Fri 10 Feb 2017
ERR1632433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT771.6670274951288158RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA240.5195929854946958No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA230.49794327776575015No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC190.41134444684996757No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC170.3680450313920762RNA PCR Primer, Index 41 (95% over 23bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA170.3680450313920762No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT150.3247456159341849No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA150.3247456159341849No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC140.30309590820523924No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA130.28144620047629354No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT110.23814678501840225No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT110.23814678501840225No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA110.23814678501840225No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA110.23814678501840225No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG110.23814678501840225No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA110.23814678501840225No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA110.23814678501840225No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC100.2164970772894566No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA100.2164970772894566No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC100.2164970772894566No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT90.19484736956051094No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC90.19484736956051094No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC90.19484736956051094No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA90.19484736956051094No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG90.19484736956051094No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC90.19484736956051094No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC90.19484736956051094No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG90.19484736956051094No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC80.17319766183156526No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA80.17319766183156526No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG80.17319766183156526No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG80.17319766183156526No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT80.17319766183156526No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG80.17319766183156526No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT80.17319766183156526No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC80.17319766183156526No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC80.17319766183156526No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA80.17319766183156526No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC80.17319766183156526No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC80.17319766183156526No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT80.17319766183156526No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA80.17319766183156526No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG80.17319766183156526No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT70.15154795410261962No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA70.15154795410261962No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG70.15154795410261962No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT70.15154795410261962No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC70.15154795410261962No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC70.15154795410261962No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC70.15154795410261962No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA70.15154795410261962No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA70.15154795410261962No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC60.12989824637367395No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC60.12989824637367395No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC60.12989824637367395No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA60.12989824637367395No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT60.12989824637367395No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG60.12989824637367395No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA60.12989824637367395No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG60.12989824637367395No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA60.12989824637367395No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGC60.12989824637367395No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC60.12989824637367395No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.12989824637367395No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT60.12989824637367395No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA60.12989824637367395No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT60.12989824637367395No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG60.12989824637367395No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA60.12989824637367395No Hit
ACCCTGTATCGCACGCCTTTCCAGACGCTTCCACTAACACACA60.12989824637367395No Hit
ACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCC60.12989824637367395No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG50.1082485386447283No Hit
ACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC50.1082485386447283No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG50.1082485386447283No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC50.1082485386447283No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT50.1082485386447283No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG50.1082485386447283No Hit
GCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGC50.1082485386447283No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC50.1082485386447283No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC50.1082485386447283No Hit
GCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGATGA50.1082485386447283No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT50.1082485386447283No Hit
TCGCAGATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCA50.1082485386447283No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC50.1082485386447283No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA50.1082485386447283No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA50.1082485386447283No Hit
CAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA50.1082485386447283No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA50.1082485386447283No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG50.1082485386447283No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG50.1082485386447283No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG50.1082485386447283No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA50.1082485386447283No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG50.1082485386447283No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC50.1082485386447283No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG50.1082485386447283No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50.1082485386447283No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC50.1082485386447283No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG50.1082485386447283No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG50.1082485386447283No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT50.1082485386447283No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.1082485386447283No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATT50.1082485386447283No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA50.1082485386447283No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG50.1082485386447283No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers